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1.
J Forensic Sci ; 58(1): 90-100, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22994980

RESUMO

The stability of two benzisoxazole antipsychotics was determined in vitro in decomposing porcine blood inoculated with bacteria, utilizing a high-performance liquid chromatography with ultraviolet and fluorescence detection method for drug quantitation. Stability experiments for risperidone and paliperidone were conducted at 7, 20 and 37°C for 4 days using sterile and bacterially inoculated porcine blood. The drugs were stable in sterile blood at each temperature and in inoculated blood at 7°C, but degraded significantly in inoculated blood at 20 and 37°C. Complete loss occurred within 2 days when incubated at 37°C. The benzisoxazole-cleaved degradation products for both drugs were identified as 2-hydroxybenzoyl-risperidone and 2-hydroxybenzoyl-paliperidone utilizing liquid chromatography quadrupole-time-of-flight mass spectrometry and accurate mass measurements. The degradation products have been found in postmortem case studies, including one case where risperidone and paliperidone were not detected, indicating complete conversion can occur in situ.


Assuntos
Antipsicóticos/sangue , Bactérias Gram-Positivas/metabolismo , Isoxazóis/sangue , Mudanças Depois da Morte , Pirimidinas/sangue , Risperidona/sangue , Animais , Cromatografia Líquida de Alta Pressão/métodos , Estabilidade de Medicamentos , Toxicologia Forense , Palmitato de Paliperidona , Risperidona/análogos & derivados , Manejo de Espécimes , Espectrometria de Massas por Ionização por Electrospray , Suínos , Temperatura
2.
J Forensic Sci ; 58 Suppl 1: S117-25, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22931374

RESUMO

It is well established that bacteria are capable of degrading selected drugs during decomposition. The aim of this study was to investigate the stability of several serotonin-selective reuptake inhibitor antidepressants and venlafaxine during putrefaction in porcine liver macerate inoculated with porcine cecal contents rich in bacteria. Blank liver matrices, sterile liver macerates, and sterile aqueous controls were included with the experiment performed for 57 days at 20°C under anaerobic conditions. A liquid chromatography/mass spectrometry method was developed for quantitative determination of the drugs investigated in both sterile and decomposed liver matrices. The method was found to encounter matrix effects not detected during the validation stage. Citalopram, paroxetine, sertraline, venlafaxine, and fluoxetine were found to be stable under the experimental conditions; however, fluvoxamine was found to be decreased by c. 50% over 57 days in bacterially inoculated liver macerate. This study suggests that fluvoxamine concentrations in cases with evidence of decomposition/putrefaction should be interpreted with extra caution.


Assuntos
Fígado/química , Mudanças Depois da Morte , Inibidores Seletivos de Recaptação de Serotonina/análise , Animais , Cromatografia Líquida , Estabilidade de Medicamentos , Toxicologia Forense , Fígado/microbiologia , Espectrometria de Massas , Inibidores Seletivos de Recaptação de Serotonina/química , Suínos
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