Variation in reproductive mode across the latitudinal range of invasive Russian knapweed.

For invading species, reproduction is a critical determinant of population establishment as well as spread into new areas. When species have multiple modes of reproduction, the prevalence of different modes can influence management decisions. We used genetic markers to determine the prevalent method of recruitment for invasive Russian knapweed (Acroptilon repens). This species forms patches and can spread by both rhizomic growth and seed from outcrossing. We found no shared genotypes between 41 western North American populations, indicating at the macroscale, Russian knapweed is spreading via seed to distant locations. We also examined drivers of reproductive mode by comparing clonality with large-scale environmental factors across the invasion. We found a correlation between latitude and clonal versus seed reproduction, with clonality higher in northern latitude populations. This trend was associated most parsimoniously with decreasing maximum annual temperature and 30-year average of available growing degree days, and increasing soil organic carbon content. These results have management implications: if not properly temporally implemented, grazing or herbicide applications that create open spaces for recruitment may increase the likelihood of Russian knapweed patch persistence through seed, and recently released galling biological control agents in North America may be less effective in northern latitudes where Russian knapweed spread by seed is less prevalent.

The effects of agent hybridization on the efficacy of biological control of tansy ragwort at high elevations.

The success rate of weed biological control programs is difficult to evaluate and the factors affecting it remain poorly understood. One aspect which is still unclear is whether releases of multiple, genetically distinct populations of a biological control agent increase the likelihood of success, either by independent colonization of different environmental niches or by hybridization that may increase the agent's fitness and adaptive ability. Since hybridization is often invoked to explain the success of unintentionally introduced exotic species, hybridization among biocontrol agents may be similarly important in shaping the effectiveness of biological control programs. In this study, we first evaluated intraspecific hybridization among populations of a weed biological control agent, the ragwort flea beetle, Longitarsus jacobaeae. These insects were introduced as part of a classical biological control program from Italy and Switzerland. We genotyped 204 individuals from 15 field sites collected in northwest Montana, and an additional 52 individuals that served as references for Italian and Swiss populations. Bayesian analysis of population structure assigned seven populations as pure Swiss and one population as pure Italian, while intraspecific hybrid individuals were detected in seven populations at frequencies of 5%-69%. Subsequently, we conducted a 2-year exclusion experiment using six sites with Swiss beetles and three with hybrid beetles to evaluate the impact of biological control. We found that biological control by Swiss beetles and by hybrid beetles is effective, increasing mortality of the target plant, Jacobaea vulgaris, by 42% and 45%, and reducing fecundity of surviving plants by 44% and 72%, respectively. Beetle densities were higher and mortality of larger plants was higher at sites with hybrids present. These results suggest that hybridization of ragwort flea beetles at high-elevation sites may improve biological control of tansy ragwort and that intraspecific hybridization of agents could benefit biological control programs.