RESUMO
OBJECTIVE: To probe a molecular marker method of accrediting fingerprinting of 27 kinds of common Umbelliferae Chinese herb medicinal plants by sequencing rDNA. METHOD: The rDNA sequences of the 27 breeds of common Umbelliferae Chinese herb medicinal plants were amplified, and were digested by restriction endonuclease, and were seperated via polypropylene electrophoresis, at last 6 breeds of them were sequenced. RESULTS: The rDNA sequence fragment we gained concluded ITS1, ITS2, 5.8S complete sequence and 18S, 26S part sequence. On the electrophoresis map of PCR products digested by restriction endonuclease MSP I, 27 breeds appeared 16 kinds of characteristic map, 11 of them differ from others; and PCR products digested by restriction endonuclease HaeIII, there appeared 5 kinds of characteristic map among 27 breeds, 3 of them differ from others. The sequenced result of 6 breeds showed genes whose length extented from 652bp to 656bp were acquired. These sequences of 3 breeds which showed the same electrophoresis map after digested by restriction endonuclease HaeIII exhibited great similarity according to similar phylogenetic tree constructed on rDNA sequence. CONCLUSION: The rDNA sequence character is effective molecular marker for classifying the different Umbellerae Chinese herb medicinal plants. And the method of sequencing rDNA surpassed that of restriction fragment long polymorphism (RFLP).
