RESUMO
Eight novel HLA class I alleles detected by next-generation sequencing.
Assuntos
Alelos , Sequenciamento de Nucleotídeos em Larga Escala , Antígenos de Histocompatibilidade Classe I , Teste de Histocompatibilidade , Humanos , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Antígenos de Histocompatibilidade Classe I/genética , Antígenos de Histocompatibilidade Classe I/imunologia , Teste de Histocompatibilidade/métodos , Éxons/genética , Análise de Sequência de DNA/métodosRESUMO
Identification of two novel HLA alleles in Russian bone marrow donors.
Assuntos
Alelos , Doadores de Tecidos , Humanos , Federação Russa , Antígeno HLA-A2/genética , Antígeno HLA-A2/imunologia , Transplante de Medula Óssea , Antígeno HLA-B40/genética , Antígeno HLA-B40/imunologia , Éxons , Teste de Histocompatibilidade , Medula Óssea , Análise de Sequência de DNARESUMO
HLA-A*01:454 and HLA-A*31:229, two novel HLA-A alleles detected during routine typing by next-generation sequencing.
Assuntos
Alelos , Éxons , Antígenos HLA-A , Sequenciamento de Nucleotídeos em Larga Escala , Teste de Histocompatibilidade , Humanos , Antígenos HLA-A/genética , Análise de Sequência de DNA/métodos , Antígeno HLA-A1/genética , Sequência de BasesRESUMO
Five novel HLA-C alleles detected by next-generation sequencing: HLA-C*02:02:73, -C*03:04:106, -C*06:382, -C*07:1114Q and -C*12:408.
Assuntos
Alelos , Antígenos HLA-C , Sequenciamento de Nucleotídeos em Larga Escala , Teste de Histocompatibilidade , Humanos , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Antígenos HLA-C/genética , Teste de Histocompatibilidade/métodos , Éxons , Análise de Sequência de DNA/métodosRESUMO
Two novel HLA-DRB1 alleles were detected using next generation sequencing.
Assuntos
Alelos , Cadeias HLA-DRB1 , Sequenciamento de Nucleotídeos em Larga Escala , Teste de Histocompatibilidade , Humanos , Cadeias HLA-DRB1/genética , Éxons , Análise de Sequência de DNA/métodos , Sequência de BasesRESUMO
A novel HLA-C*07 allele, now officially designated HLA-C*07:02:150, was identified by next-generation sequencing.
Assuntos
Genes MHC Classe I , Antígenos HLA-C , Humanos , Antígenos HLA-C/genética , Alelos , Sequenciamento de Nucleotídeos em Larga EscalaRESUMO
Novel HLA-B*55:01:31, HLA-C*07:1113 alleles and confirmatory HLA-C*12:392 allele were detected during the HLA typing process.
Assuntos
Genes MHC Classe I , Antígenos HLA-C , Humanos , Alelos , Antígenos HLA-C/genética , Antígenos HLA-B/genética , Teste de HistocompatibilidadeRESUMO
We identified two novel HLA-DQB1 alleles by NGS, HLA-DQB1*03:519 and HLA-DQB1*06:01:35.
Assuntos
Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Alelos , Cadeias beta de HLA-DQ/genéticaRESUMO
Identification of the novel HLA-A*02:1148 and HLA-B*44:386 alleles by next-generation sequencing.
Assuntos
Antígenos HLA-A , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Alelos , Antígenos HLA-B/genéticaRESUMO
A novel HLA-B*35 allele, officially designated HLA-B*35:594, was identified by next-generation sequencing.
Assuntos
Alelos , Éxons , Sequenciamento de Nucleotídeos em Larga Escala , Teste de Histocompatibilidade , Humanos , Antígeno HLA-B35/genética , Análise de Sequência de DNA , Sequência de Bases , Códon , Antígenos HLA-B/genética , Doadores de TecidosRESUMO
We identified two novel HLA alleles by NGS, HLA-B*51:395 and HLA-DQB1*06:478.
Assuntos
Antígenos HLA-B , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Alelos , Cadeias beta de HLA-DQ/genética , Antígenos HLA-B/genéticaRESUMO
HLA-B*35:592 differs from HLA-B*35:03:01:01 by one nucleotide substitution in exon 4.
Assuntos
Genes MHC Classe I , Nucleotídeos , Humanos , Alelos , Sequência de Bases , Federação Russa , Análise de Sequência de DNARESUMO
Characterization of the novel HLA-C*07:1093 allele in a Russian female bone marrow donor.
Assuntos
Antígenos HLA-C , Nucleotídeos , Humanos , Feminino , Antígenos HLA-C/genética , Alelos , Teste de Histocompatibilidade , Éxons/genética , Doadores de TecidosRESUMO
We identified two novel HLA class II alleles by next-generation sequencing, HLA-DRB1*12:107 and HLA-DQB1*06:476.
Assuntos
Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Cadeias HLA-DRB1/genética , Frequência do Gene , Alelos , Haplótipos , Cadeias beta de HLA-DQ/genéticaRESUMO
Eighteen HLA allele sequences were confirmed and extended: 3 HLA-A, 6 HLA-B, 3 HLA-C, 2 HLA-DRB1, and 4 HLA-DQB1 alleles.
Assuntos
Medula Óssea , Antígenos HLA-A , Humanos , Alelos , Frequência do Gene , Antígenos HLA-A/genética , Haplótipos , Cadeias beta de HLA-DQ/genética , Antígenos HLA-B/genética , Cadeias HLA-DRB1/genética , Sistema de Registros , Federação RussaRESUMO
Detection of radiation-induced changes of the brain white matter is important for brain neoplasms repeated surgery. We investigated the influence of irradiation on the scattering properties of the white matter using optical coherence tomography (OCT). Healthy Wistar rats undergone the irradiation of the brain right hemisphere. At seven time points from the irradiation procedure (2-14 weeks), an ex vivo OCT study was performed with subsequent calculation of attenuation coefficient values in the corpus callosum followed by immunohistochemical analysis. As a result, we discovered acute and early-delayed changes characterized by the edema of different severity, accompanied by a statistically significant decrease in attenuation coefficient values. In particular, these changes were found at 2 weeks after irradiation in the irradiated hemisphere, while at 6- and 12-week time points they affected both irradiated and contralateral hemisphere. Thus, radiation-induced changes occurring in white matter during the first 3 months after irradiation can be detected by OCT.
Assuntos
Neoplasias Encefálicas , Substância Branca , Ratos , Animais , Substância Branca/diagnóstico por imagem , Ratos Wistar , Tomografia de Coerência Óptica/métodos , Neoplasias Encefálicas/diagnóstico por imagem , Encéfalo/diagnóstico por imagem , Encéfalo/efeitos da radiaçãoRESUMO
The new HLA-DRB1*11:320, HLA-DRB1*15:218, HLA-DQB1*05:324 alleles characterized in bone marrow donors.
Assuntos
Cadeias HLA-DRB1 , Humanos , Cadeias HLA-DRB1/genética , Frequência do Gene , Alelos , Haplótipos , Cadeias beta de HLA-DQ/genéticaRESUMO
Characterization of the novel HLA-C*07:1083 allele in a Russian bone marrow donor.
Assuntos
Genes MHC Classe I , Antígenos HLA-C , Humanos , Antígenos HLA-C/genética , Sequência de Bases , Alelos , Federação RussaRESUMO
Vaginal wall prolapse is the most common type of pelvic organ prolapse and is mainly associated with collagen bundle changes in the lamina propria. Neodymium (Nd:YAG) laser treatment was used as an innovative, minimally invasive and non-ablative procedure for the treatment of early-stage vaginal wall prolapse. The purpose of this pilot study was to assess connective tissue changes in the vaginal wall under prolapse without treatment and after Nd:YAG laser treatment using cross-polarization optical coherence tomography (CP OCT) with depth-resolved attenuation mapping. A total of 26 freshly excised samples of vaginal wall from 26 patients with age norm (n = 8), stage I-II prolapses without treatment (n = 8) and stage I-II prolapse 1-2 months after Nd:YAG laser treatment (n = 10) were assessed. As a result, for the first time, depth-resolved attenuation maps of the vaginal wall in the B-scan projection in the co- and cross-polarization channels were constructed. Two parameters within the lamina propria were target calculated: the median value and the percentages of high (≥4 mm-1) and low (<4 mm-1) attenuation coefficient values. A significant (p < 0.0001) decrease in the parameters in the case of vaginal wall prolapse compared to the age norm was identified. After laser treatment, a significant (p < 0.0001) increase in the parameters compared to the normal level was also observed. Notably, in the cross-channel, both parameters showed a greater difference between the groups than in the co-channel. Therefore, using the cross-channel achieved more reliable differentiation between the groups. To conclude, attenuation coefficient maps allow visualization and quantification of changes in the condition of the connective tissue of the vaginal wall. In the future, CP OCT could be used for in vivo detection of early-stage vaginal wall prolapse and for monitoring the effectiveness of treatment.
RESUMO
Photodynamic therapy (PDT) is a modern treatment for severe or treatment-resistant vulvar lichen sclerosus (VLS). The chronic and recurrent nature of VLS requires control of recurrences at an early stage. In this paper, a non-invasive multimodal optical coherence tomography (OCT) method was used to control for early histological signs of VLS recurrence after systemic PDT using Photodithazine®. To interpret the OCT data, a histological examination was performed before PDT and 3 months after PDT. Two groups of patients were identified: with early histological signs of VLS recurrence (Group I, n = 5) and without histological signs of VLS recurrence (Group II, n = 6). We use structural OCT, OCT angiography, and OCT lymphangiography throughout 6 months after PDT to visually assess the skin components and to quantitatively assess the dermis by calculating the depth-resolved attenuation coefficient and the density of blood and lymphatic vessels. The OCT data assessment showed a statistically significant difference between the patient groups 3 months after PDT. In Group II, all the studied OCT parameters reached maximum values by the 3rd month after PDT, which indicated recovery of the skin structure. At the same time, in Group I, the values of OCT parameters did not approach the values those in Group II even after 6 months. The obtained results of multimodal OCT can be used for non-invasive control of early histological recurrence of VLS after systemic PDT and for adjusting treatment tactics in advance, without waiting for new clinical manifestations of the disease.
