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Braz J Microbiol ; 51(1): 87-94, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31667800

RESUMO

NADPH oxidases are enzymes that have been reported to generate reactive oxygen species (ROS) in animals, plants and many multicellular fungi in response to environmental stresses. Six genes of the NADPH oxidase complex components, including vvnoxa, vvnoxb, vvnoxr, vvbema, vvrac1 and vvcdc24, were identified based on the complete genomic sequence of the edible fungus Volvariella volvacea. The number of vvnoxa, vvrac1, vvbema and vvcdc24 transcripts fluctuated with ageing, and the gene expression patterns of vvnoxa, vvrac1 and vvbema were significantly positively correlated. However, the expression of vvnoxb and vvnoxr showed no significant difference during ageing. In hyphae subjected to mechanical injury stress, both O2- and H2O2 concentrations were increased. The expression of vvnoxa, vvrac1, vvbema and vvcdc24 was substantially upregulated, but vvnoxb and vvnoxr showed no response to mechanical injury stress at the transcriptional level. Additionally, the transcription of vvnoxa, vvrac1, vvbema and vvcdc24 could be repressed when the intracellular ROS were eliminated by diphenyleneiodonium (DPI) chloride and reduced glutathione (GSH) treatments. These results indicated a positive feedback loop involving NADPH oxidase and intracellular ROS, which might be the reason for the oxidative burst during injury stress.


Assuntos
Regulação Fúngica da Expressão Gênica , Micélio/genética , NADPH Oxidases/genética , Volvariella/enzimologia , Volvariella/genética , Proteínas Fúngicas/genética , Genoma Fúngico , Glutationa/farmacologia , Micélio/enzimologia , Oniocompostos/farmacologia , Oxirredução , Espécies Reativas de Oxigênio/metabolismo , Explosão Respiratória , Estresse Fisiológico
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