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1.
Stem Cell Res Ther ; 15(1): 214, 2024 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-39020385

RESUMO

Sciatic nerve injury (SNI) is a common type of peripheral nerve injury typically resulting from trauma, such as contusion, sharp force injuries, drug injections, pelvic fractures, or hip dislocations. It leads to both sensory and motor dysfunctions, characterized by pain, numbness, loss of sensation, muscle atrophy, reduced muscle tone, and limb paralysis. These symptoms can significantly diminish a patient's quality of life. Following SNI, Wallerian degeneration occurs, which activates various signaling pathways, inflammatory factors, and epigenetic regulators. Despite the availability of several surgical and nonsurgical treatments, their effectiveness remains suboptimal. Exosomes are extracellular vesicles with diameters ranging from 30 to 150 nm, originating from the endoplasmic reticulum. They play a crucial role in facilitating intercellular communication and have emerged as highly promising vehicles for drug delivery. Increasing evidence supports the significant potential of exosomes in repairing SNI. This review delves into the pathological progression of SNI, techniques for generating exosomes, the molecular mechanisms behind SNI recovery with exosomes, the effectiveness of combining exosomes with other approaches for SNI repair, and the changes and future outlook for utilizing exosomes in SNI recovery.


Assuntos
Exossomos , Nervo Isquiático , Exossomos/metabolismo , Exossomos/transplante , Humanos , Animais , Nervo Isquiático/lesões , Traumatismos dos Nervos Periféricos/terapia , Traumatismos dos Nervos Periféricos/metabolismo , Regeneração Nervosa
2.
Heliyon ; 10(8): e29158, 2024 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-38644876

RESUMO

Objective: To establish a predictive modeling for the risk of bloodstream infection associated with peripherally inserted central catheter (PICC). Methods: Patients receiving PICC treatment in Shenzhen People's Hospital from June 2020 to December 2020 were retrospectively enrolled and divided into the infection group and the non-infection group according to the presence and absence of PICC-related infections. Then, relevant clinical information of patients was collected and the predictors of PICC-related infection were screened by the least absolute shrinkage and selection operator regression (LASSO) model. Besides, multivariate logistic regression was used to analyze the influencing factors of PICC-related infection, A nomogram was constructed based on the results of the multivariate analysis. Ultimately, a receiver operating characteristic (ROC) curve was plotted to analyze the application value of influencing factors to predict PICC-related infections. Results: A total of 505 patients were included, including 75 patients with PICC-related infections (14.85%). The main pathogen was gram-positive cocci. The predictors screened by LASSO included age >60 years, catheter movement, catheter maintenance cycle, insertion technique, immune function, complications, and body temperature ≥37.2 °C before PICC placement. Multivariate logistic regression analysis showed that independent risk factors of infections related to PICC included age >60 years [odds ratio (OR) = 1.722; 95% confidence interval (CI) = 1.312-3.579; P = 0.006], catheter movement (OR = 1.313; 95% CI = 1.119-3.240; P = 0.014), catheter maintenance cycle >7 days (OR = 2.199; 95% CI = 1.677-4.653; P = 0.000), direct insertion (OR = 1.036; 95% CI = 1.019-2.743; P = 0.000), poor immune function (OR = 2.322; 95% CI = 2.012-4.579; P = 0.000), complications (OR = 1.611; 95% CI = 1.133-3.454; P = 0.019), and body temperature ≥37.2 °C before PICC placement (OR = 1.713; 95% CI = 1.172-3.654; P = 0.012). Besides, the area under the ROC curve was 0.889. Conclusion: PICC-related infections are associated with factors such as age >60 years, catheter movement, catheter maintenance cycle, insertion technique, immune function, complications, and body temperature ≥37.2 °C before PICC placement. Additionally, the LASSO model is moderately predictive for predicting the occurrence of PICC-related infections.

3.
Forensic Sci Int ; 357: 111974, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38447346

RESUMO

Afghanistan and Myanmar are two overwhelming opium production places. In this study, rapid and efficient methods for distinguishing opium from Afghanistan and Myanmar were developed using infrared spectroscopy (IR) coupled with multiple machine learning (ML) methods for the first time. A total of 146 authentic opium samples were analyzed by mid-IR (MIR) and near-IR (NIR), within them 116 were used for model training and 30 were used for model validation. Six ML methods, including partial least squares discriminant analysis (PLS-DA), orthogonal PLS-DA (OPLS-DA), k-nearest neighbour (KNN), support vector machine (SVM), random forest (RF), and artificial neural networks (ANNs) were constructed and compared to get the best classification effect. For MIR data, the average of precision, recall and f1-score for all classification models were 1.0. For NIR data, the average of precision, recall and f1-score for different classification models ranged from 0.90 to 0.94. The comparison results of six ML models for MIR and NIR data showed that MIR was more suitable for opium geography classification. Compared with traditional chromatography and mass spectrometry profiling methods, the advantages of MIR are simple, rapid, cost-effective, and environmentally friendly. The developed IR chemical profiling methodology may find wide application in classification of opium from Afghanistan and Myanmar, and also to differentiate them from opium originating from other opium producing countries. This study presented new insights into the application of IR and ML to rapid drug profiling analysis.


Assuntos
Ópio , Espectroscopia de Luz Próxima ao Infravermelho , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Afeganistão , Mianmar , Espectrofotometria Infravermelho , Análise Discriminante , Análise dos Mínimos Quadrados , Máquina de Vetores de Suporte
4.
J Neuroinflammation ; 20(1): 261, 2023 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-37953259

RESUMO

BACKGROUND: Subarachnoid hemorrhage (SAH) causes significant long-term neurocognitive dysfunction, which is associated with hippocampal neuroinflammation. Growing evidences have shown that astrocytes played a significant role in mediating neuroinflammation. Recently, in vivo reprogramming of astrocytes to neurons by NeuroD1 or PTBP1 administration has generated a lot of interests and controversies. While the debates centered on the source of neurogenesis, no attention has been paid to the changes of the astrocytes-mediated neuroinflammation and its impact on endogenous neurogenesis after NeuroD1 administration. METHODS: 80 adult male C57BL/6 mice were used in this study. SAH was established by pre-chiasmatic injection of 100 µl blood. AAV-NeuroD1-GFP virus was injected to the hippocampus 3 day post-SAH. Neurocognitive function, brain water content, in vivo electrophysiology, Golgi staining, western blot and immunofluorescent staining were assessed at day 14 post-virus injection. RESULTS: NeuroD1 administration markedly attenuated reactive astrocytes-mediated neuroinflammation by reversing neurotoxic A1 astrocytes transformation, decreasing the secretion of neuroinflammatory cytokines, and reducing the activation of harmful microglia. NeuroD1 treatment significantly reversed the brain-blood barrier impairment and promoted the release of neurotrophic factors pleiotrophin (PTN), all of which contributed to the improvement of cellular microenvironment and made it more suitable for neurogenesis. Interestingly, besides neurogenesis in the hippocampus from cells transfected with NeuroD1 at the early phase of SAH, NeuroD1 administration significantly boosted the endogenous neurogenesis at the late phase of SAH, which likely benefited from the improvement of the neuroinflammatory microenvironment. Functionally, NeuroD1 treatment significantly alleviated neurocognitive dysfunction impaired by SAH. CONCLUSIONS: NeuroD1 significantly promoted neurofunctional recovery by attenuating reactive astrocytes-mediated neuroinflammation and boosting neurogenesis decimated by SAH. Specifically, NeuroD1 efficiently converted transfected cells, most likely astrocytes, to neurons at the early phase of SAH, suppressed astrocytes-mediated neuroinflammation and boosted endogenous neurogenesis at the late phase of SAH.


Assuntos
Doenças Neuroinflamatórias , Hemorragia Subaracnóidea , Camundongos , Animais , Masculino , Hemorragia Subaracnóidea/complicações , Hemorragia Subaracnóidea/tratamento farmacológico , Camundongos Endogâmicos C57BL , Encéfalo , Neurogênese/fisiologia
5.
Drug Test Anal ; 2023 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-37997567

RESUMO

This study presents a new strategy to discriminate between opium samples obtained from different geographical regions. Nuclear magnetic resonance (NMR) profiling and chemometrics were applied to geographical classification of opium originating from Myanmar and Afghanistan, which are two major opium producing countries in the world. A total of 50 Myanmar and 46 Afghanistan authentic opium samples were analyzed by 1 H-NMR, and the chemical profiles were characterized. Different sample preparation procedures, data processing methods, and chemometrics were compared to obtain the best classification effect. It was found that drying and the addition of buffer solutions were unnecessary for classification purposes; thus, the gum opium samples were extracted directly with CD3 OD, which shortened sample preparation time. A full discrimination between the two geographical origins was achieved by 1 H-NMR profiling and orthogonal partial least squares discriminant analysis. All 30 opium samples were classified correctly by the developed orthogonal partial least squares discriminant analysis model. Compared with traditional chromatography and mass spectrometry profiling methods, the 1 H-NMR profiling method was faster (with instrument analysis time of less than 3 min) and reproducible. This study provides new insights into the applying of NMR profiling and chemometrics to rapid drug profiling analysis.

6.
Nat Food ; 4(9): 740-741, 2023 09.
Artigo em Inglês | MEDLINE | ID: mdl-37653046

Assuntos
Amônia , Fazendeiros , Humanos
7.
Infect Drug Resist ; 16: 651-659, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36743337

RESUMO

Purpose: To explore the value of integrating clinical and computed tomography (CT) features to predict multidrug-resistant pulmonary tuberculosis (MDR-PTB). Patients and Methods: The study included 212 patients with MDR-PTB and 180 patients with drug-sensitive pulmonary tuberculosis (DS-PTB) who referred to our institute in China between January 2016 and March 2021. The clinical and CT characteristics were analyzed and compared between both groups. Multivariable logistic regression analysis was performed to identify independent factors that can be used to predict MDR-PTB. Furthermore, 115 patients admitted to another center from January 2019 to January 2022 were included as external validation cohort. Results: For clinical characteristics, five parameters were significantly different between the two groups (all P < 0.05). With regard to CT features, nine parameters were significantly different between the two groups (all P < 0.05). Multivariable logistic regression analysis using the aforementioned differential features showed that male sex, retreated history, longer duration of previous anti-TB treatment, lower CD4+ T lymphocyte count, thick-walled cavity, centrilobular micronodules and tree-in-bud sign, bronchial stenosis, pleural and pericardial thickening were the most effective variations associated with MDR-PTB with an area under the curve (AUC) of 0.849 and accuracy of 78.6%. Furthermore, the external validation cohort that contains 115 patients obtained an AUC of 0.933 and accuracy of 81.7%. Conclusion: MDR-PTB and DS-PTB have different clinical and imaging characteristics. A combined model incorporating these differential features can promptly diagnose MDR-PTB and develop subsequent therapeutic strategies.

8.
J Sep Sci ; 46(1): e2200656, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36285382

RESUMO

This paper reports a method for determining the carbonate content in barite ore using headspace gas chromatography. Based on the acidification reaction, the carbonate in the barite ore was converted to CO2 in a closed headspace vial. When the carbonate content was significant, the pressure caused changes in the CO2 and O2 signals and affected the measurement accuracy. It was found that carbonate content is proportional to the intensity ratio of the CO2 to O2 signals. Thus, the carbonate content in barite ore can be measured indirectly using a theoretical model. The results showed that the carbonate in 3 g of barite ore sample with a particle size of 74 µm could react completely with a hydrochloric acid solution (2 mol/L) at 65°C for 5 min. The method described herein had good precision (relative standard deviation < 4.14%) and accuracy (relative differences < 6.12%). Further, the limit of quantification was 0.07 mol/L. Owing to its simplicity and speed, this method can be used for the batch determination of carbonate content in barite ore.


Assuntos
Sulfato de Bário , Dióxido de Carbono , Carbonatos , Ácidos , Cromatografia Gasosa/métodos
9.
Sci Total Environ ; 854: 158736, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36122720

RESUMO

Atmospheric nitrate (NO3-) produced by photochemical oxidation in the atmosphere has high oxygen isotope ratios (δ18O values). Recently, the primary NO3- emitted from combustion sources was found to have much lower δ18O values. However, it is unclear how and to what extents the low δ18O signatures were controlled by major O sources during the primary NO3- formation of combustion processes. Here, we first measured concentrations and δ18O values of NO3- from burning five biomass materials (bb-NO3- and δ18Obb-NO3-, respectively) in China. Distinctly higher concentration levels of the bb-NO3- emissions (42.1 ± 8.1 µmol m-3) than ambient NO3- suggest it is a potential source of atmospheric NO3- pollution. Much lower δ18Obb-NO3- signatures (27.6 ± 2.7 ‰) than ambient NO3- support it as a primary emission source with different O sources and formation mechanism from secondary NO3-. Isotope mass-balance modeling revealed that atmospheric O2 and the biomass O dominated the O of bb-NO3- (53 ± 7 % and 40 ± 4 %, respectively) over the aqueous vapor (7 ± 3 %). Besides, we found increasing δ18Obb-NO3- values with the biomass N contents and relatively lower δ18Obb-NO3- values for biomasses with higher carbon (C) and lower O contents, indicating that biomass C, N, and O contents may influence the source O contributions of the bb-NO3-. This work provides a novel isotope analysis on the O source contribution of the bb-NO3-, which is useful for understanding the formation mechanism of combustion-related NO3- sources and evaluating the primary NO3- emissions.

10.
Nat Commun ; 13(1): 7710, 2022 12 13.
Artigo em Inglês | MEDLINE | ID: mdl-36513669

RESUMO

Atmospheric ammonia (NH3) and ammonium (NH4+) can substantially influence air quality, ecosystems, and climate. NH3 volatilization from fertilizers and wastes (v-NH3) has long been assumed to be the primary NH3 source, but the contribution of combustion-related NH3 (c-NH3, mainly fossil fuels and biomass burning) remains unconstrained. Here, we collated nitrogen isotopes of atmospheric NH3 and NH4+ and established a robust method to differentiate v-NH3 and c-NH3. We found that the relative contribution of the c-NH3 in the total NH3 emissions reached up to 40 ± 21% (6.6 ± 3.4 Tg N yr-1), 49 ± 16% (2.8 ± 0.9 Tg N yr-1), and 44 ± 19% (2.8 ± 1.3 Tg N yr-1) in East Asia, North America, and Europe, respectively, though its fractions and amounts in these regions generally decreased over the past decades. Given its importance, c-NH3 emission should be considered in making emission inventories, dispersion modeling, mitigation strategies, budgeting deposition fluxes, and evaluating the ecological effects of atmospheric NH3 loading.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Amônia/análise , Poluentes Atmosféricos/análise , Ecossistema , Monitoramento Ambiental/métodos , Nitrogênio/análise , China
11.
Front Med (Lausanne) ; 9: 930678, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36250101

RESUMO

Background: Talaromyces marneffei (T. marneffei) is an opportunistic fungal pathogen commonly found in human immunodeficiency virus (HIV) patients that often infects lymph nodes. Knowledge about the computed tomography (CT) characteristics of T. marneffei lymphadenopathy in HIV patients is limited. The aim of this study was to investigate the clinical and CT characteristics of T. marneffei lymphadenopathy to improve its diagnosis and promote recognition of this type of infection in radiology. Methods: Between February 2019 and June 2021, we retrospectively reviewed the clinical features and CT characteristics of T. marneffei lymphadenopathy in 21 HIV patients. Results: The clinical symptoms of T. marneffei infection are non-specific. Anemia (100%), fever (85.7%) and cough and sputum production (76.2%) were the most frequent symptoms. Multiple lymphadenopathies, mainly in the mediastinum (76.2%) and mesentery (82.4%), can be fused (14.3%) and necrotic (52.4%), with slight (41.7%) and moderate enhancement (58.3%) that is heterogeneous. In addition to involving the lymph nodes, the lesions involved the lungs (81.0%), liver and spleen (42.9%), and small intestine (14.3%). Conclusions: T. marneffei is prone to affecting lymph nodes and extranodal organs in HIV patients. Although the clinical manifestations of T. marneffei infection are not specific, the possibility of T. marneffei infection should be considered if CT findings indicate multiple lesion sites.

12.
J Chromatogr A ; 1683: 463547, 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36228572

RESUMO

Barium sulfate (BaSO4) content is used to evaluate the grade of barite ore. In the present study, we report a method to determine the BaSO4 content in barite ore by phase conversion-headspace gas chromatography with partial pressure correction. In this method, the ore sample is roasted with sodium carbonate and potassium carbonate after pretreatment with hydrochloric acid. The roasted product is subsequently placed in a closed headspace bottle to react with hydrochloric acid. The ratio of CO2 to O2 signals is detected by a thermal conductivity detector for gas chromatography. Finally, the BaSO4 content in barite ore is calculated using this ratio. The method demonstrates good precision (relative standard deviation < 0.84%) and accuracy (relative error < 3.40%), with the uncertainty at 95% confidence interval at approximately +/- 0.57%. Moreover, this approach is expected to be used for the batch testing of BaSO4 content in barite ores in industrial applications.


Assuntos
Sulfato de Bário , Dióxido de Carbono , Sulfato de Bário/química , Pressão Parcial , Ácido Clorídrico , Cromatografia Gasosa/métodos
13.
Front Pharmacol ; 13: 1026836, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36278197

RESUMO

Objective: To investigate the therapeutic effect and influencing factors of perampanel (PER) on electrical status epilepticus during sleep (ESES). Methods: We retrospectively analyzed the clinical data of pediatric patients with focal epilepsy and ESES who were treated at the Epilepsy Center of Shengjing Hospital of China Medical University between January 2016 and March 2022. Changes in the spike wave index (SWI) after 24 weeks of PER add-on treatment were compared. Kaplan‒Meier survival analysis, the log-rank test and multivariate Cox regression analysis were performed. Results: A total of 54 pediatric patients met the inclusion criteria, including 33 males and 21 females. The mean age at the diagnosis of epilepsy was 6.41 ± 2.14 years and at ESES diagnosis was 7.58 ± 2.40 years. The mean ESES duration before add-on PER was 25.31 ± 15.12 months. The mean age of the patients at add-on PER initiation was 9.69 ± 2.12 years. The ESES resolved in 29 children after 6 months of PER add-on treatment, and the response rate was 53.7%. Univariate analysis with the log-rank test showed that the therapeutic effect of PER differed according to the age at ESES diagnosis and ESES duration before add-on PER treatment. Multivariate Cox regression analysis showed that only ESES duration before PER administration was a risk factor for PER treatment failure, and the other factors had no effect on the therapeutic effect. Conclusion: PER add-on treatment has a good therapeutic effect on ESES and can be used as an alternative to corticosteroid and benzodiazepines. The therapeutic effect of PER add-on treatment was not related to the dose. A longer ESES duration results in a worse therapeutic effect. Therefore, more aggressive treatment measures should be implemented for ESES.

14.
Natl Sci Rev ; 9(9): nwac106, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36128454

RESUMO

Nitrogen oxides (NOx, the sum of nitric oxide (NO) and N dioxide (NO2)) emissions and deposition have increased markedly over the past several decades, resulting in many adverse outcomes in both terrestrial and oceanic environments. However, because the microbial NOx emissions have been substantially underestimated on the land and unconstrained in the ocean, the global microbial NOx emissions and their importance relative to the known fossil-fuel NOx emissions remain unclear. Here we complied data on stable N isotopes of nitrate in atmospheric particulates over the land and ocean to ground-truth estimates of NOx emissions worldwide. By considering the N isotope effect of NOx transformations to particulate nitrate combined with dominant NOx emissions in the land (coal combustion, oil combustion, biomass burning and microbial N cycle) and ocean (oil combustion, microbial N cycle), we demonstrated that microbial NOx emissions account for 24 ± 4%, 58 ± 3% and 31 ± 12% in the land, ocean and global environment, respectively. Corresponding amounts of microbial NOx emissions in the land (13.6 ± 4.7 Tg N yr-1), ocean (8.8 ± 1.5 Tg N yr-1) and globe (22.5 ± 4.7 Tg N yr-1) are about 0.5, 1.4 and 0.6 times on average those of fossil-fuel NOx emissions in these sectors. Our findings provide empirical constraints on model predictions, revealing significant contributions of the microbial N cycle to regional NOx emissions into the atmospheric system, which is critical information for mitigating strategies, budgeting N deposition and evaluating the effects of atmospheric NOx loading on the world.

15.
Environ Pollut ; 311: 119969, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-35981639

RESUMO

The elevation of nitrogen (N) deposition by urbanization profoundly impacts the structure and function of surrounding forest ecosystems. Plants are major biomass sinks of external N inputs into forests. Yet, the N-use strategies of forest plants in many areas remain unconstrained in city areas, so their responses and adapting mechanisms to the elevated N deposition are open questions. Here we investigated concentrations and N isotope (δ15N) of total N (TN) and nitrate (NO3-) in leaves and roots of four plant species in subtropical shrubberies and pine forests under N deposition levels of 13 kg-N ha-1 yr-1 and 29 kg-N ha-1 yr-1 at the Guiyang area of southwestern China, respectively. The δ15N differences between plant NO3- and soil NO3- revealed a meager NO3- reduction in leaves but a preferentially high NO3- reduction in roots. δ15N mass-balance analyses between plant TN and soil dissolved N suggested that soil NO3- contributed more than reduced N, and dissolved organic N contributed comparably with ammonium to plant TN, and the study plants preferred NO3- over reduced N. The elevation of N deposition induced root but not leaf NO3- reduction and enhanced the contribution of soil NO3- to plant TN, but plant NO3- preference decreased due to much higher magnitudes of soil NO3- enrichment than plant NO3- utilization. We conclude that plants in subtropical forests of southwestern China preferred NO3- over reduced N, and NO3- was reduced more in roots than in leaves, anthropogenic N pollution enhanced soil NO3- enrichment and plant NO3- utilization but reduced plant NO3- preference.


Assuntos
Ecossistema , Nitrogênio , China , Florestas , Nitrogênio/análise , Plantas , Solo/química
16.
Org Biomol Chem ; 20(35): 7099-7104, 2022 09 14.
Artigo em Inglês | MEDLINE | ID: mdl-36040323

RESUMO

An efficient method to construct unique spiro[indoline-3,4'-pyrrolo[3,4-b]pyridines] was successfully developed via a DABCO promoted formal [3 + 3] cycloaddition reaction of MBH carbonates of isatins with ß-enamino maleimides in acetonitrile at room temperature. This reaction afforded multifunctionalized spiro[indoline-3,4'-pyrrolo[3,4-b]pyridines] and spiro[dipyrrolo[3,4-b:3',4'-e]pyridine-8,3'-indolines] in good yields and with lower diastereoselectivity. The relative configuration of the two diasteromers of the spiro compounds was clearly elucidated by the determination of eight single crystal structures.


Assuntos
Isatina , Compostos de Espiro , Carbonatos , Reação de Cicloadição , Indóis , Isatina/química , Maleimidas/química , Estrutura Molecular , Piridinas , Compostos de Espiro/química
17.
J Innate Immun ; 14(4): 366-379, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35780770

RESUMO

As a serious and elusive syndrome caused by infection, sepsis causes a high rate of mortality around the world. Our investigation aims at exploring the role and possible mechanism of specificity protein-1 (SP1) in the development of sepsis. A mouse model of sepsis was established by cecal ligation perforation, and a cellular model was stimulated by lipopolysaccharide (LPS), followed by determination of the SP1 expression. It was determined that SP1 was poorly expressed in the intestinal tissues of septic mice and LPS-treated cells. Next, we examined the interactions among SP1, histone deacetylase 4 (HDAC4), and high mobility group box 1 (HMGB1) and found that SP1 bound to the HDAC4 promoter to upregulate its expression, thereby promoting the deacetylation of HMGB1. Meanwhile, gain- or loss-of-function approaches were applied to evaluate the intestinal barrier dysfunction, oxidative stress, and inflammatory response. Overexpression of SP1 or underexpression of HMGB1 was observed to reduce intestinal barrier dysfunction, oxidative stress, and inflammatory injury. Collectively, these experimental data provide evidence reporting that SP1 could promote the HDAC4-mediated HMGB1 deacetylation to reduce intestinal barrier dysfunction, oxidative stress, and inflammatory response induced by sepsis, providing a novel therapeutic target for sepsis prevention and treatment.


Assuntos
Gastroenteropatias , Proteína HMGB1/genética , Histona Desacetilases/genética , Sepse , Fator de Transcrição Sp1/metabolismo , Animais , Proteína HMGB1/metabolismo , Histona Desacetilases/metabolismo , Histona Desacetilases/uso terapêutico , Células Endoteliais da Veia Umbilical Humana/metabolismo , Humanos , Lipopolissacarídeos/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Estresse Oxidativo , Sepse/tratamento farmacológico
18.
Sci Total Environ ; 838(Pt 3): 156405, 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-35660601

RESUMO

To examine the perturbation of atmospheric nitrogen (N) deposition on soil N status and the biogeochemical cycle is meaningful for understanding forest function evolution with environmental changes. However, levels of soil bioavailable N and their environmental controls in forests receiving high atmospheric N deposition remain less investigated, which hinders evaluating the effects of enhanced anthropogenic N loading on forest N availability and N losses. This study analyzed concentrations of soil extractable N, microbial biomass N, net rates of N mineralization and nitrification, and their relationships with environmental factors among 26 temperate forests under the N deposition rates between 28.7 and 69.0 kg N ha-1 yr-1 in the Beijing-Tianjin-Hebei (BTH) region of northern China. Compared with other forests globally, forests in the BTH region showed higher levels of soil bioavailable N (NH4+, 27.1 ± 0.8 mg N kg-1; NO3-, 7.0 ± 0.8 mg N kg-1) but lower net rates of N mineralization and nitrification (0.5 ± 0.1 mg N kg-1 d-1 and 0.4 ± 0.1 mg N kg-1 d-1, respectively). Increasing N deposition levels increased soil nitrification and NO3- concentrations but did not increase microbial biomass N and N mineralization among the study forests. Soil moisture and C availability were found as dominant factors influencing microbial N mineralization and bioavailable N. In addition, by budgeting the differences in soil total N densities between the 2000s and 2010s, atmospheric N inputs to the forests were more retained in soils than lost proportionally (84% vs. 16%). We concluded that the high N deposition enriched soil N without stimulating microbial N mineralization among the study forests. These results clarified soil N status and the major controlling factors under high anthropogenic N loading, which is helpful for evaluating the fates and ecological effects of atmospheric N pollution.


Assuntos
Nitrogênio , Solo , China , Florestas , Nitrificação , Nitrogênio/análise , Microbiologia do Solo
19.
Mol Med ; 28(1): 9, 2022 01 28.
Artigo em Inglês | MEDLINE | ID: mdl-35090386

RESUMO

BACKGROUND: Sepsis is a troublesome syndrome that can cause intestinal injury and even high mortality rates. Omega-3 fatty acids (FAs) are known to protect against intestinal damage. Accordingly, the current study set out to explore if omega-3 FAs could affect sepsis-induced intestinal injury with the involvement of the microRNA (miR)-1-3p/Notch3-Smad axis. METHODS: First, cecal ligation and perforation (CLP) was performed to establish septic mouse models in C57BL/6J mice, and mouse intestinal epithelial MODE-K cells were induced by lipopolysaccharide (LPS) to establish sepsis cell models. The CLP-induced septic mice or LPS-exposed cells were subjected to treatment with Omega-3 FAs and activin (Smad signaling activator), miR-1-3p inhibitor and over-expressed/short hairpin RNA (oe-/sh)-Notch3 to explore their roles in inflammation, intestinal oxidative stress and cell apoptosis. A dual-luciferase reporter gene assay was further performed to verify the regulatory relationship between miR-1-3p and Notch3. RESULTS: Omega-3 FAs inhibited CLP-induced intestinal injury and ameliorated LPS-induced intestinal epithelial cell injury by down-regulating miR-1-3p, as evidenced by decreased levels of tumor necrosis factor-α, interleukin-1ß (IL-1ß) and IL-6, in addition to diminished levels of reactive oxygen species, malondialdehyde levels and superoxide dismutase activity. Furthermore, miR-1-3p could down-regulate Notch3, which inactivated the Smad pathway. CONCLUSION: Collectively, our findings indicated that omega-3 FAs elevate the expression of Notch3 by down-regulating miR-1-3p, and then blocking the Smad pathway to alleviate intestinal epithelial inflammation and oxidative stress injury caused by sepsis.


Assuntos
Ácidos Graxos Ômega-3/metabolismo , Regulação da Expressão Gênica , Enteropatias/etiologia , Enteropatias/metabolismo , MicroRNAs/genética , Receptor Notch3/genética , Sepse/complicações , Animais , Biomarcadores , Gerenciamento Clínico , Modelos Animais de Doenças , Suscetibilidade a Doenças , Perfilação da Expressão Gênica , Enteropatias/diagnóstico , Enteropatias/terapia , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Camundongos , Modelos Biológicos , Estresse Oxidativo , Receptor Notch3/metabolismo , Sepse/etiologia , Transdução de Sinais , Proteínas Smad
20.
Sci Total Environ ; 806(Pt 3): 151203, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34710420

RESUMO

Exotic plant invasion is an urgent issue occurring in the biosphere, which can be stimulated by environmental nitrogen (N) loading. However, the allocation and assimilation of soil N sources between leaves and roots remain unclear for plants in invaded ecosystems, which hampers the understanding of mechanisms behind the expansion of invasive plants and the co-existence of native plants. This work established a new framework to use N concentrations and isotopes of soils, roots, and leaves to quantitatively decipher intra-plant N allocation and assimilation among plant species under no invasion and under the invasion of Chromolaena odorata and Ageratina adenophora in a tropical ecosystem of SW China. We found that the assimilation of N derived from both soil ammonium (NH4+) and nitrate (NO3-) were higher in leaves than in roots for invasive plants, leading to higher leaf N levels than native plants. Compared with the same species under no invasion, most native plants under invasion showed higher N concentrations and NH4+ assimilations in both leaves and roots, and increases in leaf N were higher than in root N for native plants under invasion. These results inform that preferential N allocation, dominated by NH4+-derived N, to leaves over roots as an important N-use strategy for plant invasion and co-existence in the studied tropical ecosystem.


Assuntos
Ecossistema , Nitrogênio , Isótopos , Nitrogênio/análise , Folhas de Planta/química , Raízes de Plantas/química , Solo
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