RESUMO
The article analyses the process of establishment of medical knowledge concerning territories of the Russian Empire in XVIII century on the basis of written heritage of the Prussian physician Johann Jacob Lerche (1708-1780). The analysis is focused on forms of actual medical geographical studies practiced by European doctors and Lerche himself, first of all on his meteorological observations. The Lerche's interpretations of local natural and social phenomena in construction of medical characteristics of particular corners of the Empire in manuscript about endemics in Russia and Persia are analyzed. The assumption is proposed that studies of Russia implemented by Lerche are constructed on the analogy of summarizing academic descriptions of naturalists of XVIII century. Thereby, the works of Prussian physician seems to be participated in process of development of language of systematization and ranking of diseases and endowed category of "disease" with function of inherent to region characteristic such as climate, flora and fauna.
Assuntos
Médicos , Geografia Médica , Humanos , Federação Russa/epidemiologiaRESUMO
A trial of inactivated hepatitis A viral vaccine of heteroploid cell culture origin is described. The vaccine preparation was tested in guinea pigs and tamarins. The animals were immunized intramuscularly four or three times, respectively. The efficacy was judged by induction of anti-HAV antibody persisting for at least 12 months in guinea pigs, and development of immunity to subsequent virus challenge (monkeys only). The challenge dose of HAV was unable to produce any signs of HAV infection in the vaccinated tamarins, although the booster effect was observed in some animals. The study demonstrated that the tested batches of the vaccine were highly immunogenic.
Assuntos
Hepatovirus/imunologia , Vacinas contra Hepatite Viral/imunologia , Animais , Anticorpos Antivirais/sangue , Avaliação Pré-Clínica de Medicamentos , Cobaias , Hepatite A/prevenção & controle , Imunização , Técnicas Imunoenzimáticas , Imunoglobulina M/análise , Isocitrato Desidrogenase/sangue , Masculino , Saguinus , Fatores de Tempo , Vacinas de Produtos Inativados/imunologiaRESUMO
According to the WHO requirements, the concentration of cellular DNA in vaccine preparations produced by pooling virus from continuous cell lines is limited to 100 ng/dose. In this study, different methods were used for purification of tick-borne encephalitis virus suspensions grown in continuous cultures of cell line 4647 from cellular DNA. Two approaches are proposed based on treatment with DNAse and promamin sulfate which allow one to reduce cellular DNA concentration in the virus preparation to the acceptable level. Prospects of their use in vaccine production are discussed.
