RESUMO
Neurological symptoms have been associated with Leishmania infection, however little is known about how the nervous system is affected in leishmaniasis. This work aimed to analyze parasitic load, production of cytokines/neurotrophins in the prefrontal cortex and behavioral changes in BALB/c mice infected with Leishmania amazonensis. At 2 and 4months post-infection, infected mice showed a decrease in IFN-γ, IL-1, IL-6, IL-4, IL-10 cytokines and BDNF and NGF neurotrophins in prefrontal cortex associated with increased anxiety behavior. Parasite DNA was found in brain of all animals at 4months post-infection, when the levels of IBA-1 (activated macrophage/microglia marker) and TNF-α was increased in the prefrontal cortex. However TNF-α returned to normal levels at 6months post-infection suggesting a neuroprotective mechanism.
Assuntos
Córtex Cerebral/metabolismo , Citocinas/metabolismo , Leishmaniose/complicações , Leishmaniose/patologia , Transtornos Mentais/etiologia , Fatores de Crescimento Neural/metabolismo , Animais , Proteínas de Ligação ao Cálcio/genética , Proteínas de Ligação ao Cálcio/metabolismo , Córtex Cerebral/parasitologia , DNA de Protozoário/genética , DNA de Protozoário/metabolismo , Modelos Animais de Doenças , Comportamento Exploratório , Regulação da Expressão Gênica , Leishmania mexicana/genética , Leishmania mexicana/patogenicidade , Leishmaniose/microbiologia , Masculino , Aprendizagem em Labirinto/fisiologia , Transtornos Mentais/parasitologia , Camundongos , Camundongos Endogâmicos BALB C , Proteínas dos Microfilamentos/genética , Proteínas dos Microfilamentos/metabolismo , Pele/patologia , Fatores de TempoRESUMO
This work was conducted on rats in two premises located in Niterói and Rio de Janeiro, Brazil. One is classified as conventional controlled and the other, conventional. The objective of the present study was to detect the presence of Mycoplasma pulmonis in animals with symptoms of respiratory disease and low reproductive performance. In the conventional controlled premises, 16 rats of Wistar-Furth strain were necropsied while in the conventional premises necropsy was performed on 12 rats of Hooded Lister strain. The clinical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturing while the sera were tested for antibody detection. From 28 rats, 57.14 percent (16/28) were culture positive for M. pulmonis, being 81.25 percent (13/16) from the conventional controlled premises, and 25.00 percent (3/12) from the conventional premises. The ELISA test was carried out in 20 animals of both colonies. In the conventional controlled premises, 92.86 percent (13/14) were positive for M. pulmonis, and 7.14 percent (1/14) were suspicious, while in the conventional premises, 100 percent (6/6) of the samples were positive. The results confirmed that M. pulmonis was the etiologic agent of the disease that affected the rats under study, and that the ELISA positivity rated higher than culture
