RESUMO
The contraction kinetics of smooth muscle show a down-regulation after the transient rise found during sustained contraction. We tried to find out therefore if the contraction kinetics of rat tracheal smooth muscle can be re-accelerated during sustained activation. A 2 s length vibration (100 Hz sinusoidal; amplitude = 6% of the muscle length) produces an immediate fall in the force developed by the activated muscle. A biexponential function was fitted to the force recovery. The reciprocal of the time constant, t2, describing the slow component of force recovery, reflects the kinetics of contraction. The contraction kinetics reach their highest levels (t2 = 4.9 +/- 0.1 s,n = 166) about 30 s after the onset of electrical field stimulation. Three experimental groups were activated by either 10 microM serotonin (5-HT), 100 microM acetylcholine (ACh), or by 2 microM ACh for 50 min. Approximately 10 vibrations were applied to each preparation after an 8 min activation in order to observe stabilized down-regulated contraction kinetics. t2 values were calculated from the force recovery after vibration and averaged 11.2 +/- 0.2 s (n = 141), 11.5 +/- 0.2 s (n = 137), and 11.1 +/- 0.3 s (n = 84), respectively. After 50 min of continuous chemical activation, the preparation was stimulated additionally by the neurogenic release of acetylcholine. The t2 of post-vibration force recovery, as measured after 30 s of neural activation, showed no change in the specimens basically activated by 100 microM ACh (11.0 +/- 0.4 s, n = 51).(ABSTRACT TRUNCATED AT 250 WORDS)
