[A CASE OF SEVERE AMLODIPINE POISONING].

The article is devoted to the actual problem--poisoning by calcium channel blockers. The case of the departure of the patient amlodipine, clinical picture, treatment and its effectiveness. In the discussion described pharmacological characteristics of the group of calcium channel blockers, mechanisms of development syndromes developing in this type of shipment and pathogenetic approach to therapy.

[Effect of the proteinase isolated from a fungus on the kallikrein-kinin system]. / Vliianie proteinazy, vydelennoi iz griba, na kallikrein-kininovuiu sistemu.

Carboxyl proteinase (CP) with the isoelectric point of 6.3-6.4 was isolated from a fungus at the Laboratory of Enzymology of the All-Union Research Technological Institute of Antibiotics and Enzymes and its effect on the kallikrein-kinin system and trypsin caseinolytic activity was studied. Four lots of the preparation with the activity of 1116 to 2300 milk coagulating units per 1 mg were used. The kininogenase activity of kallikrein, bradykinin and trypsin was determined with the routine biological procedures and the trypsin caseinolytic activity was determined with the Kunitz method and the diffusion method on casein-containing agar. It was shown that CP inhibited the kininogenase activity of kallikrein in the secretion of the salivary glands of man and crystalline trypsin in aqueous media and blood serum. It also inactivated the bradykinin constrictor effect on the smooth muscle tissue of the uterus horn in rats. CP had a capacity for inhibiting the caseinolytic activity of crystalline trypsin. Possible use of CP in treatment of patients with diseases accompanied by impairment of the kallikrein-kinin system (increased activity) is discussed.

[Mathematical modelling of the action of methotrexate on the kinetics of B-lymphocyte proliferation during the primary response]. / Matematicheskoe modelirovanie deistviia metotreksata na kinetiku proliferatsii B-limfotsitov pri pervichnom otvete.

A mathematical simulation model for checking hypotheses concerning specific features of methotrexate action on the primary antibody response is described. In general consistent interpretation of 8 experimental curves (an intact immune response and one when methotrexate was injected 72 hr after immunization; the data taken from literature) was obtained: the number of IgM-AbFCs in the mouse spleen in the first 4.5 days of immunization and the AbFC's labeling indices for three different 3H-thymidine injection schedules. It is concluded that methotrexate effects include the drug-induced lethality of the B-cells in G1-phase (early) and S-phase, S-phase blockage of the cell transfer cell retention on the G1/S boundary and also a decrease of the number of non-dividing IgM-AbFCs due to RNA and protein synthesis impairment.

[Mechanism of action of tetracycline on the functional state of the adrenal cortex]. / O mekhanizme deistviia tetratsiklina na funktsional'noe sostoianie kory nadpochechnikov

The mechanism of stimulation of the adrenal cortex function by tetracycline was studied on albino rats. It was shown that tetracycline administered orally in a dose of 200 mg/kg regularly induced an increase in the corticosterone levels in the peripheral blood of the animals by the 15th day of the antibiotic use. It was shown on the animals with an experimentally suppressed function of the hypophysis by prolonged administration of hydrocortisone acetate that tetracycline primarily stimulated the hypophysis function resulting in production and excretion of increased amounts of the adrenocorticotropic hormone into the blood. The hormone increased the production of corticosterone in the adrenal glands which resulted in its higher levels in the peripheral blood.

[Functional state of the adrenal cortex in the antibiotic therapy of experimental staphylococcal infection]. / Funktsional'noe sostoianie kory nadpochechnikov pri antibiotikoterapii éksperimental'noi stafilokokkovoi infektsii

The functional state of the cortex in experimental animals before and after treatment with tetracycline was studied under conditions of prolonged infection caused by intravenous administration of Staph. aureus. Three staphylococcal strains were used in the study: sensitive, low sensitive and resistant to tetracycline. It was shown that development of the infection in the animals was accompanied by a decrease in the cortex function. Oral administration of tetracycline in a dose of 200 mg/kg resulted in normalization of the glucocorticoid function of the gland after 15 days of its use. Since the same phenomenon was observed when the animals were infected with the antibiotic resistant strains of staphylococci, it was supposed that the therapeutic effect of tetracycline was defined by both the antibacterial action and capacity for increasing the protective-adaptive reactions of the host by stimulation of the cortex function.

[Study of the pharmacological properties and safety of a solution of levomycetin in hexamethylene tetramine for intravenous administration]. / Izuchenie farmakologicheskikh svoistv i bezvrednosti rastvora levomitsetina v geksametilentetramine dlia vnutrivennogo vvedeniia

Pharmacological properties of 2 per cent levomycetin solution in 40 percent hexamethylentetramine solution, as a new pharmaceutical form of levomycetin for intravenous administration prepared at drug-stores were studied. The maximum tolerating doses of the drug for mice, rabbits, and dogs were 26-47 times higher than the therapeutic ones with respect to the content of levomycetin and hexamethylentetramine. No increase in the toxicity of levomycetin and hexamethylentetramine in the preparation was observed. The drug in the doses 16 times higher than the therapeutic ones by the content of levomycetin did not almost change the arterial pressure and the drug in the doses 3.7 times higher than the therapeutic ones did not affect the blood coagulation either in acute experiments, or on its prolong intravenous infusion. Repeated administrations of the drug to rats and rabbits for 15-18 days in doses 3.7-4.8 times higher than the therapeutic ones by the content of levomycetin were innocuous for the animals. Absorption, circulation in the blood, distribution in the tissues and excretion with the urine of levomycetin used in the above pharmaceutical form did not differ from circulation of the antibiotic on its intravenous and oral administration. The drug is recommended for use in medical practice.

[Functional state of the adrenal cortex in experimental animals administered leyorin and its sodium salt perorally]. / Funktsional'noe sostoianie kory nadpochechnikov éksperimental'nykh zhivotnykh pri peroral'nom vvedenii levorina i ego natrievoi soli

The effect of oral levorin and its sodium salt on the function of the adrenal cortex was studied experimentally on albino rats. The functional activity of the adrenal cortex was tested by the content of corticosterone in the peripheral blood determined fluorometrically. The results showed that levorin in a dose of 300 000 Unit/kg and its sodium salt in doses of 300 000 and 1 200 000 Units/kg used orally induced at definite periods of their administration a decrease in the corticosterone blood levels. At the same time a decrease in the weight coefficients of the thymus and prostate was observed.