Fiber-Optic Probe Array for Measuring Spatial Distributions of Air Volume Fractions in Bubbly Flows.

In this study, we developed a fiber-optic sensing system with an eight-probe array for measuring the spatial distributions of air volume (void) fractions in bubbly flows. Initially, we performed calibration experiments in a cylindrical tank by using a fiber-optic sensing system with a single probe to determine the relationship between the time fraction ratio of bubble signals and void fractions. A high correlation coefficient was obtained between the time fraction ratio and the void fraction, suggesting that the proposed fiber-optic sensing system can measure local void fractions of up to 18%. Subsequently, we used the proposed fiber-optic sensing system with the eight-probe array to measure the spatial distribution of air volume fractions in a bubbly flow caused by breaking waves near a submerged breakwater. The effects of different variables, including the incident wave height, period, and width of the breakwater, on the spatial distribution of the void fraction on the lee side of the breakwater were systematically studied. The results demonstrated that the proposed fiber-optic sensing system can be used to determine the spatial distribution of air volume fractions in bubbly flows.

AuAg nanocomposites suppress biofilm-induced inflammation in human osteoblasts.

Staphylococcus aureus (S. aureus)forms biofilm that causes periprosthetic joint infections and osteomyelitis (OM) which are the intractable health problems in clinics. The silver-containing nanoparticles (AgNPs) are antibacterial nanomaterials with less cytotoxicity than the classic Ag compounds. Likewise, gold nanoparticles (AuNPs) have also been demonstrated as excellent nanomaterials for medical applications. Previous studies have showed that both AgNPs and AuNPs have anti-microbial or anti-inflammatory properties. We have developed a novel green chemistry that could generate the AuAg nanocomposites, through the reduction of tannic acid (TNA). The bioactivity of the nanocomposites was investigated inS. aureusbiofilm-exposed human osteoblast cells (hFOB1.19). The current synthesis method is a simple, low-cost, eco-friendly, and green chemistry approach. Our results showed that the AuAg nanocomposites were biocompatible with low cell toxicity, and did not induce cell apoptosis nor necrosis in hFOB1.19 cells. Moreover, AuAg nanocomposites could effectively inhibited the accumulation of reactive oxygen species (ROS) in mitochondria and in rest of cellular compartments after exposing to bacterial biofilm (by reducing 0.78, 0.77-fold in the cell and mitochondria, respectively). AuAg nanocomposites also suppressed ROS-triggered inflammatory protein expression via MAPKs and Akt pathways. The current data suggest that AuAg nanocomposites have the potential to be a good therapeutic agent in treating inflammation in bacteria-infected bone diseases.

Development and Applications of a Pressurized Water-Filled Impedance Tube.

In this study, a pressurized, water-filled impedance tube (WFIT) was developed to measure the reflection coefficients of sound-absorbing materials under various hydrostatic pressures. The developed WFIT was calibrated using a two-microphone, three-parameter calibration method (3PCM). The accuracy and repeatability of the measured reflection coefficients for the water-air interface in the WFIT were determined by comparing these coefficients with corresponding theoretical reflection coefficients. The WFIT was then used to measure the acoustic reflection coefficient of a porous rubber specimen on three dates, and the corresponding measurement results exhibited satisfactory repeatability. The aforementioned impedance tube was also used to measure the reflection coefficient of a porous rubber specimen under a hydrostatic pressure of 4 Patm three times on the same day, and one time each on three days, using the same experimental setup and measurement procedure. The results obtained in the aforementioned tests also exhibited satisfactory repeatability. Finally, the WFIT was used to measure the reflection coefficients of porous rubber specimens with various thicknesses under different hydrostatic pressures. The results of this study indicate that the developed WFIT calibrated with the 3PCM can achieve suitable repeatability in the measurement of the reflection coefficients of sound-absorbing materials under various hydrostatic pressures.

Underwater Sound Source Localization Based on Passive Time-Reversal Mirror and Ray Theory.

This study investigates the performance of a passive time-reversal mirror (TRM) combined with acoustic ray theory in localizing underwater sound sources with high frequencies (3-7 kHz). The TRM was installed on a floating buoy and comprised four hydrophones. The ray-tracing code BELLHOP was used to determine the transfer function between a sound source and a field point. The transfer function in the frequency domain obtained from BELLHOP was transformed into the time domain. The pressure field was then obtained by taking the convolution of the transfer function in the time domain with the time-reversed signals that were received by the hydrophones in the TRM. The location with the maximum pressure value was designated as the location of the source. The performance of the proposed methodology for source localization was tested in a towing tank and in the ocean. The aforementioned tests revealed that even when the distances between a source and the TRM were up to 1600 m, the distance deviations between estimated and actual source locations were mostly less than 2 m. Errors originated mainly from inaccurate depth estimation, and the literature indicates that they can be reduced by increasing the number of TRM elements and their apertures.