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2.
Ukr Biokhim Zh (1978) ; 53(5): 49-54, 1981.
Artigo em Russo | MEDLINE | ID: mdl-7292621

RESUMO

The phosphorylation reaction of lysyl-tRNA-synthetase from the rat liver tissue was studied in the in vivo experiments. The activity of 3':5'-AMP-dependent protein kinase from the myocardium in the phosphorylation reaction is 3.4 times lower than that of the enzyme from the liver. Optimum conditions of the lysyl-tRNA-synthetase phosphorylation reaction were determined. The Vmax and Km values for the reaction were 2.7 pmol of 32P/mg per 1 minute and 0.4-10(-4), respectively. The lysyl-tRNA-synthetase phosphorylation increases the tRNA aminoacylation.


Assuntos
Aminoacil-tRNA Sintetases/metabolismo , Fígado/enzimologia , Lisina-tRNA Ligase/metabolismo , Animais , Cinética , Miocárdio/enzimologia , Fosforilação , Proteínas Quinases/metabolismo , Ratos
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