RESUMO
Twenty-seven microsatellite markers recommended by the Food and Agriculture Organization (FAO) and the International Society for Animal Genetics (ISAG) were used to analyze the genetic diversities of 7 Chinese native miniature pig breeds/strains (Jiuyang Xiang Pig, Jianbai Xiang Pig, Congjiang Xiang Pig, Huanjiang Xiang Pig, Black Xiang Pig, Wuzhishan Pig, and Diannan Small Ear Pig) and 3 western pig breeds (Duroc, Large White, and Landrace). The results indicate that there exists, in general, a high degree of genetic variability within the 7 miniature pig breeds. However, the genetic variability in Jiuyang Xiang Pig, Jianbai Xiang Pig, Huanjiang Xiang Pig, and Congjiang Xiang Pig (average PICs being 0.61-0.64) was significantly lower than that in the other three miniature pig breeds (average PICs being 0.60-0.84). The genetic divergence among Jiuyang, Jianbai, Huanjiang, and Congjiang was very small (Nei's standard genetic distances being 0.12-0.22), but it was large between them and the other three miniature pig breeds (Nei's standard genetic distances being 1.61-1.96), and even larger between them and the three foreign breeds (Nei's standard genetic distances being 1.99-3.30). Cluster analysis reveals three obvious clusters for the 10 breeds: Jiuyang, Jianbai, Huanjiang, and Congjiang are in one cluster, the other three miniature pig breeds in the other, and the three foreign breeds in the third. These results are consistent with the geography distribution and origin of these breeds.
Assuntos
Variação Genética , Repetições de Microssatélites/genética , Sus scrofa/genética , Animais , Cruzamento , Análise por Conglomerados , Feminino , Masculino , Filogenia , Entorses e Distensões , Sus scrofa/classificação , SuínosRESUMO
The primers, RF/RR and QF1/QR,were designed based on the homologous region of swine MHC-DQB and DRB exon1 sequences and HLA-DQB and HLA-DRB sequences. The 5' proximal promoter region (URR) nucleotide sequences of swine MHC-DQB and DRB were firstly obtained by PCR, cloning and sequencing techniques. There exist highly conserved sequence motifs including W, X, Y, CCAAT, and TATA-like boxes in the obtained sequences,showing the same organization of the conserved regulatory elements as in other species. 12 DRB-URR and 14 DQB-URR alleles were found by PCR-SSCP in 313 pigs. The aligned results of these alleles showed that there exist abundant polymorphism sites in both Porcine MHC-DQB and DRB proximal promoter region.
Assuntos
Antígenos de Histocompatibilidade Classe II/genética , Polimorfismo Genético , Regiões Promotoras Genéticas/genética , Suínos/genética , Alelos , Animais , Sequência de Bases , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Polimorfismo Conformacional de Fita Simples , Elementos Reguladores de Transcrição , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico , TATA BoxRESUMO
RNA was extracted from spleens of diarrhea and non-diarrhea piglets of Jiuyang Pig, Jianbai Pig and Landrace. RNA pools were established and DDRT-PCR using three anchored and seven arbitrary primers combined with silver staining was conducted to identify ESTs differentially expressed in diarrhea resistance to E. coli K88. Five cDNA were identified in the non-diarrhea RNA pools. Among them two are new sequences and three are highly identical to the ESTs in the GeneBank, of which two have known functions. One is the mammalian Nck adaptor protein 1 and the other is the human LINE-1 reverse transcriptase.
