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1.
Animals (Basel) ; 13(22)2023 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-38003076

RESUMO

Avian malaria is a re-emerging threat to avian species worldwide. It is sustained by several protozoan species belonging to the genus Plasmodium, mainly Plasmodium relictum. The even wider diffusion of the disease, probably because of the increase in the areas covered by their mosquito vectors, may pose new risks for avian species lacking natural resistance (especially those from artic or sub-artic environments) or those hosted in structures like zoos and wildlife rescue centers. With that premise, this study describes the efficacy and safety of a therapeutic protocol to treat avian malaria in three snowy owls (Bubo scandiacus) hosted in a wildlife rescue center in Apulia, south of Italy, and affected by avian malaria by P. relictum. The protocol consisted of administering 10/4 mg/kg atovaquone/proguanil per os once a day for three consecutive days, repeating this seven days later. Seven days after the end of the treatment, P. relictum was not detected in the birds' blood and no adverse effects were observed during the 60 days of monitoring after the end of the treatment. Therefore, a therapeutic regimen of 10/4 mg/kg/day may be considered safe and effective in a valuable and endangered species such as B. scandiacus.

2.
Microbiol Spectr ; : e0249423, 2023 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-37728570

RESUMO

Starting from June 2022, increased mortality associated with enteric signs was reported in European hedgehogs (Erinaceus europaeus) recovered at a regional wildlife rescue center, in Apulia, Italy. Cases of enteric disease were observed until the end of the breeding season, despite increased biosafety measures. A novel parvovirus was identified using metaviromic, and parvovirus-like particles were observed in the stools on electron microscopy observation. The virus was detected in the fecal samples of all the animals tested (n = 9) and in the internal organs (liver, spleen, and kidney) of three out of nine animals using a specific quantitative assay. In the full-length genome, the parvovirus was closely related (90.4% nt) to a chaphamaparvovirus identified in an Amur hedgehog (Erinaceus amurensis) in Asia and to chaphamaparvoviruses (≤ 70% nt) detected in bats and rodents. Since chaphamaparvoviruses are considered as pathogen in rodents, it will be important to investigate the pathogenic role, if any, of these parvoviruses in hedgehogs. IMPORTANCE European hedgehogs (Erinaceus europaeus) are common in Europe. This species has been shown to harbor occasionally zoonotic pathogens, including bacteria, fungi, and viruses. Exploring the virome of wildlife animals is important for animal conservation and also to assess zoonotic risks. Our metaviromic investigation identified a novel parvovirus from an outbreak of enteritis in European hedgehogs housed in a wildlife rescue center, extending the spectrum of potential viral pathogens in this species.

3.
Animals (Basel) ; 13(16)2023 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-37627430

RESUMO

Colibacillosis, caused by E. coli, is responsible for economic losses in the poultry industry due to mortality, decreased production, and the cost of antibiotic treatments. Prevention of colibacillosis is based on improved biosecurity measures and the use of the vaccine performed with O78 E. coli strains, which is responsible for most cases of colibacillosis. Recently, there has been increased interest in other infection control methods, such as the use of natural compounds. The aim of this study was to evaluate the antimicrobial efficacy of cinnamon essential oil (CEO) against E. coli strains isolated from poultry. The MIC50 and MIC90 of CEO were determined by testing 117 strains belonging to serogroups O78, O2, O128, O139, isolated from laying hens (91 strains), broilers (10 strains), and turkeys (16 strains). The bacterial strains were tested at cell densities of 108 and 106 CFU/mL. At the cell density of 108 CFU/mL, MIC50 and MIC90 were 0.4 and 0.5 µL/mL for most of the tested strains, while they corresponded to 0.5 µL/mL for all strains isolated from broilers and for strains belonging to serogroup O139. At the cell density of 106 CFU/mL, MIC50 and MIC90 were 0.3 and 0.4 µL/mL, regardless of bird species of origin and for strains belonging to serogroups O78 and O2. In addition, a concentration of 0.04 µL/mL of CEO corresponded both to MIC50 and MIC90 for strains belonging to serogroups O139 and O128. Based on these results, cinnamon essential oil showed an effective antibacterial activity against E. coli strains from poultry and could find field application for the prevention of colibacillosis.

4.
Animals (Basel) ; 12(20)2022 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-36290276

RESUMO

Climate change, excessive exploitation of agricultural land which reduces natural habitats, wildlife shooting, and the use of pesticides all cause difficulties for wildlife, with considerable numbers of animals being brought to wildlife rescue centres. Although the efforts of staff involved in wildlife management at these centres usually focus on therapeutic treatments to reintroduce them into the wild, the monitoring of pathogens that may be transmitted to humans is of relevance. Campylobacter (C.) jejuni and C. coli are frequently carried by animals without inducing clinical signs and are responsible for enteric disorders and more rarely extra-intestinal disease in humans. Farm species and poultry, in particular, are the main reservoirs of C. jejuni and C. coli, but wild animals may also be carriers. The aim of this paper was to investigate the presence of C. jejuni and C. coli in wild birds housed at a wildlife rescue centre and to evaluate the sensitivity of the detected strains to antibiotics. Campylobacter was found in 52 out of 209 (24.88%) birds from 33 different species. C. jejuni was more prevalent, while C. coli was only detected in three Long-eared Owls (Asio otus). The incidence of the infection was particularly high (72.22%) among omnivorous species. Infection rates were higher in birds housed indoors (57.14%) than outdoors (31.74%). Moreover, Campylobacter was not detected in species whose mean temperature body is below 40 °C or higher than 42.2 °C. The most common antibiotic resistance in the tested strains was against trimethoprim/sulfamethoxazole, ciprofloxacin and enrofloxacin. In addition, multi-drug resistance was also found. The results highlight the need to increase biosecurity measures at rescue centres so as to reduce health-related risks to workers involved in wildlife management.

5.
Vet Sci ; 9(6)2022 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-35737353

RESUMO

The number of pets such as dogs, cats, rabbits, and parrots has increased in European families. Social benefits to owners such as decreasing feelings of loneliness and anxiety are provided by pets which are also used in Animal-assisted Therapy (AAT). Nevertheless, human-animal interactions are also associated with health problems including allergies, asthma, and zoonosis. Rabbits may carry potential pathogens for humans. One of the most common bacteria that colonizes the oro-pharynx and the upper respiratory tract of rabbits is Pasteurella (P.) multocida. Transmission of the infection to humans results from scratches, licks, and bites but it also can occur from the inhalation of air particles containing the microorganism. Immunocompromised people or persons with pulmonary disorders are particularly susceptible to the infection. Infected rabbits may carry P. multocida with or without clinical signs. In this paper, the sensitivity to antibiotics and the invasiveness ability of P. multocida identified in a farm of pet rabbits affected by severe pasteurellosis were investigated. The strain was P. multocida belonging to capsular type A which is the type most often detected in humans. The identified strain was susceptible to the tested antibiotics, but it appeared equipped with several virulence genes which are responsible for fimbriae production, adhesion processes to host cells, enzyme production, and are involved in iron acquisition processes. These findings are of particular interest because rabbits recovered from pasteurellosis very often become carriers of the bacteria. Therefore, we suggest considering P. multocida screening in the routine medical checks of rabbits, especially if they are meant to be companion animals for children and elder people, given that the transmission of the pathogen cannot be excluded.

6.
Animals (Basel) ; 11(6)2021 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-34073932

RESUMO

Monitoring of infections that may be transmitted to humans by animals in wildlife rescue centres is very important in order to protect the staff engaged in rehabilitation practices. Salmonella may be a natural inhabitant of the intestinal tract of turtles, rarely causing disease. This may represent a potential risk for humans, increasing the sanitary risk for operators in wildlife rescue centres. In this paper, the occurrence of non-typhoidal Salmonella among terrestrial turtles housed in a wildlife rescue centre in Southern Italy was investigated, in order to assess the serovars more frequently carried by turtles and identify those that may represent a risk for operators involved in wildlife management. Sixty-nine adult turtles (Testudo hermanni hermanni, T. h. boettgeri, T. graeca, and T. marginata) were tested. Detection and serotyping of Salmonella strains were performed according to ISO 6579-1 and ISO/TR 6579-3:2013, respectively. The distribution of Salmonella spp. was significantly higher in T. hermanni hermanni than in other species, independent of the age and gender of the animals. Two different Salmonella species, S. enterica and S. bongori, three S. enterica subspecies (enterica, diarizonae, salamae), and five different serovars (Hermannswerder, Abony, Ferruch, Richmond, Vancouver) within the group S. enterica subspecies enterica were identified. Different combinations of Salmonella types were simultaneously found in specimens of T. h. hermanni. Most of detected Salmonella types may represent a potential risk for public health. Adopting correct animal husbandry procedures and informing on potential sanitary risks may be useful for minimising the risk of transmission of Salmonella to workers involved in wildlife management.

7.
Animals (Basel) ; 11(4)2021 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-33916753

RESUMO

Large numbers of wild animals are injured every year in road traffic accidents. Scant data are available for rescued wild carnivores, in particular for red foxes. Cases of foxes with head trauma were retrospectively considered for inclusion in this study. Clinical examination, modified Glasgow coma scale (MGCS), computed tomography (CT) examination, therapy, outcome, and post mortem findings of the brain were investigated. In all foxes, cranial vaults lesion occurred in single (67%) or multiple sites (33%). Midline shift and hydrocephalus were observed in this population. The mean survival was 290 (±176) days. In our study, we performed CT scans on average 260 days after fox rescue, and we speculate that persisting clinical signs could be attributed to TBI. In our study, only two foxes were alive at the time of writing. Other foxes were euthanized due to the severity of the clinical signs. CT scans help diagnose chronic lesions and their effect on prognostic judgment for animals released to wildlife environments.

8.
Minerva Stomatol ; 68(5): 236-241, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31822047

RESUMO

BACKGROUND: In recent years, we have recorded an increase in the number of hospital admissions due to odontogenic abscesses and neck phlegmons requiring surgery. We believe that the cause may be related both to the rationalization of funding allocation procedures in public health service making access to public dental healthcare more difficult, and the financial situation of Italian families. METHODS: Fifty-five patients admitted between January 2013 and November 2017 with a diagnosis of "neck abscess" and/or "neck phlegmon" and/or "neck infection" were enrolled in this longitudinal retrospective study. RESULTS: The results of this study showed that the number of cases of abscesses and neck phlegmons with an odontogenic origin have quadrupled, with reference to gender was 31 males (56%) and 24 females (44%). The average age was 46 and most cases involved patients between 30 and 50. Eighteen patients (33%) were not EU citizens and had been living in Italy for over 5 years. These data indicate an increase in the incidence of odontogenic neck infections. The data gathered by ISTAT (Istituto Nazionale di Statistica), Eurostat Data Explorer, Fondazione Giuseppe Di Vittorio and Tecnè revealed that these outcomes are directly related to the financial difficulties Italian families are experiencing. The exclusion of essential dental assistance from public healthcare forced Italians to consult expensive private facilities that not everyone can afford. CONCLUSIONS: Connections were found between odontogenic neck infection complications and the financial difficulties Italian families are suffering from; Italians are reducing their budget for dental healthcare becoming exposed to serious neck pathologies.


Assuntos
Abscesso , Celulite (Flegmão) , Feminino , Humanos , Itália , Masculino , Pessoa de Meia-Idade , Pescoço , Estudos Retrospectivos
9.
Animals (Basel) ; 9(12)2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31779284

RESUMO

Any human activity, even if aimed at the improvement of a natural area, can potentially affect wildlife, leading to possible short-term or long-term changes due to the human-wildlife interaction. In this study, a botulism outbreak which occurred in waterfowl in a nature reserve after a conservative environmental action is reported. More than 180 different species of wild birds, including seventy waterfowl species, live in the area. The wildlife reserve rangers built an artificial pond equipped with draining canals in the wetland in order to improve the environment of waterfowl species and to facilitate their supply of food. Then, presumably due to tidal rides, gray mullets (Mugil cephalus) arrived from the sea and settled in the pond. The number of fishes gradually increased, and several fishes died with a peak of mortality in the summer of 2017, creating a great amount of decaying organic material and the optimal conditions for Clostridium botulinum growth and toxin production. A botulism outbreak then occurred rapidly and was characterised by flaccid paralysis and sudden mortality of the birds. Seven mallard ducks (Anas platyrhynchos), 4 common teals (Anas crecca), 1 garganey (Anas querquedula), 2 wood sandpipers (Tringa glareola), 1 little egret (Egretta garzetta), 1 little grebe (Tachybaptus ruficollis), and 4 Eurasian coots (Fulica atra) were found dead. Interestingly, the toxin identified as responsible for the disease outbreak was the mosaic of type C and D toxins (C/D type). The prompt removal of the fish carcasses led to a rapid resolution of the outbreak of the disease, highlighting the relevance of a correct management for any action in environmental contexts. The conclusion is that any human activity in wildlife habitats should be carefully considered in order to assess the possible impacts and to quickly identify the possible risks of changes in wildlife population.

10.
Artigo em Inglês | MEDLINE | ID: mdl-30011925

RESUMO

Biological risk assessment in occupational settings currently is based on either qualitative or semiquantitative analysis. In this study, a quantitative microbial risk assessment (QMRA) has been applied to estimate the human adenovirus (HAdV) health risk due to bioaerosol exposure in a wastewater treatment plant (WWTP). A stochastic QMRA model was developed considering HAdV as the index pathogen, using its concentrations in different areas and published dose⁻response relationship for inhalation. A sensitivity analysis was employed to examine the impact of input parameters on health risk. The QMRA estimated a higher average risk in sewage influent and biological oxidation tanks (15.64% and 12.73% for an exposure of 3 min). Sensitivity analysis indicated HAdV concentration as a predominant factor in the estimated risk. QMRA results were used to calculate the exposure limits considering four different risk levels (one illness case per 100, 1.000, 10.000, and 100.000 workers): for 3 min exposures, we obtained 565, 170, 54, and 6 GC/m³ of HAdV. We also calculated the maximum time of exposure for each level for different areas. Our findings can be useful to better define the effectiveness of control measures, which would thus reduce the virus concentration or the exposure time.


Assuntos
Poluentes Ocupacionais do Ar/análise , Exposição por Inalação/análise , Exposição Ocupacional/análise , Eliminação de Resíduos Líquidos/métodos , Águas Residuárias/virologia , Humanos , Exposição por Inalação/prevenção & controle , Modelos Estatísticos , Exposição Ocupacional/prevenção & controle , Medição de Risco , Sensibilidade e Especificidade , Viroses/prevenção & controle , Eliminação de Resíduos Líquidos/normas
11.
Aging Clin Exp Res ; 29(3): 419-426, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27324693

RESUMO

BACKGROUND: Aging is characterized by a physiological reduction in physical activity, which is inversely correlated with survival. AIMS: Aim of the present study is to evaluate the cardiovascular, central hemodynamic and autonomic responses to a single bout of adapted physical exercise in octogenarian subjects. METHODS: We studied cardiovascular, hemodynamic and autonomic responses to adapted physical activity in 33 subjects by a noninvasive methodology (Nexfin®, Edwards Lifesciences Corporation). RESULTS: Our octogenarians presented a significant increase in mean arterial pressure (p < 0.01) and heart rate (p < 0.005) in response to exercise, while both are reduced during the early recovery phase. Central hemodynamic showed a significant increase in stroke volume (p < 0.05), cardiac output (p < 0.01) and left ventricle contractility index (p < 0.01), whereas systemic vascular resistance showed a significant decrease (p < 0.001). We found a reduction in baroreflex control of the sinus node during exercise. DISCUSSION: Our data demonstrate that in very old people adapted physical activity is able to activate cardiovascular system and to induce a postexercise hypotension similarly to adults. The baroreflex control of sinus node seems to contribute in the physiological mechanism of these cardiovascular adaptations. CONCLUSIONS: In very old people, physical activity induces cardiovascular and hemodynamic responses not significantly different from those induced in adult even though some cautions particularly in the early recovery phase after exercise should be exercised.


Assuntos
Envelhecimento/fisiologia , Sistema Nervoso Autônomo/fisiologia , Sistema Cardiovascular , Exercício Físico/fisiologia , Frequência Cardíaca/fisiologia , Idoso , Idoso de 80 Anos ou mais , Barorreflexo/fisiologia , Pressão Sanguínea/fisiologia , Feminino , Humanos , Masculino , Volume Sistólico/fisiologia
12.
Am J Infect Control ; 36(4): 304-7, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18455053

RESUMO

BACKGROUND: Many dental procedures produce extensive aerosols and splatters that are routinely contaminated with microorganisms. METHODS: Air containing blood-bearing aerosols and surfaces contaminated by sedimenting blood particulate was sampled in 5 different dental cubicles. To assess contamination by blood particulate, the concentration of hemoglobin (Hb) in the air and on the sedimentation surfaces was determined. RESULTS: The mean concentration of Hb in the air aspirated in the 5 cubicles was 0.14 +/- 0.23 microg/m(3), corresponding to a blood volume of 8.7 x 10(-4) microL/m(3). Similarly, the mean concentration of blood particulate sedimented on surfaces was calculated and found to be 1.56 microL/m(2). In 80% of the cubicles monitored, 100% positivity to the Hb determination test was recorded in all of the surface samples. CONCLUSIONS: The results obtained revealed contamination of both air and surfaces by blood particulate. Moreover, with the exception of those obtained in 1 cubicle, all of the samples of sedimenting particulate analyzed were positive for the presence of Hb.


Assuntos
Aerossóis/análise , Clínicas Odontológicas , Odontologia , Contaminação de Equipamentos , Hemoglobinas/análise , Controle de Infecções , Sangue , Monitoramento Ambiental , Fidelidade a Diretrizes , Humanos , Transmissão de Doença Infecciosa do Paciente para o Profissional/análise , Itália , Exposição Ocupacional/análise , Material Particulado/análise , Guias de Prática Clínica como Assunto , Fatores de Risco
13.
Int J Environ Health Res ; 18(1): 73-8, 2008 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-18231947

RESUMO

The potential harmful effects of glutaraldehyde on human health are well known, and in recent years various new substitutes for this compound have been proposed for the disinfection and thorough sterilization of medical instruments. Nevertheless, glutaraldehyde is still widely used in hospital environments. In order to evaluate environmental contamination by glutaraldehyde vapours, the rooms of a hospital out-patient department of digestive endoscopy were monitored in 2005; a total of 52 samples were taken. The mean environmental concentration of glutaraldehyde was 3.7+/-7.4 microg/m(3). The number of efficacious air exchanges per hour was 6.3 v/h in all of the environments monitored. The study revealed that, in a hospital setting, adequate structural and functional planning, combined with responsible management on the part of the personnel and constant careful checking of the results obtained can minimize the risk of occupational exposure to glutaraldehyde.


Assuntos
Poluição do Ar em Ambientes Fechados/análise , Desinfetantes/análise , Monitoramento Ambiental/métodos , Glutaral/análise , Endoscopia do Sistema Digestório , Arquitetura Hospitalar , Humanos , Itália , Exposição Ocupacional/análise , Ambulatório Hospitalar
14.
Acta amaz ; 38(1): 77-84, 2008. graf, mapas, tab
Artigo em Português | LILACS | ID: lil-482509

RESUMO

O objetivo do presente artigo foi o de avaliar o produto MOD14 quanto à sua performance para detectar áreas queimadas na Amazônia, mais específicamente no norte do Estado do Mato Grosso, considerando o período entre 21/06/2004 e 07/07/2004, comparando os resultados fornecidos pelo MOD14 e interpretações visuais de áreas queimadas obtidas de imagens TM/Landsat-5. O produto MOD14 detectou 51,58 por cento do total de áreas queimadas, cujo tamanho médio foi de 83,14 ha; os restantes 48,42 por cento não detectados tinham tamanho médio de 38,74 ha. O produto MOD14 superestima a quantidade de áreas queimadas ao classificar solos expostos, florestas e outros tipos de coberturas da terra como pixels "hot spots"; além disso, este produto não detecta áreas queimadas de dimensões pequenas (< 100 ha), proporcionando erros de classificação em grandes áreas de solos expostos.


The main objective of this paper was to evaluate MOD14 product performance to detect burned areas in Amazonia, more specifically in the north of Mato Grosso State (Brazil), considering the period of 06/21/2004 to 07/07/2004, by comparing the results provided by MOD14 product with visual discrimination of burned areas using TM/Landsat-5 images. MOD14 product detected 51.58 percent of the total burned areas, which have an average size of 83.14 ha; the remaining 48.42 percent undetected burned areas have an average size of 38.74 ha. MOD14 product overestimates the burned areas by classifying exposed soils, forests and other types of land cover as hot pixels. In addition MOD14 product does not detect small burned areas (< 100 ha), generating classification errors in large areas of exposed soils.


Assuntos
Algoritmos , Conservação dos Recursos Naturais , Tecnologia de Sensoriamento Remoto
15.
Am J Infect Control ; 34(5): 264-8, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16765203

RESUMO

A total of 1,030 microbiological samples were taken in 3 hospital wards with different air-conditioning features: no conditioning system (ward A), a conditioning system equipped with minimum efficiency reporting value (MERV) filters (ward B), and a conditioning system thoroughly maintained and equipped with high-efficiency particulate air (HEPA) filters (absolute) (ward C). The air in each ward was sampled, and the bacterial and fungal concentrations were determined by active and passive methods. The concentration of fungi on surfaces was also determined. Active sampling showed positive samples in wards A and B only, with average values of 0.50 colony-forming units (CFU)/m(3) (95% CI, 0.30 to 0.70) in A and 0.16 CFU/m(3) (95% CI, 0.13 to 0.20) in B. Passive sampling was positive only in ward A (mean, 0.14 CFU/cm(2)/h; 95% CI, 0.13 to 0.15). Aspergillus was found in 27% and 22% of sampled surfaces in wards A and B, respectively, but in no samples from ward C. The most commonly found species was A. fumigatus (76% of cases in A and 34% of cases in B). The results show that the use of air-conditioning systems markedly reduces the concentration of aspergilli in the environment. Proper maintenance of these systems is clearly fundamental if their efficacy is to be ensured.


Assuntos
Ar Condicionado , Poluentes Atmosféricos/isolamento & purificação , Unidades Hospitalares , Aspergillus/isolamento & purificação
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