A taurine-based hydrogel with the neuroprotective effect and the ability to promote neural stem cell proliferation.

Ischemic stroke, a cerebrovascular disease caused by arterial occlusion in the brain, can lead to brain impairment and even death. Stem cell therapies have shown positive advantages to treat ischemic stroke because of their extended time window, but the cell viability is poor when transplanted into the brain directly. Therefore, a new hydrogel GelMA-T was developed by introducing taurine on GelMA to transplant neural stem cells. The GelMA-T displayed the desired photocuring ability, micropore structure, and cytocompatibility. Its compressive modulus was more similar to neural tissue compared to that of GelMA. The GelMA-T could protect SH-SY5Y cells from injury induced by OGD/R. Furthermore, the NE-4C cells showed better proliferation performance in GelMA-T than that in GelMA during both 2D and 3D cultures. All results demonstrate that GelMA-T possesses a neuroprotective effect for ischemia/reperfusion injury against ischemic stroke and plays a positive role in promoting NSC proliferation. The novel hydrogel is anticipated to function as cell vehicles for the transplantation of neural stem cells into the stroke cavity, aiming to treat ischemic stroke.

Optimizing the Production of Hydrogel Microspheres Using Microfluidic Chips: The Influence of Surface Treatment on Droplet Formation Mechanism.

Microfluidic chips have been widely applied in biology and medical research for stably generating uniform droplets that can be solidified into hydrogel microspheres. However, issues such as low microsphere yield, lengthy experimental processes, and susceptibility to environmental interference need to be addressed. In this work, a simple and effective method was developed to modify microfluidic chips at room temperature to improve the production performance of hydrogel microspheres. Numerical simulation-assisted experiments were conducted to comprehensively understand the effect of solution viscosity, hydrophilicity, and flow rate ratio on droplet formation during microsphere production. Chitosan was selected as the main component and combined with poly(ethylene glycol) diacrylate to prepare photocurable hydrogel microspheres as a demonstration. As a result, grafting fluoro-silane (FOTS) increased the contact angle of the channel from 90 to approximately 110°, which led to a 12.2% increase in droplet yield. Additionally, FOTS-modification attenuated the impact of the flow rate ratio on droplet yield by 19.1%. Alternatively, depositing dopamine decreased the channel's contact angle from 90 to 60°, resulting in a 21.4% increase in particle size and enabling the chip to adjust droplet size over a wider range. Further study demonstrates that the obtained hydrogel microspheres can be modified with layers of aldehyde, which can potentially be used for controlled drug release. Overall, this study proposed a facile method for adjusting the yield and droplet size through surface treatment of microfluidic chips while also enhancing the understanding of the synergistic effects of multiple factors in microfluidics-based microsphere production.

Two-step method fabricating a 3D nerve cell model with brain-like mechanical properties and tunable porosity vascular structures via coaxial printing.

Three-dimensional (3D) nerve cell models have been widely developed to understand the mechanisms and discover treatment methods of ischemic stroke and neurodegenerative disease. However, there is a contradiction in the production of 3D models that they should possess high modulus to ensure mechanical stability while low modulus to provide mechanical stimuli for nerve cells. In addition, it is challenging to maintain the long-term viability of 3D models when lacking vascular structures. Here, a 3D nerve cell model with brain-like mechanical properties and tunable porosity vascular structures has been fabricated. The matrix materials with brain-like low mechanical properties were favorable for promoting HT22 proliferation. The nerve cells could exchange nutrients and waste with the cultural environment through vascular structures. The vascular structures also played a supporting role, and model stability was enhanced by combining matrix materials with vascular structures. Furthermore, the porosity of vascular structure walls was adjusted by adding sacrificial materials to the tube walls during 3D coaxial printing and removing them after preparation, resulting in tunable porosity vascular structures. Finally, HT22 cells showed better cell viability and proliferation performance after culturing 7 days in the 3D models with vascular structures than in the 3D models with solid structures. All these results suggest that this 3D nerve cell model possesses good mechanical stability and long-term viability, which is expected to be used in pathological studies and drug screening for ischemic stroke and neurodegenerative diseases.

Ferroferric oxide loaded near-infrared triggered photothermal microneedle patch for controlled drug release.

HYPOTHESIS: The drug release efficiency of microneedle is usually slower than that of oral delivery or hypodermic injection, which severely restricts its widespread use. Herein, a Fe3O4-loaded photothermal microneedle (Fe3O4@MN) patch is developed for controlled drug delivery. Under near infrared (NIR) irradiation, the drug loaded on Fe3O4@MN can be quickly released, achieving an enhanced drug release efficiency. EXPERIMENTS: The mechanical property and characterization of Fe3O4@MN were systematically investigated, and the photothermal performance of Fe3O4@MN was also conducted. Moreover, the model-drug-releasing tests and doxycycline hydrochloride releasing tests were carried out to evaluate the drug release performance of Fe3O4@MN under NIR irradiation. FINDINGS: Fe3O4@MN has enough mechanical strength to pierce into skins, and the temperature of Fe3O4@MN patch could rapidly increase by 40 â„ƒ in 1 min under NIR irradiation. In vitro experiment, the release rate of model drug in Fe3O4@MN reached âˆ¼ 80 % in 20 min and the doxycycline hydrochloride release rate of Fe3O4@MN reached âˆ¼ 70 % after 20 min of NIR irradiation, indicating the potential application of the synthesized microneedle patch for transdermal drug delivery. Further penetration test showed that the penetration depth of model drugs carried by Fe3O4@MN patch on the porcine skin under NIR irradiation was 150 - 200 µm longer than that of the patch without Fe3O4 nanoparticles.

Probing the Self-Assembly and Nonlinear Friction Behavior of Confined Gold Nano-Particles.

For the wide application of nanoparticles (NPs) (e.g., in nanotribology), it is of fundamental and practical importance to understand the self-assembly and lubrication behavior of confined NPs. In this work, a systematic study was conducted to probe the assembly and associated surface forces of spherical gold nanoparticles (Au NPs, diameter ∼5 nm) confined between pairs of mica (negatively charged) and (3-aminopropyl)triethoxysilane modified mica (APTES-mica, positively charged) surfaces using a surface forces apparatus (SFA) under aqueous conditions. It is observed that Au NPs were squeezed out of the confined gap between two mica surfaces during the loading process, resulting from the repulsive electric-double layer force. In contrast, multilayers of Au NPs were confined between two APTES-mica surfaces because of the attractive double-layer force between oppositely charged Au NPs and APTES-mica. Interestingly, the interaction between Au NPs and APTES-mica is stronger than the interactions between Au NPs, resulting in the rearrangement of the confined Au NPs under shearing. Importantly, a large friction coefficient (µ > 0.7) with unexpected nonlinear stick-slip friction was observed when sliding two APTES-mica surfaces with thin layers of Au NPs (∼20 nm) confined in between. The observed stick-slip motion could be explained by the velocity-dependent friction model where a critical shear velocity was required for transiting from stick-slip to smooth sliding. Our study provides useful information on the assembly and interaction forces of confined nanoparticles on charged surfaces, with implications for predicting the behaviors of NPs under confinement in various engineering applications.