1.
Eur J Biochem
; 268(14): 3964-73, 2001 Jul.
Artigo
em Inglês
| MEDLINE
| ID: mdl-11453990
RESUMO
In a series of four racemic phenoxyalkyl-alkyl carbinols, 1-phenoxy-2-hydroxybutane (1) is enantioselectively acetylated by Burkholderia cepacia (formerly Pseudomonas cepacia) lipase with an E value > or = 200, whereas for the other three racemates E was found to be < or = 4. To explain the high preference of B. cepacia lipase for (R)-(+)-1, a precursor of its transition state analogue with a tetrahedral P-atom, (R(P),S(P))-O-(2R)-(1-phenoxybut-2-yl)methylphosphonic acid chloride was prepared and crystallized in complex with B. cepacia lipase. The X-ray structure of the complex was determined, allowing to compare the conformation of the inhibitor with results of molecular modelling.
Assuntos
Burkholderia cepacia/enzimologia , Lipase/química , Compostos Organofosforados/metabolismo , Catálise , Cristalografia por Raios X , Ligação de Hidrogênio , Lipase/antagonistas & inibidores , Lipase/metabolismo , Modelos Moleculares , Estereoisomerismo , Especificidade por Substrato
2.
Med Arh
; 42(3): 119-24, 1988.
Artigo
em Servo-Croata (Latino)
| MEDLINE
| ID: mdl-3270752
3.
Med Arh
; 31(1): 25-7, 1977.
Artigo
em Servo-Croata (Latino)
| MEDLINE
| ID: mdl-865149
4.
Lijec Vjesn
; 94(7): 345-8, 1972 Jul.
Artigo
em Servo-Croata (Latino)
| MEDLINE
| ID: mdl-4657512
5.
Z Urol Nephrol
; 63(9): 668-73, 1970 Sep.
Artigo
em Alemão
| MEDLINE
| ID: mdl-5494388