General Control Non-derepressible 2 Alleviates Cartilage Degeneration and Inhibits NLRP3 Inflammasome Activation in Osteoarthritis.

This study aimed to evaluate the effects of general control non-derepressible 2 (GCN2) on osteoarthritis (OA) in vivo and in vitro. First, anterior cruciate ligament transection (ACLT)-induced rat model and interleukin (IL)-1ß-induced ATDC5 chondrocyte were established. Hematoxylin and eosin staining and safranin O/fast green staining were employed for analyzing the histological changes in the rat cartilage. In addition, immunohistochemistry, quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay, western blot, and immunofluorescence staining were employed for examining cartilage degeneration-, inflammation-, autophagy-, and NLR family pyrin domain containing 3 (NLRP3) inflammasome-associated genes expression. Moreover, 2,7-dichlorodihydrofluorescein acetoacetic acid probe was utilized for examining the intracellular reactive oxygen species. In addition, 5-ethynyl-2'-deoxyuridine assay and flow cytometry were applied for detecting chondrocyte proliferation and apoptosis IL-1ß-treated ATDC5 chondrocytes. GCN2 overexpression ameliorated articular cartilage degeneration and inflammation but promoted chondrocyte autophagy in ACLT-induced OA rats. Similarly, we demonstrated that the upregulation of GCN2 could promote chondrocyte proliferation, suppress chondrocyte apoptosis, attenuate chondrocyte inflammation and extracellular matrix degradation, and promote chondrocyte autophagy. Moreover, GCN2 overexpression could inhibit the activation of NLRP3 inflammasome in IL-1ß-induced ATDC5 chondrocyte. Furthermore, 3-methyladenine neutralized the protective and autophagy-promoting effects of GCN2 overexpression on ATDC5 chondrocytes. GCN2 could attenuate inflammation and cartilage degeneration, promote chondrocyte autophagy, and inhibit NLRP3 inflammasome activation in OA.

Inverse relationship between femoral lateralization and neck-shaft angle is a joint event after intramedullary nailing of per trochanteric fractures.

This study explored the relationship between femoral lateralization and femoral neck-shaft angle after intramedullary nail (IM) fixation for per trochanteric fractures. 70 patients (AO/OTA 31A1-2) were investigated. Anteroposterior (AP) and lateral X-ray views pre- and post-operation were recorded. Patients were classified into three groups according to the position of the medial cortex of the head-neck fragment to that of the femoral shaft: being slightly superomedial (positive medial cortex support, PMCS), being smoothly contacted (neutral position, NP) or being displaced laterally (negative medial cortex support, NMCS). Patient demographics, femoral lateralization, and neck-shaft angle were measured and statistically analyzed pre- and post-operation. Functional recovery was evaluated by Harris score 3- and 6- months post-operation. All cases ultimately demonstrated radiographic fracture union. There was a tendency to have an increased neck-shaft angle (valgus alignment) in the PMCS group and increased femoral lateralization in the NP group (p < 0.05). Among those three groups, the change in femoral lateralization and neck-shaft angle was statistically different (p < 0.05). An inverse relationship between femoral lateralization and femoral neck-shaft angle was observed. Femoral lateralization increased correspondingly when the neck-shaft angle continuously decreased from the PMCS group to the NP group and then to the NMCS group, and patients in the PMCS group had better functional recovery than the other two groups (p < 0.05). Femoral lateralization was commonly produced after IM fixation for per trochanteric fractures. The fracture fixed in PMCS mode possesses the slightest change in femoral lateralization while maintaining valgus alignment of the femoral neck-shaft angle and good functional outcome, which is superior to NP or NMCS mode.

Quantifying CO2 Removal at Enhanced Weathering Sites: a Multiproxy Approach.

Enhanced weathering is a carbon dioxide (CO2) mitigation strategy that promises large scale atmospheric CO2 removal. The main challenge associated with enhanced weathering is monitoring, reporting, and verifying (MRV) the amount of carbon removed as a result of enhanced weathering reactions. Here, we study a CO2 mineralization site in Consett, Co. Durham, UK, where steel slags have been weathered in a landscaped deposit for over 40 years. We provide new radiocarbon, δ13C, 87Sr/86Sr, and major element data in waters, calcite precipitates, and soils to quantify the rate of carbon removal. We demonstrate that measuring the radiocarbon activity of CaCO3 deposited in waters draining the slag deposit provides a robust constraint on the carbon source being sequestered (80% from the atmosphere, 2σ = 8%) and use downstream alkalinity measurements to determine the proportion of carbon exported to the ocean. The main phases dissolving in the slag are hydroxide minerals (e.g., portlandite) with minor contributions (<3%) from silicate minerals. We propose a novel method for quantifying carbon removal rates at enhanced weathering sites, which is a function of the radiocarbon-apportioned sources of carbon being sequestered, and the proportion of carbon being exported from the catchment to the oceans.

Environmental and Mineralogical Controls on Biosignature Preservation in Magnesium Carbonate Systems Analogous to Jezero Crater, Mars.

Jezero Crater on Mars is a paleolacustrine environment where Mg-carbonates may host evidence of ancient life. To elucidate the environmental and mineralogical controls on biosignature preservation, we examined samples from five terrestrial analogs: Lake Salda (Turkey), Lake Alchichica (Mexico), Qinghai-Tibetan Plateau (China), Mg-carbonate playas (British Columbia, Canada), and a mine with fine-grained ultramafic tailings (Yukon, Canada). The mineralogical compositions of the samples varied, yet were often dominated by either aragonite (CaCO3) or hydromagnesite [Mg5(CO3)4(OH)2·4H2O]. Aragonite-rich samples from Alchichica, Mg-carbonate playas, and the ultramafic mine contained an abundance of entombed microbial biomass, including organic structures that resembled cells, whereas hydromagnesite-rich samples were devoid of microfossils. Aragonite often precipitates subaqueously where microbes thrive, thereby increasing the likelihood of biomass entombment, while hydrated Mg-carbonates typically form by evaporation in subaerial settings where biofilms are less prolific. Magnesite (MgCO3), the most stable Mg-carbonate, forms extremely slowly, which may limit the capture of biosignatures. Hydrated Mg-carbonates are prone to transformation via coupled dissolution-precipitation reactions that may expose biosignatures to degradation. Although less abundant, aragonite is commonly found in Mg-carbonate environments and is a better medium for biosignature preservation due to its fast precipitation rates and relative stability, as well as its tendency to form subaqueously and lithify. Consequently, we propose that aragonite be considered a valuable exploration target on Mars.

A novel lotus seed cross-linked resistant starch: Structural, physicochemical and digestive properties.

The structural properties and physicochemical characteristics of lotus seed cross-linked resistant starches (LSCSs; LS-0CS, LS-1CS, LS-2CS, LS-4CS, LS-6CS, LS-8CS, LS-10CS, and LS-12CS) with different concentrations of cross-linking agents were investigated. The degrees of cross-linking of LSCSs increased along with the amount of cross-linking agent. The higher the degree of cross-linking, the greater the degree of LSCSs granule agglomeration. The occurrence of the cross-linking reaction was confirmed by the appearance of P = O at 1,250 cm-1 as assessed by FT-IR, and the covalent bonds formed by the phosphate group in LSCSs were mainly composed of distarch monophosphate (DMSP) as determined by 31P NMR. As the crosslinking degree increased, the peak strength of DMSP in starch was stronger and the specific gravity of DMSP was larger. Among the samples, LS-12CS had the highest cross-linking degree, with a greater specific gravity of DMSP. Moreover, the solubility levels of LSCSs decreased and the thermal stability and anti-digestive properties improved as the cross-linking degree increased, which was correlated with the degree of agglomeration and DMSP in LSCSs. The RS content of LS-12CS was 48.95 ± 0.86%.

Properties and digestibility of a novel porous starch from lotus seed prepared via synergistic enzymatic treatment.

The objective was to investigate the effect of synergistic enzymatic treatment on the properties and digestibility of a novel C-type lotus seed porous starch (LPS). Scanning electron microscopy showed that the densest and most complete pores were formed on the surface of LPS when the concentration of enzymes added was 1.5% (LS-1.5E). With increases in enzyme addition, the oil and water absorption of the porous starch increased and reached maxima at 1.5% of enzyme. Increased in the specific surface area, total pore volume and average pore diameter of LPS were determined by low-temperature nitrogen adsorption, while when the enzymes exceeded 1.5%, there were no significant changes. Compared to lotus seed starch (LS), the particle size of LPS also decreased. With the increases in enzyme addition, LPS exhibited higher relative crystallinity and ordering structure by XRD and FTIR. The results by SAXS confirmed that LPS had higher ordered semi-crystalline lamellar and denser lamellar structure compared to LS. Low-field 1H NMR spectroscopy indicated that the proportion of bound water in LPS increased, while the proportion of bulk water decreased. Moreover, the degree of hydrolysis of LPS was lower than that of LS, and the content of rapidly digestible starch decreased, while the content of slowly digestible starch and resistant starch increased with the enzyme addition, which was consistent with the structural properties.

Synergistic Effects of Lotus Seed Resistant Starch and Sodium Lactate on Hypolipidemic Function and Serum Nontargeted Metabolites in Hyperlipidemic Rats.

The synergistic effects of lotus seed resistant starch (LRS3) and sodium lactate (SL; a postbiotics of RS3) on hypolipidemic function and serum nontargeted metabolites of hyperlipidemia rats were investegated. Rats fed a high-fat diet were orally administered with LRS3 (HLRS group) or SL (HSL group) either alone or in combination (HLRSSL group) for consecutive 4 weeks. HLRSSL was found to control weight gain, regulate blood lipid levels, reduce accumulation of fat in liver cells, and improve lesions in rat cardiac arteries, liver, small intestine, and colon tissues more effectively compared to HLRS or HSL group alone. Compared to the high-fat control group (HMC), l-phenylalanine and LysoPC(22:6(4Z,7Z,10Z,13Z,16Z,19Z)) in serum were upregulated in HLRSSL rats, while aconitic acid and suberic acid were decreased. Correlation analysis showed that SM(d18:0/16:1(9Z)), taurochenodeoxycholic acid, LysoPC(22:6(4Z,7Z,10Z,13Z,16Z,19Z)), oleic acid, and retinol were negatively correlated with total cholesterol (TCHO), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) and positively correlated with high-density lipoprotein cholesterol (HDL-C). Moreover, glutamic acid and serine showed a significant positive correlation with LDL-C and negative correlation with HDL-C. These differential metabolites were associated with reducing serum lipid levels in hyperlipidemia rats potentially through metabolic pathways such as linoleic acid, glutamine and glutamate, pyruvate, citric acid cycle, and glycerophospholipid.

Reverse wedge effect following intramedullary nail fixation of trochanteric fracture, what does it imply?

Lag screw cut-out is the most common cause of fixation failure of trochanteric fractures. Intraoperative assessment of fracture reduction and fixation quality is vital to avoid fracture reduction and achieve good functional outcomes. In a recent study, Zhang et al. reported the occurrence of a reverse wedge effect after intraoperative nail insertion based on a new computed tomography(CT)-guided fracture classification system, which specifically happened to the basicervical facture type and resulted in valgus deformity with gapping at the medial inferior fracture line. Impingement between the reamer/nail and superolateral cortex of the femoral neck has been regarded as the main cause. Based on these findings, together with an extensive literature review, the practicality of the new fracture classification system, the definition of basicervical trochanteric fracture, and the mechanisms underlying the reverse wedge effect have been deeply discussed. More studies should be carried out in the future to analyse pre- and intraoperative related factors that could affect the intraoperative fragment migration effects and determine highly specific measures to address them.

Multi-Stage Pedestrian Positioning Using Filtered WiFi Scanner Data in an Urban Road Environment.

Since widespread applications of wireless sensors networks, low-speed traffic positioning based on the received signal strength indicator (RSSI) from personal devices with WiFi broadcasts has attracted considerable attention. This study presents a new range-based localization method for outdoor pedestrian positioning by using the combination of offline RSSI distance estimation and real-time continuous position fitting, which can achieve high-position accuracy in the urban road environment. At the offline stage, the piecewise polynomial regression model (PPRM) is proposed to formulate the Euclidean distance between the targets and WiFi scanners by replacing the common propagation model (PM). The online stage includes three procedures. Firstly, a constant velocity Kalman filter (CVKF) is developed to smooth the real-time RSSI time series and estimate the target-detector distance. Then, a least squares Taylor series expansion (LS-TSE) is developed to calculate the actual 2-dimensional coordinate with the replacement of existing trilateral localization. Thirdly, a trajectory-based technique of the unscented Kalman filter (UKF) is introduced to smooth estimated positioning points. In tests that used field scenarios from Guangzhou, China, the experiments demonstrate that the combined CVKF and PPRM can achieve the highly accurate distance estimator of <1.98 m error with the probability of 90% or larger, which outperforms the existing propagation model. In addition, the online method can achieve average positioning error of 1.67 m with the much better than classical methods.

Growth of Au Hollow Stars and Harmonic Excitation Energy Transfer.

Optical excitation, subsequent energy transfer, and emission are fundamental to many physical problems. Optical antennas are ideal candidates for manipulating these processes. We extend energy transfer to second- and third-harmonic (SH and TH) fields through the collaborative susceptibility χ(n) (n = 1, 2, 3) resonances of nonlinear optical antennas. Hollow gold stars, with a broadband response covering the fundamental, SH, and TH frequencies, are synthesized as nonlinear antennas. Harmonic resonance energy transfer through a χ(3) → χ(1) collaboration is revealed. A χ(3) → χ(2) collaboration is uncovered, with largely enhanced SH radiation demonstrated by exciting the three resonances at the fundamental, SH, and TH frequencies. A theoretical model of the effective nonlinear susceptibilities is proposed to calculate the efficiencies of the two nonlinear energy transfer processes.

[Prospective clinical study on extracorporeal shock wave therapy combined with platelet-rich plasma injection for knee osteoarthritis].

OBJECTIVE: To investigate the effectiveness of extracorporeal shock wave therapy combined with platelet-rich plasma (PRP) injection in treatment of knee osteoarthritis (KOA) by prospective clinical study. METHODS: Between June 2015 and June 2018, 180 patients with KOA met the inclusion criteria were included in study and randomly allocated to group A (n=60), group B (n=60), and group C (n=60). The patients were treated with autologous PRP intra-articular injection in group A, extracorporeal shock wave therapy in group B, and extracorporeal shock wave therapy combined with autologous PRP intra-articular injection in group C, once a week and 5 times a duration of treatment. There was no significant difference in age, gender, disease duration, side of KOA, and Kellgren-Lawrence grading between groups (P>0.05). The pain and function of knee joint were assessed by visual analogue scale (VAS) score, Lequesne Index score, Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score, and knee joint activity before treatment and at 1, 3, and 5 weeks after the first treatment. RESULTS: There were significant differences in VAS score, Lequesne Index score, WOMAC score, and knee joint activity between pre- and post-treatment in all groups (P<0.05). VAS score, Lequesne Index score, and WOMAC score gradually decreased with the prolongation of treatment time (P<0.05); but there was no significant difference in knee joint activity between different time points (P>0.05). There was no significant difference in VAS score, Lequesne Index score, WOMAC score, and knee joint activity between groups before treatment (P>0.05); the scores of group C were superior to groups A and B (P< 0.05) at different time points after treatment; while the knee joint activities of 3 groups were similar (P>0.05). CONCLUSION: The extracorporeal shock wave therapy combined with PRP injection can relieve the pain synergistically for KOA.

A Pt-free pristine monolithic carbon aerogel counter electrode for dye-sensitized solar cells: up to 20% under dim light illumination.

In this work, pristine carbon aerogels (CAs) were used as Pt-free counter electrodes (CEs) in dye-sensitized solar cells (DSSCs) by varying the molar ratio of their precursors. Pristine mesoporous CAs with controlled resorcinol (R)/formaldehyde (F) and resorcinol (R)/sodium carbonate (C) molar ratios were successfully prepared. The as-prepared CAs were synthesized via a polymeric sol-gel reaction and were labeled as CA-O, CA-Q, CA-F, CA-C, and CA-G. The DSSCs using the as-prepared CA-C CE gave the best power conversion efficiency (PCE, η), 9.08 ± 0.01%, among all the CA CEs. The CA-C CE is further applied to an indoor T5 light source system with an impressive η value of 20.1 ± 0.60% at 2.18 mW cm-2 (T5 lamp with 7000 lux). Moreover, the hardness of CA-C CE is 3.01 GPa (Brinell hardness test), which is comparable to that of the FTO/glass substrate. As a result, the CA-C CE shows great potential to replace traditional CEs based on the Pt/FTO/glass in DSSCs.

Highly tunable nonlinear response of Au@WS2 hybrids with plasmon resonance and anti-Stokes effect.

We synthesize Au@WS2 hybrid nanobelts and investigate their third-order nonlinear responses mediated by a strong anti-Stokes effect. By using the femtosecond Z-scan technique and tuning the excitation photon energy (Eexc), we find the sign reversals of both nonlinear absorption coefficient ß and nonlinear refractive index γ to be around 1.60 eV, which is prominently higher than the bandgap (1.35 eV) of WS2 bulk owing to the strong anti-Stokes processes around the bandgap of the indirect semiconductors. The saturable absorption and self-defocusing of the WS2 nanobelts are significantly enhanced by the plasmon resonance of the Au nanoparticles when Eexc > 1.60 eV. But the excited state absorption assisted by the anti-Stokes processes and the self-focusing observed at Eexc < 1.60 eV are suppressed by the surface plasmon. Furthermore, by using population rate equations, we theoretically analyze the sign reversals of both ß and γ and reveal the physical mechanism of the unique nonlinear responses of the hybrids with the plasmon resonance and anti-Stokes effect. These observations enrich the understanding of the nonlinear processes and interactions between the plasmon and exciton and are helpful for developing nonlinear optical nanodevices.

Rehabilitation treatment of spastic cerebral palsy with radial extracorporeal shock wave therapy and rehabilitation therapy.

This aims to investigate the effect of combined use of radial extracorporeal shock wave therapy (rESWT) and conventional rehabilitation therapy on postoperative rehabilitation of children with spastic cerebral palsy.Children with spastic cerebral palsy 6 weeks after multistage surgery were randomly divided into treatment group (received rESWT and conventional rehabilitation therapy) and control group (received conventional rehabilitation only). Before treatment, 2 weeks and 1 month after treatment, the Gross Motor Function Measure (GMFM), modified Ashworth Scale (MAS) of the hamstrings and triceps, plantar area and plantar pressure were examined for efficacy assessment.A total of 82 children with spastic cerebral palsy were recruited, including 43 children in treatment group and 39 children in control group. There was no significant difference in the age, MAS score, and GMFM score between the 2 groups before treatment. There were statistically significant differences between the 2 groups at 2 weeks and 4 weeks after treatment, including the MAS score, GMFM score, plantar area and plantar pressure (P < .05). Within groups, there were also significant differences at different times (P < .05).The rESWT combined with rehabilitation can quickly and effectively relieve paralysis of lower extremities, reduce the tension of hamstrings and calf muscles, relieve muscle spasm, and rapidly improve limb function in children with spastic cerebral palsy.

Largely enhanced photocatalytic hydrogen production rate of CdS/(Au-ReS2) nanospheres by the dielectric-plasmon hybrid antenna effect.

In this study, we synthesized CdS/(Au-ReS2) nanospheres that have highly efficient photocatalytic hydrogen production activity induced by dielectric-plasmon hybrid antenna resonance. As the diameter (D) of ReS2 nanospheres consisting of 2D nanosheets increases from 114 ± 11 to 218 ± 25 nm, the resonance wavelength of the ReS2 dielectric antenna is tuned from 380 to 620 nm and the hydrogen production rate for the CdS/(Au-ReS2) nanospheres increases by more than 1.85 times and reaches a value as high as 3060 µmol g-1 h-1, with a 9% weight percentage of Au. Due to the enhancements of the local electromagnetic field and excitation energy transfer by the ReS2-Au dielectric-plasmon hybrid antenna, the hydrogen production rate for the CdS/(Au-ReS2) nanospheres (D = 218 ± 25 nm) is 797, 319, 105 and 12 times larger than that for pure ReS2, Au-ReS2, CdS, and CdS-ReS2, respectively. Additionally, the persistence and reusability measurements indicate a favorable stability of CdS/(Au-ReS2). These results provide a strategy to prepare a new class of dielectric-plasmon hybrid antennas consisting of 2D materials and metal nanoparticles, which have promise in applications ranging from photocatalysis to nonlinear optics.

A Highly Stable Framework of Crystalline Zinc Phosphite with Selective Removal, Recovery, and Turn-On Sensing Abilities for Mercury Cations in Aqueous Solutions.

A highly stable framework of organic-inorganic hybrid zinc phosphite (NTOU-4) and its cobalt analogue (NTOU-4a) were synthesized under the hydro(solvo)thermal conditions and structurally characterized by single-crystal X-ray diffraction. Their frameworks consisted of inorganic metallophosphite chains, in which the metal atoms were interlinked through 1H-1,2,4-triazole-3,5-diamine and 1,4-benzenedicarboxylate linkers to form new crystalline materials. It is extremely difficult to achieve the consolidation of three distinct coordinations of metal-carboxylate, metal-triazolate, and metal-phosphite bonds into one crystal, resulting in the synthesis of the first mixed-ligand terephthalate-metallophosphite solids in the absence of organic molecules as templates or space-filling counters in their structures. Interestingly, the zinc compound not only exhibits high thermal stability (up to 400 °C in air) and chemical resistance to seawater, aqueous solutions (pH 3-11), and organic solvents at boiling conditions, but also shows selective removal, recovery, and "turn-on" sensing abilities of toxic mercury ions in aqueous solutions. Furthermore, the synthesis, characterization, and the difference of the framework stabilities between isostructural zinc and cobalt compounds are also reported.

A Series of Organic-Inorganic Hybrid Zinc Phosphites Containing Extra-Large Channels.

A series of organic-inorganic hybrid zinc phosphites with extra-large channels were synthesized and characterized by single-crystal X-ray diffraction. This is an unusual example of introducing 1,4-benzenedicarboxylate and/or biphenyl-4,4'-dicarboxylate ligands into the organically templated metal phosphite system to build extra-large-channel zeolite-related materials via hydro(solvo)thermal reactions. Those frameworks are composed of carboxylate linkers and inorganic tubes of zinc phosphites, translating their channel windows from a square shape (NTOU-1) to rhombus forms (NTOU-2 and NTOU-3) via the replacement of organic amines or ligands under synthesis conditions otherwise identical with those used to prepare NTOU-1. The synthesis, structural diversity, photoluminescence, and adsorption properties for dye molecules and lanthanide ions are also reported.

Controlled growth and optical response of a semi-hollow plasmonic nanocavity and ultrathin sulfide nanosheets on Au/Ag platelets.

Herein, we report a strategy to construct a semi-hollow plasmonic nanocavity and grow ultrathin sulfide nanosheets inside. The competition and cooperation of Au deposition with Ag etching based on flat Ag nanoplates are proposed. For the establishment of the semi-hollow nanocavity, Au shells are grown on Ag nanoplates, which serve as a stable frame, followed by partial etching of the Ag nanoplates. By controlling the thickness of the initial Ag nanoplates or the injected amount of etchant, the nanocavity size is fine-tuned. Significantly, the remaining unetched Ag layers provide a flat platform for the growth of 2D ultrathin sulfides of Ag2S and CdS inside the semi-hollow plasmonic nanocavity. Strong plasmon resonance and large local field enhancement are exhibited inside the plasmonic cavity where the ultrathin semiconductor sulfides are grown, indicating strong plasmon-exciton interactions in the hybrids. Furthermore, this synthetic approach is extended to grow other metal sulfides such as Bi2S3 and PbS. The combination of a flat plasmonic cavity with ultrathin semiconductor nanosheets in this study provides a new strategy for the development of unique plasmon-based hybrids with excellent optical properties.

The nonmonotonous shift of quantum plasmon resonance and plasmon-enhanced photocatalytic activity of gold nanoparticles.

The surface plasmon resonance (SPR) of metal nanoparticles exhibits quantum behaviors as the size decreases owing to the transitions of quantized conduction electrons, but most studies are limited to the monotonous SPR blue-shift caused by off-resonant transitions. Here, we demonstrate the nonmonotonous SPR red-shift caused by resonant electron transitions and photocatalytic activity enhanced by the quantum plasmon resonance of colloidal gold nanoparticles. A maximal SPR wavelength and the largest photocatalytic activity are observed in the quantum regime for the first time for the gold nanoparticles with a diameter of 3.6 nm. Theoretical analysis based on a quantum-corrected model reveals the evolution of SPR with quantized electron transitions and well explains the nonmonotonous size-dependencies of the SPR wavelength and absorption efficiency. These findings have profound implications for the understanding of the quantum nature of the SPR of metal nanoparticles and their applications in areas ranging from photophysics to photochemistry.

Efficacy and safety evaluation of intra-articular injection of tranexamic acid in total knee arthroplasty operation with temporarily drainage close.

OBJECTIVE: To investigate the efficacy and safety of tranexamic acid (TXA) injection during primary total knee arthroplasty (TKA) for reducing postoperative hemorrhage. METHODS: 100 cases of patients admitted to our hospital and underwent primary unilateral TKA from January 2012 to December 2014 were enrolled in this study and they were divided randomly into two groups. For the TXA group, 1 g TXA was dissolved in 50 ml 0.9% sodium chloride solution and injected after prosthesis implantation but before cavity close. Conventional drainage clamping was carried for 4 h and the drainage tube was removed 48 h postoperative. For the control group, similar measures were taken except for that no TXA was dissolved in 0.9% sodium chloride solution. Postoperative hemoglobin, blood coagulation index, total blood loss volume, drainage volume, blood transfusion rate and lower extremity deep vein thrombosis (DVT) rate in both groups were observed and the efficacy and safety of this surgical treatment were evaluated. RESULTS: There were no significant differences in operation time, postoperative platelet and APPT, D-dimer, lower limb venous thrombosis incidence rate 1 week after operation between the two groups. Postoperative drainage volume, hemoglobin, total blood loss and blood transfusion rate in the TXA group were significantly lower than those of the control group. Ecchymosis of lower extremity peripheral incision and its surroundings was significantly milder than that of the control group. CONCLUSION: Intraoperative intra-articular injection of TXA in TKA can significantly reduce the initial postoperative hemorrhage and blood transfusion rate at the early stage after operation.