RESUMO
After vascular angioplasty, vascular smooth muscle cell (VSMC) proliferation causes atherosclerosis and intimal hyperplasia leading to restenosis. Interferon-γ-inducible protein (IP)-10 plays a role in atherogenesis, but the mechanism remains unclear. We evaluated the role of IP-10 in intimal hyperplasia and restenosis. IP-10 expression was determined in arterial specimens from 20 arteriosclerotic obliteration patients and 6 healthy individuals. VSMCs were stimulated in vitro with IFN-γ and transfected with IP-10 siRNA. Silencing was verified with RT-PCR/Western blot; cell proliferation rate was detected by methyl-thiazol-tetrazolium. The carotid artery model of atherosclerosis injury was established with IP-10 siRNA. IP-10 expression was detected at 1 and 4 weeks using RT-PCR and immunohistochemistry. Artery morphology was assessed with hematoxylin-and-eosin staining, and intimal hyperplasia was evaluated by electron microscopy. IP-10 was overexpressed in arteriosclerotic obliteration group compared with control group (P < 0.05). IP-10 expression in transfected group was significantly lower than in untransfected group. The intima-to-media ratio of transfected group at 4 weeks was lower than that of untransfected group (P < 0.01). The transfected group exhibited more regular intimal structure and less hyperplasia under electron microscopy. We, therefore, concluded that IP-10 played an important role in intimal hyperplasia as siRNA-mediated IP-10 silencing inhibited aberrant VSMCs hyperplasia and reduced restenosis.
Assuntos
Artéria Carótida Primitiva/patologia , Quimiocina CXCL10/antagonistas & inibidores , Reestenose Coronária/prevenção & controle , Músculo Liso Vascular/metabolismo , Interferência de RNA , Idoso , Angioplastia Coronária com Balão , Animais , Proliferação de Células , Células Cultivadas , Quimiocina CXCL10/genética , Quimiocina CXCL10/metabolismo , Reestenose Coronária/patologia , Modelos Animais de Doenças , Feminino , Humanos , Hiperplasia , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Músculo Liso Vascular/citologia , RNA Interferente Pequeno/metabolismo , Coelhos , Túnica Íntima/metabolismo , Túnica Íntima/patologiaRESUMO
OBJECTIVE@#To investigate the expression of Calponin-1 and Transgenlin in the uterine smooth muscles during normal labor on-sets, and to evaluate their effect on initiating the normal labor.@*METHODS@#A total of 14 uterine bodies and lower segments of human pregnancy were divided to a non-labor group (NIL) and a labor group(IL). Immunohistochemical technology and Western blot were used to determine the expression of Calponin-1 and Transgelin in the 2 groups.@*RESULTS@#Immunohistochemical detection and Western blot showed that Calponin-1 protein in the uterine smooth muscle tissue of the body and the lower uterine segment of smooth muscle tissues had significant difference (P0.05).@*CONCLUSION@#Calponin-1 of the uterine smooth muscle and Transgelin of the uterine body smooth muscle may involve in the regulation of uterine smooth muscle contractility, which is closely related to child birth launch.
Assuntos
Adulto , Feminino , Humanos , Gravidez , Proteínas de Ligação ao Cálcio , Genética , Metabolismo , Início do Trabalho de Parto , Metabolismo , Proteínas dos Microfilamentos , Genética , Metabolismo , Proteínas Musculares , Genética , Metabolismo , Miométrio , Metabolismo , Contração Uterina , MetabolismoRESUMO
Objective To investigate the effect of Calponin-1 suppression on human myometrium cells through adenovirus mediated siRNA. Methods Human uterine smooth muscle tissues were digested with enzymes, cultured and confirmed with immunocytochemistry. Aadenovirus siRNA-Calponin-1 plasmid was transfected into primary cultured uterine smooth muscle cells in vitro. The expressions of Calponin-1 mRNA and protein were analyzed by RT-PCR and Western blot, respectively.Results The pAdEasy-pShuttle-U6-Calponin-1 siRNA plasmid was successfully constructed, and Calponin-1 siRNA mediated by recombinant adenovirus resulted in markedly reduced expression of Calponin-1 mRNA and protein in human myometrium cells. The gray values of Calponin-1 mRNA in the uterine smooth muscle cells in the experimental, blank control, and empty vector groups were 316.3±39.2, 1048.5±126.4 and 1027.2±127.5, respectively. The gray values of Calponin-1 protein were 323.3±43.2, 1021.5±143.4, and 1019.2±144.5,respectively. The difference between the experimental group and the blank control group as well as the empty vector group was significant (P< 0.05). There was no significant difference between the empty vector group and the blank control group (P>0.05).Conclusion The pAdEasy-pShuttle-U6-Calponin-1 siRNA plasmid can inhibit the expression of Calponin-1 in human myometrium cells in vitro,which may be a useful approach to determine the role of Calponin-1 in delivery.
RESUMO
Objective To explore the effects of passive smoking on pregnant rats and embryo development.Methods The pregnant Wistar rats were exposed to passive smoking4times per day,10min per time,continuously for20days,and the body weights were measured at the1st day,10th day and20th day.At the at20th day,the concentrations of NO in serum of pregnant rats were determined,simultansly the fetal death,embrgonic absorption were observed,the body weights,body length,tail length,brain weight and live weight of fetuses fetal growth retardation(IUGR)were observed.Results Significantly higher frequencies of fetal death,embryonic absorption,IUGR and significantly lower concentration s of NO in serum of pregnant rats were found in experiment group exposed to passive smoking.Significantly lower levels of body weight,body length,tail length,brain weight of fetuses were found in experiment group exposed to passive smoking compared with those of controls(P
