RESUMO
Gut microbes in insects may play an important role in the digestion, immunity and protection, detoxification of toxins, development, and reproduction. The rice leaffolder Cnaphalocrocis medinalis (Guenée) (Lepidoptera: Crambidae) is a notorious insect pest that can damage rice, maize, and other gramineous plants. To determine the effects of host plants and generations on the gut microbiota of C. medinalis, we deciphered the bacterial configuration of this insect pest fed rice or maize for three generations by Illumina MiSeq technology. A total of 16 bacterial phyla, 34 classes, 50 orders, 101 families, 158 genera, and 44 species were identified in C. medinalis fed rice or maize for three generations. Host plants, insect generation, and their interaction did not influence the alpha diversity indices of the gut microbiota of C. medinalis. The dominant bacterial taxa were Proteobacteria and Firmicutes at the phylum level and Enterococcus and unclassified Enterobacteriaceae at the genus level. A number of twenty genera coexisted in the guts of C. medinalis fed rice or maize for three generations, and their relative abundances occupied more than 90% of the gut microbiota of C. medinalis. A number of two genera were stably found in the gut of rice-feeding C. medinalis but unstably found in the gut microbiota of maize-feeding C. medinalis, and seven genera were stably found in the gut of maize-feeding C. medinalis but unstably found in the gut of rice-feeding C. medinalis. In addition, many kinds of microbes were found in some but not all samples of the gut of C. medinalis fed on a particular host plant. PerMANOVA indicated that the gut bacteria of C. medinalis could be significantly affected by the host plant and host plant × generation. We identified 47 taxa as the biomarkers for the gut microbiota of C. medinalis fed different host plants by LEfSe. Functional prediction suggested that the most dominant role of the gut microbiota in C. medinalis is metabolism, followed by environmental information processing, cellular processes, and genetic information processing. Our findings will enrich the understanding of gut bacteria in C. medinalis and reveal the differences in gut microbiota in C. medinalis fed on different host plants for three generations.
RESUMO
Introduction: The insect gut harbors numerous microorganisms that may have functions in development and reproduction, digestion, immunity and protection, and detoxification. Recently, the influence factors on gut microbiota were evaluated in the rice leaffolder Cnaphalocrocis medinalis, a widespread insect pest in paddy fields. However, the relationship between gut microbiota composition and geography is poorly understood in C. medinalis. Methods: To reveal the patterns of C. medinalis gut bacterial communities across geographic sources and the ecological processes driving the patterns, C. medinalis were sampled from six geographic sources in China, Thailand, and Vietnam in 2016, followed by gut bacterial 16S ribosomal RNA gene sequencing. Results: A total of 22 bacterial phyla, 56 classes, 84 orders, 138 families, 228 genera, and 299 species were generated in C. medinalis from six geographic sources. All alpha diversity indices differed among the samples from different geographic sources. Analysis of similarity (ANOSIM) and permutational multivariate analysis of variance (PERMANOVA) both revealed significant differences in the gut microbiota of C. medinalis from six geographic sources. A total of 94 different taxa were screened as indicators for the gut microbiota of C. medinalis from six geographic sources by linear discriminant analysis effect size (LEfSe). The gene ontology (GO) pathways of the gut microbiota in C. medinalis differed among geographic sources. In total, the bacterial communities within geographic sources were mainly determined by stochastic processes, and those between geographic sources were mainly determined by deterministic processes. Discussion: This study elucidates that geography plays a crucial role in shaping the gut microbiota of C. medinalis. Thus, it enriches our knowledge of gut bacteria in C. medinalis and sheds light on the mechanisms underlying C. medinalis gut microbial shifts across geography.
RESUMO
Cotesia ruficrus (Haliday), a gregarious larval endoparasitoid, is an important biological control agent of various pest species. The developmental interactions between the host rice leaf folder, Cnaphalocrocis medinalis (Guenée), and its koinobiont parasitoid, C. ruficrus, were investigated for the first time under laboratory conditions. The effects of host instar at parasitization on the development time, clutch size, and survival of C. ruficrus were determined. The results showed that the parasitoids starting parasitism in the fourth-instar larvae had the shortest development duration and highest fecundity. Meanwhile, the growth of the host parasitized by C. ruficrus in various instars was also observed. The results indicated that the growth of the parasitized larvae was significantly inhibited, compared with unparasitized ones, irrespective of the host instar at oviposition. In addition, the effect of parasitism on food consumption and utilization of the fourth-instar larvae was determined, suggesting that the nutritional physiology of the host was affected by parasitism. Wet or dry weight gain, food consumption, and fecal matter were all significantly reduced in the parasitized larvae in contrast with the unparasitized larvae. Parasitization by C. ruficrus could significantly increase the approximate digestibility of the host.
