[Content of some pro- and anti-inflammatory cytokines in blood sera of leptospirosis patients].

AIM: Study the content of some pro- and anti-inflammatory cytokines in blood sera of leptospirosis patients in dynamics of infectious process and the role of these cytokines in the disease immunopathogenesis. MATERIALS AND METHODS: The content of cytokines in blood sera was determined by a method based on xMAP technology with a standard panel consisting of 9 analytes: TNF-α, MCP-1, IL-8, IL-4, IL-6, IL-10, IL-1Ra, IL-12 (p70), IFN-γ. RESULTS: A significantly increased level of IL-8, IL-10, TNF-α was confirmed and the increased content of MCP-1 in leptospirosis patients compared with practically healthy donors was established for the first time. Correlations between cytokines during leptospirosis were detected. CONCLUSION: The data obtained show that cytokines play an important role in leptospirosis immunopathogenesis.

[The effect of low-dose irradiation and of age on the in vitro radiosensitivity of human lymphocytes].

The effect of low-dose irradiation and of age on the radiosensitivity of human lymphocytes was studies in two groups: control (67 people) and exposed to uncontrolled low-dose irradiation in past (165 people). Radiosensitivity of lymphocytes was estimated by the level of chromosome aberrations induced in vitro by gamma-radiation Cs137 at the dose 1.5 Gy. In exposed children the frequency of induced chromosome aberrations was higher and in the exposed adults--lower in comparison to the coresponding controls. To investigate an age response of the number of chromosome aberrations three statistical approaches were used: the correlation analysis of individual data, the correlation analysis of means for 10-years intervals, the comparison of 3 age groups. In control group no significant alteration in the level of induced chromosome aberrations with age was found. However the significant negative correlation between these two parameters was revealed in exposed group, which likely is due to the opposite direction of differences in radiosensitivity of exposed children and adults from the corresponding controls.

[The effect of age and low-dose irradiation on the chromosomal aberration frequency in human lymphocytes].

The effect of age and of low-dose irradiation on the base level of chromosomal aberrations in lymphocytes was studied in two human groups: control one (128 people) and exposed in past to uncontrolled low-dose irradiation (283 people). In exposed group the frequencies of all types of chromosome aberrations were higher comparing to control group. For the investigation of the age response of the number of chromosomal aberrations three statistical approaches were used: correlation analysis of individual data, correlation analysis of mean frequencies of chromosomal aberrations for 10 years intervals, comparison of 3 age groups (young, middle age and old). In control group the significant increase of the level of chromosomal aberrations with age was found only when six 10-year intervals were analysed. In exposed group significant age increase of chromosomal aberration frequency (particularly due to double fragments) was observed with all 3 approaches. Low-dose irradiation of people is supposed to cause the functional defects of repair systems, controlling the level of genetic damages and they accumulate more intensive through age.