Micro-Particle Image Velocimetry (microPIV): recent developments, applications, and guidelines.

In this review we discuss the state of the art of the optical whole-field velocity measurement technique micro-scale Particle Image Velocimetry (microPIV). microPIV is a useful tool for fundamental research of microfluidics as well as for the detailed characterization and optimization of microfluidic applications in life science, lab-on-a-chip, biomedical research, micro chemical engineering, analytical chemistry and other related fields of research. An in depth description of the microPIV method is presented and compared to other flow visualization and measurement methods. An overview of the most relevant applications is given on the topics of near-wall flow, electrokinetic flow, biological flow, mixing, two-phase flow, turbulence transition and complex fluid dynamic problems. Current trends and applications are critically reviewed. Guidelines for the implementation and application are also discussed.

Tapered microfluidic chip for the study of biochemical and mechanical response at subcellular level of endothelial cells to shear flow.

A lab-on-a-chip application for the investigation of biochemical and mechanical response of individual endothelial cells to different fluid dynamical conditions is presented. A microfluidic flow chamber design with a tapered geometry that creates a pre-defined, homogeneous shear stress gradient on the cell layer is described and characterized. A non-intrusive, non-tactile measurement method based on micro-PIV is used for the determination of the topography and shear stress distribution over individual cells with subcellular resolution. The cellular gene expression is measured simultaneously with the shape and shear stress distribution of the cell. With this set-up the response of the cells on different pre-defined shear stress levels is investigated without the influence of variations in repetitive experiments. Results are shown on cultured endothelial cells related to the promoter activity of the shear-responsive transcription factor KLF2 driving the marker gene for green fluorescent protein.

Nanoparticle image velocimetry at topologically structured surfaces.

Nanoparticle image velocimetry (nano-PIV), based on total internal reflection fluorescent microscopy, is very useful to investigate fluid flows within approximately 100 nm from a surface; but so far it has only been applied to flow over smooth surfaces. Here we show that it can also be applied to flow over a topologically structured surface, provided that the surface structures can be carefully configured not to disrupt the evanescent-wave illumination. We apply nano-PIV to quantify the flow velocity distribution over a polydimethylsiloxane surface, with a periodic gratinglike structure (with 215 nm height and 2 mum period) fabricated using our customized multilevel lithography method. The measured tracer displacement data are in good agreement with the computed theoretical values. These results demonstrate new possibilities to study the interactions between fluid flow and topologically structured surfaces.

The endothelin-1 pathway and the development of cardiovascular defects in the haemodynamically challenged chicken embryo.

BACKGROUND/AIMS: Ligating the right lateral vitelline vein of chicken embryos (venous clip) results in cardiovascular malformations. These abnormalities are similar to malformations observed in knockout mice studies of components of the endothelin-1 (ET-1)/endothelin-converting enzyme-1/endothelin-A receptor pathway. In previous studies we demonstrated that cardiac ET-1 expression is decreased 3 h after clipping, and ventricular diastolic filling is disturbed after 2 days. Therefore, we hypothesise that ET-1-related processes are involved in the development of functional and morphological cardiovascular defects after venous clip. METHODS: In this study, ET-1 and endothelin receptor antagonists (BQ-123, BQ-788 and PD145065) were infused into the HH18 embryonic circulation. Immediate haemodynamic effects on the embryonic heart and extra-embryonic vitelline veins were examined by Doppler and micro-particle image velocimetry. Ventricular diastolic filling characteristics were studied at HH24, followed by cardiovascular morphologic investigation (HH35). RESULTS: ET-1 and its receptor antagonists induced haemodynamic effects at HH18. At HH24, a reduced diastolic ventricular passive filling component was demonstrated, which was compensated by an increased active filling component. Thinner ventricular myocardium was shown in 42% of experimental embryos. CONCLUSION: We conclude that cardiovascular malformations after venous clipping arise from a combination of haemodynamic changes and altered gene expression patterns and levels, including those of the endothelin pathway.

In vivo micro particle image velocimetry measurements of blood-plasma in the embryonic avian heart.

The measurement of blood-plasma velocity distributions with spatial and temporal resolution in vivo is inevitable for the determination of shear stress distributions in complex geometries at unsteady flow conditions like in the beating heart. A non-intrusive, whole-field velocity measurement technique is required that is capable of measuring instantaneous flow fields at sub-millimeter scales in highly unsteady flows. Micro particle image velocimetry (muPIV) meets these demands, but requires special consideration and methodologies in order to be utilized for in vivo studies in medical and biological research. We adapt muPIV to measure the blood-plasma velocity in the beating heart of a chicken embryo. In the current work, bio-inert, fluorescent liposomes with a nominal diameter of 400 nm are added to the flow as a tracer. Because of their small dimension and neutral buoyancy the liposomes closely follow the movement of the blood-plasma and allow the determination of the velocity gradient close to the wall. The measurements quantitatively resolve the velocity distribution in the developing ventricle and atrium of the embryo at nine different stages within the cardiac cycle. Up to 400 velocity vectors per measurement give detailed insight into the fluid dynamics of the primitive beating heart. A rapid peristaltic contraction accelerates the flow to peak velocities of 26 mm/s, with the velocity distribution showing a distinct asymmetrical profile in the highly curved section of the outflow tract. In relation to earlier published gene-expression experiments, the results underline the significance of fluid forces for embryonic cardiogenesis. In general, the measurements demonstrate that muPIV has the potential to develop into a general tool for instationary flow conditions in complex flow geometries encountered in cardiovascular research.