RESUMO
Repair of thermal injury of Physarum polycephalum Schw. plasmodia has been studied by light and electron microscopy. As a result of heating the plasmodia for 10 min at 42°C both the unordered and shuttle protoplasmic streaming were arrested; the outer plasmodial membrane showed alterations at sites of contact with water; the onset of the next mitosis was considerably delayed. The plasmodial ultrastructure was markedly disturbed, including disappearance of the granular component of the nucleoili and a compact, almost fibrillar structure of the latter. The mitochondria became distorted and their intracristal spaces enlarged while the outer and inner membranes appeared in some places to be separated. Glycogen particles disappeared from the cytoplasm. Recovery of both types of protoplasmic streaming of the motility of the plasmodium, of the resistance of its membrane to contact with water, and of the ability of the organism to go through the cell cycle went all hand in hand with the normalization of the structure of nucleoli, mitochondria and cytoplasm. All of the functional and structural characteristics are normalized within ca. 9 h following heating.
RESUMO
The thermostabilities of the "unordered" and shuttle protoplasmic streamings in myxomycete Physarum polycephalum plasmodia was studied. A comparison of these thermostabilities has revealed that the cessation of the former streaming occurs at temperatures higher than those required for arresting the shuttle streaming. The difference between the two types of protoplasmic streamings is better seen in the rate of repair of protoplasmic streaming halted by a 10 minutes heating at 38-41 degrees C. For example, the unordered streaming is restored 2 minutes after heating plasmodia at 39 degrees for 10 min., while the shuttle streaming is resumed in 24 minutes. It is supposed that the two protoplasmic streamings are independent to an appreciable extent, and that the shuttle streaming, being more complex and coordinated, has appeared in the evolution at later stages than the unordered one. The higher heat sensitivity of the shuttle streaming substantiates a view of the lower stability to injury in regulatory mechanisms if compared to the stability of motile mechanisms.
Assuntos
Aclimatação , Corrente Citoplasmática , Temperatura Alta , Mixomicetos/fisiologia , Physarum/fisiologia , AnimaisRESUMO
It has been shown that phototaxis of chloroplasts may be used as a criterion of the degree of injury in leaf parenchyma of many plant species. This method was applied here to determine heat resistance of leaf parenchyma, its resistance to ethanol and high hydrostatic pressure and also to characterize reversibility and heat hardening.
RESUMO
The leaves of Tradescantia fluminensis VELL, were kept in light and darkness after short-term heating (5 min and 10 min) at different temperatures. In light temperature causing injury was 10° lower than in darkness. A considerable destruction of chlorophyll occurred when the heated leaves were kept in light. If the light intensity was 4,000 lux or even lower the damage to cells was not accompanied by bleaching of chlorophyll. Light produced no effect on unheated leaves. In variegated white-green leaves of Chlorophytum elatum R. BR. light injured only green parts of leaf blades. The minimal light intensity which brought about injury of Tradescantia leaves in experimental conditions was 1,000 lux. Light of the same intensity accelerated death of heated isolated leaves of Cucumis sativus L.Light damage to Tradescantia leaves occurred when the action of light was accompanied by that of high temperature.In an atmosphere of nitrogen the injurious effect of light sharply decreased.It is suggested that the injury of heated leaves in light is caused by photooxydation which is sensitized by chlorophyll and occurs at the expense of photochemical energy which is not used in photosynthesis. Photosynthesis itself is repressed by heating.
