High-resolution medical image reconstruction based on residual neural network for diagnosis of cerebral aneurysm.

Objective: Cerebral aneurysms are classified as severe cerebrovascular diseases due to hidden and critical onset, which seriously threaten life and health. An effective strategy to control intracranial aneurysms is the regular diagnosis and timely treatment by CT angiography (CTA) imaging technology. However, unpredictable patient movements make it challenging to capture sub-millimeter-level ultra-high resolution images in a CTA scan. In order to improve the doctor's judgment, it is necessary to improve the clarity of the cerebral aneurysm medical image algorithm. Methods: This paper mainly focuses on researching a three-dimensional medical image super-resolution algorithm applied to cerebral aneurysms. Although some scholars have proposed super-resolution reconstruction methods, there are problems such as poor effect and too much reconstruction time. Therefore, this paper designs a lightweight super-resolution network based on a residual neural network. The residual block structure removes the B.N. layer, which can effectively solve the gradient problem. Considering the high-resolution reconstruction needs to take the complete image as the research object and the fidelity of information, this paper selects the channel domain attention mechanism to improve the performance of the residual neural network. Results: The new data set of cerebral aneurysms in this paper was obtained by CTA imaging technology of patients in the Department of neurosurgery, the second affiliated of Guizhou Medical University Hospital. The proposed model was evaluated from objective evaluation, model effect, model performance, and detection comparison. On the brain aneurysm data set, we tested the PSNR and SSIM values of 2 and 4 magnification factors, and the scores of our method were 33.01, 28.39, 33.06, and 28.41, respectively, which were better than those of the traditional SRCNN, ESPCN and FSRCNN. Subsequently, the model is applied to practice in this paper, and the effect, performance index and diagnosis of auxiliary doctors are obtained. The experimental results show that the high-resolution image reconstruction model based on the residual neural network designed in this paper plays a more influential role than other image classification methods. This method has higher robustness, accuracy and intuition. Conclusion: With the wide application of CTA images in the clinical diagnosis of cerebral aneurysms and the increasing number of application samples, this method is expected to become an additional diagnostic tool that can effectively improve the diagnostic accuracy of cerebral aneurysms.

Combining potassium chloride leaching with vertical electrokinetics to remediate cadmium-contaminated soils.

This study evaluated the feasibility of combining potassium chloride (KCl) leaching and electrokinetic (EK) treatment for the remediation of cadmium (Cd) and other metals from contaminated soils. KCl leaching was compared at three concentrations (0.2%, 0.5%, and 1% KCl). EK treatment was conducted separately to migrate the metals in the topsoil to the subsoil. The combined approach using KCl leaching before or after EK treatment was compared. For the single vertical EK treatment, the removal of Cd, lead (Pb), copper (Cu) and zinc (Zn) from the topsoil (0-20 cm) was 9.38%, 4.80%, 0.95%, and 10.81%, respectively. KCl leaching at 1% KCl removed 84.06% Cd, 9.95% Pb, 4.34% Cu, and 19.93% Zn from the topsoil, with higher removal efficiency than that of the 0.2% and 0.5% KCl leaching treatments. By combining the KCl leaching and EK treatment, the removal efficiency of heavy metals improved, in particular for the 1% KCl + EK treatment, where the removal rate of Cd, Pb, Cu, and Zn from the upper surface soil reached 97.79%, 17.69%, 14.37%, and 41.96%, respectively. Correspondingly, the soil Cd content decreased from 4 to 0.21 mg/kg, and was below the Chinese standard limit of 0.3 mg/kg soil. These results indicate that 1% KCl + EK treatment is a good combination technique to mitigate Cd pollution from contaminated soils used for growing rice and leafy vegetables.

Diurnal Expression of the Per2 Gene and Protein in the Lateral Habenular Nucleus.

The suprachiasmatic nucleus plays an important role in generating circadian rhythms in mammals. The lateral habenular nucleus (LHb) is closely linked to this structure. Interestingly, the LHb shows a rhythmic firing rate in vivo and in vitro, and sustained oscillation of rhythmic genes in vitro. However, under the in vivo condition, whether rhythmic gene expression in the LHb has circadian rhythms remains unknown. In this study, we examined LHb tissue in rats to determine Period2 (Per2) gene and protein expression at six zeitgeber time points (ZT2, ZT6, ZT10, ZT14, ZT18, and ZT22) in a 12-h light and 12-h dark (LD) environment. We found that in the LD environment, Per2 gene expression and PER2 protein levels in the LHb were higher in the day and lower in the night, showing periodic oscillation, with a peak at ZT10 and a trough at ZT22 (Per2 mRNA) and ZT18 (PER2 protein). We conclude that Per2 expression and PER2 protein levels in the LHb have rhythmic oscillation in vivo. This study provides a basis for further study on the role of the LHb in the circadian rhythm system.

Zhichan powder regulates nigrostriatal dopamine synthesis and metabolism in Parkinson's disease rats.

In this study, rat models of Parkinson's disease induced by substantia nigra injection of 6-hydroxy-dopamine were intragastrically administered Zhichan powder daily for 50 days. Reverse transcription PCR results showed that tyrosine hydroxylase mRNA expression in the rat substantia nigra was significantly increased, while monoamine oxidase B mRNA expression was significantly decreased in the Zhichan powder group, compared with the model group. In addition, the levels of striatal dopamine and homovanillic acid, the ratio of dopamine to homovanillic acid, and the activity of blood superoxide dismutase were all higher in the Zhichan powder group than in the model group, but the content of malondialdehyde in blood was lower. Our experimental findings indicate that Zhichan powder has an antioxidant effect, it can regulate the expression of monoamine oxidase B and tyrosine hydroxylase in the substantia nigra of Parkinson's disease rats, and it can facilitate the secretion of striatal dopamine and its metabolite homovanillic acid.

Effects of Zhichan powder on signal transduction and apoptosis-associated gene expression in the substantia nigra of Parkinson's disease rats.

Previous studies have shown that Zhichan powder elevated immunity and suppressed oxidation in mice. Rat models of Parkinson's disease were induced by stereotaxically injecting 6-hydroxydopamine into the substantia nigra. The rat models were intragastrically treated with Zhichan powder, which is composed of milkvetch root, ginseng, bunge swallowwort root, himalayan teasel root, Magnolia officinalis, Ligustrum lucidum Ait. and szechwan lovage rhizome. Immunohistochemistry and reverse transcription-PCR results demonstrated that mRNA and protein expression of tumor necrosis factor receptor 1, Fas, caspase-8, cytochrome C, Bax, caspase-3, and p53 significantly increased, but Bcl-2 expression significantly decreased in the substantia nigra of rats with Parkinson's disease. Following Zhichan powder administration, mRNA and protein expression of tumor necrosis factor receptor 1, Fas, caspase-8, cytochrome C, Bax, caspase-3, and p53 diminished, but Bcl-2 expression increased in the rat substantia nigra. These results indicate that Zhichan powder regulates signal transduction protein expression, inhibits apoptosis, and exerts therapeutic effects on Parkinson's disease.