Histochemical and morphological changes in various regions of the rat hippocampus in swimming-induced stress.

Swimming stress increased the functional activity of hippocampal pyramidal neurons in rats, as indicated by decreases in their glycogen contents and increases in their nucleic acid contents and the nucleus:cytoplasm ratio. These changes were most marked in hippocampal field CA1, while changes in other regions of the hippocampus were minimal.

[Swimming stress-induced histochemical and morphological changes in various regions of the rat hippocampus]. / Gistokhimicheskie i morfologicheskie izmeneniia v razlichnykh oblastiakh gippokampa krys pri plavatel'nom stresse.

In rats, swimming stress enhanced functional activity of the hippocampus' pyramidal neurones as manifested by diminishing of the glycogen contents, increasing amount of nucleic acids, augmented nucleus/cytoplasm ratio.

[Histochemical and morphometric evidence of the participation of dorsal hippocampal neurons in the antistress effect of melatonin]. / Gistokhimicheskie i morfometricheskie dokazatel'stva uchastiia neironov dorsal'nogo gippokampa v antistressornom deistvii melatonina.

The epiphyseal hormone melatonin (0.1 mg/kg) does not influence the functional state of pyramidal neurons in the dorsal hippocamp of intact rats, but decreases the swimming-stress-induced activation of these neurons. Removal of the pineal gland increased the functional activity of neurons in the intact rats and potentiated the stress-induced histochemical and morphometric changes.

[Effect of diazepam on the metabolic and morphometric parameters of neurons in the dorsal hippocampus in normal and stressed rats]. / Vliianie diazepama na metabolicheskie i morfometricheskie kharakteristiki neironov dorsal'nogo gippokampa u normal'nykh i podvergnutykh stressu krys.

Diazepam (0.5 mg/kg) decreases the swimming-stress-induced activation of the pyramidal neurons in the dorsal hippocamp. This is manifested by a decrease in the stress-induced glycogen consumption, an increase in the RNA content, and (less pronounced) increased in the nucleocytoplasmic ratio.

[The functional metabolic activity of the blood neutrophils in different clinical forms of brucellosis]. / Funktsional'no-metabolicheskaia aktivnost' neitrofilov krovi pri raznykh klinicheskikh formakh brutselleza.

The characteristics of the activity of alkaline phosphatase, nonspecific esterase, myeloperoxidase, lysosomal cation proteins, lipids have been determined in the cytochemical study of neutrophils in peripheral blood samples of 127 patients with acute, chronic, primary chronic brucellosis and osteochondrosis. It is expedient to use these characteristics as additional tests for the differential diagnostics of different clinical forms of brucellosis, as well as residual brucellosis and osteochondrosis.

[Natural resistance factors in brucellosis]. / Nekotorye faktory estestvennoi rezistentnosti pri brutselleze.

A higher level of the opsono-cytophagous capacity of the blood and the quantitative content of lysosomal cationic proteins in polymorphonuclear neutrophils in the blood of Fischer rats in comparison with the corresponding characteristics in normal guinea pigs has proved to be conductive to more active elimination of the infective agent from the body of the animals after their infection with brucellosis. The indices characterizing the activity and intensity of the phagocytosis of brucellae by polymorphonuclear neutrophils of the blood in combination with the determination of the amount of cationic proteins in these cells permit the objective characterization of the level of nonspecific resistance to brucellosis.

[Cytochemical changes in the peripheral blood leukocytes of guinea pigs inoculated against plague, tularemia and anthrax]. / Tsitokhimicheskie izmeneniia v leikotsitakh perifericheskoi krovi u morskikh svinok, privitykh protiv chumy, tuliaremii i sibirskoi iazvy.

The immunization of guinea pigs with trivaccine and monovaccines against plaque, tularemia and anthrax induces a decrease in the activity of acidic phosphatase in lymphocytes, as well as a decrease in the number of lymphocytes containing this enzyme. A decrease in the activity of alkaline phosphatase and peroxidase had been found to occur in neutrophil leukocytes. Besides, neutrophil leukocytes have shown an increase in the activity of acidic phosphatase and nonspecific esterases. The study based on the evaluation of the activity of the above-mentioned enzymes in lymphocytes and neutrophils has not revealed the predominant influence exercised by any of the antigens, different in their nature and used separately or in the form of a combined preparation, on immunogenesis.

[Comparative cytoenzymatic study of infectious and vaccinal processes in experimental brucellosis]. / Sravnitel'noe tsitoénzimaticheskoe issledovanie infektsionnogo i vaktsinnogo protsessov pri éksperimental'nom brutselleze.

In experiments on 240 guinea-pigs metabolic changes were found to occur in peripheral blood cells in the process of the development of brucellosis infection and after immunization. The degree and character of the activity of lysosomal enzymes depended on the time of infection and immunity formation. In comparison with the vaccinal culture of Br. abortus 19, the virulent culture of Br. abortus 544 induced greater changes in the activity of esterase, acidic and alkaline phosphatase in neutrophils, in the activity of acidic phosphatase in lymphocytes, as well as in the number of lymphocytes containing this enzyme. The data on enzymatic activity are recommended for use as a differential test for evaluating the character of the infectious and vaccinal processes.

[Disinfecting effect of fixatives in histoenzymatic analysis of Vibrio cholerae]. / Obezzarazhivaiushchee deistvie fiksatorov pri gistoenzimaticheskikh issledovaniiakh kholernogo Vibriona.

Data are presented on the disinfecting action of fixative fluids on the cholera causative agents in the freshly frozen sections from the tissues and organs of animals infected with cholera. The results obtained offered a possibility of drawing a conclusion on the harmlessness of the histoenzymatic studies on the material infected with cholera vibrio.