Improvement of Fe(VI) oxidation by NaClO on degrading phenolic substances and reducing DBPs formation potential.

Ferrate(VI) is a green oxidant and can effectively oxidize micropollutants. However, the instability of Fe(VI), i.e., self-decomposition, in the aqueous solution limited its application. Herein, it was found that the degradation of phenolic substances had been substantially improved through the combination of Fe(VI) with NaClO. At the condition of pH 8.0, 50 µM of Fe(VI) degraded 18.66 % of BPA (bisphenol A) at 0.5 min or 21.67 % of phenol at 2 min. By contrast, Fe(VI)/NaClO (50/10 µM) oxidized 38.21 % of BPA at 0.5 min or 38.08 % of phenol at 2 min with a synergistic effect. At the end of the reaction, the concentration of Fe(VI) in Fe(VI)/NaClO (50/10 µM) was 28.97 µM for BPA degradation, higher than the 25.62 µM of Fe(VI) group. By active species analysis, intermediate iron species [i.e., Fe(V) and Fe(IV)] played a vital role in the synergistic effect in Fe(VI)/NaClO system, which would react with the applied NaClO to regenerate Fe(VI). In natural water, the Fe(VI)/NaClO could also degrade phenolic substances of natural organic matter (NOM). Although the NaClO reagent was applied, disinfection by-products (DBPs) formation potential decreased by 22.75 % of the raw sample after Fe(VI)/NaClO treatment. Significantly, THMs, mainly caused by phenolic substances of NOM, even declined by 29.18 % of raw sample. Based on that, this study explored a novel ferrate(VI) oxidation system using the cheap NaClO reagent, which would present a new insight on ferrate(VI) application.

Role of nano-Fe3O4 particle on improving membrane bioreactor (MBR) performance: Alleviating membrane fouling and microbial mechanism.

This study would investigate the effect of nano-Fe3O4 particles on the performance of membrane bioreactor (MBR), including membrane fouling, membrane rejection and microbial community. It can effectively alleviate membrane fouling and improve the effluent quality in MBR by bio-effect rather than nanoparticle adsorption. The lowest membrane fouling resistance was achieved at R4-MBR (sludge and membrane surface with nano-Fe3O4), which decreased by 46.08%. Meanwhile, R3-MBR (sludge with nano-Fe3O4) had the lowest concentration of COD in effluent which was below 20 mg/L in the stable phase of MBR operation. After applying nano-Fe3O4, the content of extracellular polymeric substances (EPS) and soluble microbial products (SMP) were both reduced with a lower molecular weight. From the microbial community analysis, the abundance of Proteobacteria increased from 25.06 to 45.11% at the phylum level in R3-MBR. It contributed to removing organic substances in MBRs. Moreover, the nano-Fe3O4 restricted Bacteroidetes growth, especially in R4-MBR, leading to a more excellent performance of membrane flux. Besides, the applied nano-Fe3O4 promoted the abundance of Quorum Quenching (QQ) microorganism, and declined the percentage of Quorum Sensing (QS) bacteria. Then, a lower content of N-Acyl-l-Homoserine Lactones (AHLs) in containing nano-Fe3O4 sludge. That was also prone to control membrane fouling. Overall, this study indicates the nano-Fe3O4 particle is appropriate for elevating MBR performance, such as membrane fouling and effluent quality, by bio-effect.

Waste sludge disintegration, methanogenesis and final disposal via various pretreatments: Comparison of performance and effectiveness.

This study compared the three wastewater pretreatments of ozonation, Fe2+-S2O8 2- and freeze-thawing (F/T) in the disintegration, anaerobic digestion (AD) and final disposal of the sludge. The F/T pretreatment increased the dewaterability and settleability of the sludge by 7.8% and 47.1%, respectively. The ozonation pretreatment formed more volatile fatty acids (VFAs), with a peak value of 320.82 mg SCOD/L and controlled the release of sulfides. The Fe2+-S2O8 2- pretreatment removed heavy metals through the absorption and flocculation of ferric particles formed in-situ. During the anaerobic digestion of the sludge, the ozonation pretreatment accelerated the hydrolysis rate (k) rather than the biochemical methane potential (B0) of the sludge due to the high VFA content in the supernatant. Comparatively, the F/T pretreatment facilitated the B0 with great economic efficiency by enhancing the solubilisation of the sludge. Although Fe2+-S2O8 2- pretreatment decreased the methane production, the ferric particle was a unique advantage in the disintegration and harmless disposal of the sludge. The digested sludge had more VFAs after ozonation pretreatment, which contributed to the recycling of carbon. In addition, the lower sludge volume could save the expense of transportation and disposal by ozonation pretreatment. Different pretreatments had different characteristics. The comparative study provided information allowing the selection of the type of pretreatment to achieve different objectives of the treatment and disposal of sludge.

The PLEXIN PLX-2 and the ephrin EFN-4 have distinct roles in MAB-20/Semaphorin 2A signaling in Caenorhabditis elegans morphogenesis.

Semaphorins are extracellular proteins that regulate axon guidance and morphogenesis by interacting with a variety of cell surface receptors. Most semaphorins interact with plexin-containing receptor complexes, although some interact with non-plexin receptors. Class 2 semaphorins are secreted molecules that control axon guidance and epidermal morphogenesis in Drosophila and Caenorhabditis elegans. We show that the C. elegans class 2 semaphorin MAB-20 binds the plexin PLX-2. plx-2 mutations enhance the phenotypes of hypomorphic mab-20 alleles but not those of mab-20 null alleles, indicating that plx-2 and mab-20 act in a common pathway. Both mab-20 and plx-2 mutations affect epidermal morphogenesis during embryonic and in postembryonic development. In both contexts, plx-2 null mutant phenotypes are much less severe than mab-20 null phenotypes, indicating that PLX-2 is not essential for MAB-20 signaling. Mutations in the ephrin efn-4 do not synergize with mab-20, indicating that EFN-4 may act in MAB-20 signaling. EFN-4 and PLX-2 are coexpressed in the late embryonic epidermis where they play redundant roles in MAB-20-dependent cell sorting.

Improved method for visualizing cells revealed dynamic morphological changes of ventral neuroblasts during ventral cleft closure of Caenorhabditis elegans.

The formation of intricate and functional biological structures depends on the dynamic changes of cellular morphology. Confocal laser scanning microscopy (CLSM) is a widely used method to reveal the three-dimensional (3-D) structure of cells during the development of Caenorhabditis elegans (C. elegans) and other model organisms. Improving the efficiency and image quality of CLSM would benefit studies using this method. We found that CED-10::GFP::CED-10, a green fluorescent protein (GFP) marker, is intensely expressed beneath the cell surface, facilitating visualization of cellular morphology in C. elegans embryos. By combining the unique properties of this marker, and with the help of direct 3-D rendering of images obtained by CLSM, we developed a simple but powerful method for investigating cellular morphology in developing embryos. Using this method we, for the first time, document the dynamic changes in the morphology of ventral neuroblasts in vivo during ventral cleft closure.

C. elegans PlexinA PLX-1 mediates a cell contact-dependent stop signal in vulval precursor cells.

PLX-1 is a PlexinA transmembrane protein in Caenorhabditis elegans, and the transmembrane-type semaphorin, SMP-1, is a ligand for PLX-1. The SMP-1/PLX-1 system has been shown to be necessary for proper epidermal morphogenesis in the male tail and seam cells. Here, we show that the SMP-1/PLX-1 system also regulates vulval morphogenesis. In plx-1 and smp-1 mutants, hermaphrodites sometimes exhibit a protruding vulva or multiple vulva-like protrusions. Throughout the vulval development of plx-1 and smp-1 mutants, the arrangement of vulval cells is often disrupted. In the initial step of vulval morphogenesis, vulval precursor cells (VPCs) are generated normally but are subsequently arranged abnormally in mutants. Continuous observation revealed that plx-1 VPC fails to terminate longitudinal extension after making contact with neighbor VPCs. The arrangement defects of VPCs in plx-1 and smp-1 mutants are rescued by expressing the respective cDNA in VPCs. plx-1::egfp and smp-1::egfp transgenes are both expressed in all vulval cells, including VPCs, throughout vulval development. We propose that the SMP-1/PLX-1 system is responsible for a cell contact-mediated stop signal for VPC extension. Analyses using cell fate-specific markers showed that the arrangement defects of VPCs also affect cell fate specification and cell lineages, but in a relatively small fraction of plx-1 mutants.