RESUMO
The restorative potential of trans-resveratrol (RV) was investigated in a rat neuronal cell line (PC12) exposed to organophosphate pesticide-monocrotophos (MCP). RV shows significant protection against MCP-induced alterations in PC12 cells by restoration of oxidative stress-mediated apoptosis and cytotoxicity. RV treatment significantly reduced reactive oxygen species (ROS) production and lipid peroxidation, and also restored glutathione levels and mitochondrial membrane potential, in cells receiving MCP. Restoration of markers such as cytochrome c, Bax, Bcl-2 and caspase-3 also confirms the effectiveness of RV against MCP-induced, mitochondria-mediated apoptosis in PC12 cells. The data identify the protective/restorative potential of RV against MCP-induced neuronal damages by affecting ROS production and the level of antioxidant defence enzymes.
RESUMO
Multi-walled carbon-nanotubes (MWCNTs)-induced apoptotic changes were studied in human lung epithelium cell line-A549. Non-cytotoxic doses of MWCNTs were identified using tetrazolium bromide salt (MTT) and lactate dehydrogenase (LDH) release assays. Cells were exposed to MWCNTs (0.5-100 µg/ml) for 6-72 h. Internalization and characterization of CNTs was performed by electron microscopy. Apoptotic changes were estimated by nuclear condensation, DNA laddering, and confirmed by expression of associated markers: p(53), p(21WAF1/CIP1), Bax, Bcl(2) and activated caspase-3. MWCNTs induced the production of reactive oxygen species and malondialdehyde along with significant decrease in the activity of catalase and glutathione. MWCNTs-induced ROS generation was found not to be associated with the mitochondrial activity. In general, the changes were significant at 10 and 50 µg/ml only. Results indicate the involvement of oxidative stress and apoptosis in A549 cells exposed to MWCNTs. Our studies provide insights of the mechanisms involved in MWCNTs-induced apoptosis at cellular level.
