Electrospun Polysaccharides for Periodontal Tissue Engineering: A Review of Recent Advances and Future Perspectives.

Periodontitis is the leading cause of tooth loss among the adult population. Indeed, conventional treatment modalities have specific challenges such as scar tissue infiltration, the formation of long epithelium junctions, and aesthetic limitations. Therefore, the development of alternative strategies is highly demanded. In this regard, periodontal tissue engineering has shed new light on the next generation of treatment methods. The essence of this technology is the use of appropriate biomaterials, bioactive agents, and fabrication technologies to mimic the natural extracellular matrix (ECM) of periodontal tissue. Among different candidates, the use of electrospun polysaccharides has gained significant attention. These structures possess a solid resemblance to native periodontal tissue's ECM in physicochemical, biological, and structural properties. The current review discusses the recent progress, challenges, and future perspectives of electrospun polysaccharides in periodontal tissue engineering.

Crystallization and preliminary X-ray analysis of S-ribosylhomocysteinase from Streptococcus mutans.

S-Ribosylhomocysteinase (LuxS) encoded by the luxS gene from Streptococcus mutans plays a crucial role in the quorum-sensing system. LuxS was solubly expressed in Escherichia coli with high yield. The purity of the purified target protein, which was identified by SDS-PAGE and MALDI-TOF MS analysis, was >95%. The protein was crystallized using the hanging-drop vapour-diffusion method with PEG 3350 as the primary precipitant. X-ray diffraction data were collected at Beijing Synchrotron Radiation Facility (BSRF). Diffraction by the crystal extended to 2.4 Å resolution and the crystal belonged to space group C222(1), with unit-cell parameters a = 55.3, b = 148.7, c = 82.8 Å.