Can hydraulic design explain patterns of leaf water isotopic enrichment in C3 plants?

H2 18 O enrichment develops when leaves transpire, but an accurate generalized mechanistic model has proven elusive. We hypothesized that leaf hydraulic architecture may affect the degree to which gradients in H2 18 O develop within leaves, influencing bulk leaf stable oxygen isotope enrichment (ΔL ) and the degree to which the Péclet effect is relevant in leaves. Leaf hydraulic design predicted the relevance of a Péclet effect to ΔL in 19 of the 21 species tested. Leaves with well-developed hydraulic connections between the vascular tissue and the epidermal cells through bundle sheath extensions and clear distinctions between palisade and spongy mesophyll layers (while the mesophyll is hydraulically disconnected) may have velocities of the transpiration stream such that gradients in H2 18 O develop and are expressed in the mesophyll. In contrast, in leaves where the vascular tissue is hydraulically disconnected from the epidermal layers, or where all mesophyll cells are well connected to the transpiration stream, velocities within the liquid transport pathways may be low enough that gradients in H2 18 O are very small. Prior knowledge of leaf hydraulic design allows informed selection of the appropriate ΔL modelling framework.

The response of mesophyll conductance to short- and long-term environmental conditions in chickpea genotypes.

. Mesophyll conductance (g m) has been shown to vary between genotypes of a number of species and with growth environments, including nitrogen availability, but understanding of g m variability in legumes is limited. We might expect g m in legumes to respond differently to limited nitrogen availability, due to their ability to fix atmospheric N2. Using online stable carbon isotope discrimination method, we quantified genetic variability in g m under ideal conditions, investigated g m response to N source (N2-fixation or inorganic N) and determined the effects of N source and water availability on the rapid response of g m to photosynthetic photon flux density (PPFD) and radiation wavelength in three genotypes of chickpea (Cicer arietinum). Genotypes varied 2-fold in g m under non-limiting environments. N-fed plants had higher g m than N2-fixing plants in one genotype, while g m in the other two genotypes was unaffected. g m response to PPFD was altered by N source in one of three genotypes, in which the g m response to PPFD was statistically significant in N-fed plants but not in N2-fixing plants. There was no clear effect of moderate water stress on the g m response to PPFD and radiation wavelength. Genotypes of a single legume species differ in the sensitivity of g m to both long- and short-term environmental conditions, precluding utility in crop breeding programmes.

Stress-induced changes in carbon allocation among metabolite pools influence isotope-based predictions of water use efficiency in Phaseolus vulgaris.

Understanding how major food crops respond to environmental stress will expand our capacity to improve food production with growing populations and a changing climate. This study uses chemical and physiological adaptations to heat, water deficit and elevated light stresses in Phaseolus vulgaris L. to identify changes in carbon (C) allocation that, combined with post-photosynthetic fractionation of C isotopes, influences water use efficiency (WUE) predictions. The chemical stress response was explored through changes in C allocation to the carbohydrate and cyclitol pools using GC-triple quadrupole MS. Carbon allocation to the sucrose pool fluctuated significantly among treatments, and the putative osmolytes and osmoprotectants (myo-inositol and d-ononitol) accumulated under stress. Significant osmotic adjustment (P<0.05), quantified via pressure-volume curve analysis, was detected between control and stress treatments, although this was not attributable to active accumulation of the metabolites. Compound-specific 13C isotope abundance was measured using liquid chromatography isotope ratio MS to predict intrinsic WUE. In contrast to other metabolites measured, the δ13C of the sucrose pool fluctuated according to treatment and was proportional to predicted values based upon modelled Δ13C from gas exchange data. The results suggest that the accuracy and precision of predicting WUE may be enhanced by compound-specific analysis of Δ13C and that changes in the allocation of C among metabolite pools may influence WUE predictions based upon analysis of total soluble C. Overall, the plants appeared to use a range of mechanisms to cope with adverse conditions that could be utilised to improve plant breeding and management strategies.