Tobacco smoke exposure and mitochondrial DNA copy number on neurobehavioural performance: A community study.

The influence of tobacco smoke has been a controversial and very questionable subject within the field of neurological behaviours. To examine the dose-response relationships between tobacco smoke and neurological performance, we investigated whether mitochondrial DNA copy number (mtDNAcn) mediates these relationships. We used restricted cubic spline models to estimate the dose-response relationships. A mediation model was also used to detect the mediating effect. Increased cotinine was negatively associated with auditory memory scores and a 0.51 decrease in mtDNAcn. MtDNAcn acts as a mediator between cotinine and auditory memory. Tobacco smoke levels were inversely associated with mtDNAcn and neurobehavioural changes, and there was a mediation effect between cotinine levels and auditory memory by mtDNAcn.

Physicochemical, antioxidant properties of giant croaker (Nibea japonica) swim bladders collagen and wound healing evaluation.

Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were obtained from Nibea japonica swim bladders. The denaturation temperature (Td) of ASC and PSC was approximately 33.8 °C. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared spectroscopy (FTIR) analyses indicated that ASC and PSC contained triple-helical type I collagen when compared to rat tail collagen type I. Moreover, the microstructure of collagen sponges was uniform and porous. In addition, ASC and PSC exhibited antioxidant properties and in vitro scratch assays showed that PSC at various concentrations (0, 12.5, 25, and 50 µg/mL) had significant effects on the scratch closure rate. Furthermore, collagen sponge from Nibea japonica swim bladders exhibited an increased efficacy of wound healing when compared to the control mice. The levels of interleukin (IL)-1ß, IL-6 and tumor necrosis factor (TNF)-α in the collagen sponge treated mice were significantly decreased when compared to the control group. Thus, our results suggested that collagen sponge from Nibea japonica swim bladders has potential wound healing applications.

Collagen Extracted from Bigeye Tuna (Thunnus obesus) Skin by Isoelectric Precipitation: Physicochemical Properties, Proliferation, and Migration Activities.

Collagen was extracted from bigeye tuna (Thunnus obesus) skins by salting-out (PSC-SO) and isoelectric precipitation (PSC-IP) methods. The yield of the PSC-IP product was approximately 17.17% (dry weight), which was greater than the yield obtained from PSC-SO (14.14% dry weight). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicated that collagen from bigeye tuna skin belongs to collagen type I. Inductively coupled plasma mass spectrometry results indicate that the heavy metal abundance in PSC-IP was lower than the maximum acceptable amounts according to Chinese regulatory standards. In addition, results from a methylthiazolyldiphenyl-tetrazolium bromide assay and an in vitro scratch assay demonstrated that PSC-IP could promote the proliferation and migration of NIH-3T3 fibroblasts. Overall, results suggest PSC-IP could be used to rapidly extract collagen from marine by-products instead of traditional salting-out methods. Collagen from bigeye tuna skin may also have strong potential for cosmetic and biomedical applications.

On the Stability of NaO2 in Na-O2 Batteries.

Na-O2 batteries are regarded as promising candidates for energy storage. They have higher energy efficiency, rate capability, and chemical reversibility than Li-O2 batteries; in addition, sodium is cheaper and more abundant compared to lithium. However, inconsistent observations and instability of discharge products have inhibited the understanding of the working mechanism of this technology. In this work, we have investigated a number of factors that influence the stability of the discharge products. By means of in operando powder X-ray diffraction study, the influence of oxygen, sodium anode, salt, solvent, and carbon cathode were investigated. The Na metal anode and an ether-based solvent are the main factors that lead to the instability and decomposition of NaO2 in the cell environment. This fundamental insight brings new information on the working mechanism of Na-O2 batteries.

Towards an Understanding of Li2 O2 Evolution in Li-O2 Batteries: An In†Operando Synchrotron X-ray Diffraction Study.

One of the major challenges in developing high-performance Li-O2 batteries is to understand the Li2 O2 formation and decomposition during battery cycling. In this study, this issue was investigated by synchrotron radiation powder X-ray diffraction. The evolution of Li2 O2 morphology and structure was observed under actual electrochemical conditions of battery operation. By quantitatively tracking Li2 O2 during discharge and charge, a two-step process was suggested for both growth and oxidation of Li2 O2 owing to different mechanisms during two stages of both oxygen reduction reaction and oxygen evolution reaction. From an observation of the anisotropic broadening of Li2 O2 in XRD patterns, it was inferred that disc-like Li2 O2 grains are formed rapidly in the first step of discharge. These grains can stack together so that they facilitate the nucleation and growth of toroidal Li2 O2 particles with a LiO2 -like surface, which could cause parasitic reactions and hinder the formation of Li2 O2 . During the charge process, Li2 O2 is firstly oxidized from the surface, followed by a delithiation process with a faster oxidation of the bulk by stripping the interlayer Li atoms to form an off-stoichiometric intermediate. This fundamental insight brings new information on the working mechanism of Li-O2 batteries.