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Chinese Journal of Clinical Pharmacology and Therapeutics
; (12): 24-29, 2021.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-1015095
RESUMO
AIM: The DNA plasmid lipidosome (LP) vaccine based VEGFR2 extracellular region (exVEGFR2) was prepared in order to provide a new approach for cancer active immunotherapy. METHODS: High fidelity PCR was used to amplify the target sequence of exVEGFR2 with two restriction site of Kpn and Xba. The plasmid of pCMV/exVEGFR2 was constructed by connected exVEGFR2 with pCMV empty plasmid. The activity of immune activation was detected by ELISA. CTLs mediated cytotoxic activity was analyzed by