RESUMO
OBJECTIVE: To study the chemical constituents from the leaves of "Chuju" Chrysanthemum morifolium. METHODS: All compounds were separated and purified by column chromatography over silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified by spectral methods including 1H-NMR and 13C-NMR. RESULTS: 21 compounds were isolated and identified as octa-cosyl alcohol (1), ß-sitosterol (2), lupeol (3), α-amyrin (4), daucosterol (5), ineupatorolide B (6), syringin (7), chlorogenic acid (8), petasiphenol (9), physcion (10), acacetin (11), eupatilin (12), quercetin (13), diosmetin (14), luteolin (15), apigenin (16), apigenin- 7-O-ß-D-glucopyranoside (17), quercetin-3-O-ß-D-glucopyranoside (18), luteolin-7-O-ß-D-gluco pyranoside (19), apigenin-7-O-ß-D- neospheroside (20), and acacetin-7-O-ß-D-glucoside (21). CONCLUSION: Compounds 1-12, 18 and 20 are isolated from this plant for the first time. Compounds 10, 13, 14, 15 and 16 have shown strong antioxidant activities by DPPH · scavenging activity better than Vit C.
