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Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 28(7): 702-5, 2012 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-22768859

RESUMO

AIM: To observe the effects of megsin gene transfection on the expressions of monocyte chemoattractant protein-1 (MCP-1) and intercellular adhesion molecule-1(ICAM-1). METHODS: Mouse glomerular mesangial cells were cultured in high glucose medium, and then cell proliferation was measured by MTT assay at 12, 24 and 48 h respectively. The expressions of megsin, MCP-1 and ICAM-1 in mesangial cells were detected by immunocytochemical staining and Western blotting. The concentration of type IV collagen in the culture supernatant of mesangial cells was measured by ELISA. RESULTS: Under high glucose concentration, the expressions of megsin, MCP-1 and ICAM-1 increased; the concentration of type IV collagen in the cell supernatant was elevated as well; and mesangial cell proliferation was enhanced. After megsin gene transfection, the above changes were more significant, but were abated following megsin shRNA/transfection. CONCLUSION: Megsin gene can up-regulate the expressions of MCP-1 and ICAM-1, promote mesangial cell proliferation and mesangial extracellular matrix accumulation.


Assuntos
Quimiocina CCL2/metabolismo , Regulação da Expressão Gênica , Molécula 1 de Adesão Intercelular/metabolismo , Células Mesangiais/metabolismo , Serpinas/genética , Serpinas/metabolismo , Animais , Linhagem Celular , Proliferação de Células , Colágeno Tipo IV/metabolismo , Mesângio Glomerular/metabolismo , Camundongos , Transfecção
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