Assuntos
Aminoácidos/farmacologia , Linfócitos B/imunologia , Ativação Linfocitária , Animais , Anticorpos/farmacologia , Formação de Anticorpos , Linfócitos B/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Focalização Isoelétrica , Cinética , Lipídeo A/imunologia , Ativação Linfocitária/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos C3H , Peso Molecular , Baço/citologia , UltrafiltraçãoRESUMO
The comparative and combined effects of sucrose, maltose, and lactose as factors on the plaque-forming potential of Streptococcus mutans were assessed. With increasing additions of maltose to sucrose-supplemented medium there was decreasing plaque formation. Lactose additions slightly increased plaque formation, but when combined with maltose they significantly enhanced the maltose inhibition of plaque formation.
Assuntos
Placa Dentária/metabolismo , Dissacarídeos/farmacologia , Streptococcus mutans/efeitos dos fármacos , Placa Dentária/microbiologia , Lactose/metabolismo , Lactose/farmacologia , Maltose/metabolismo , Maltose/farmacologia , Streptococcus mutans/metabolismo , Sacarose/metabolismo , Sacarose/farmacologiaRESUMO
The purpose of this study was to examine amylase activity in the parotid saliva of monkeys fed a high sugar diet supplemented for 3-day periods with either sucrose or xylitol. All monkeys fed xylitol displayed a significant elevation of amylase activity and total protein content in their parotid saliva. The specific activity of amylase (units/mg protein) did not differ between the two diets. The possible importance of this observation to the purported cariostatic action of xylitol is discussed.
Assuntos
Amilases/metabolismo , Glândula Parótida/fisiologia , Saliva/metabolismo , Proteínas e Peptídeos Salivares/metabolismo , Xilitol/farmacologia , alfa-Amilases/metabolismo , Animais , Cariostáticos , Carboidratos da Dieta , Haplorrinos , Macaca mulatta , Glândula Parótida/efeitos dos fármacos , Sacarose/farmacologiaRESUMO
A method of polyacrylamide gel electrophoresis for examining histidine-rich-polypeptides in human saliva is described. Comparison is made to several commonly used electrophoretic techniques. The described method allows for the resolution of seven histidine-rich-polypeptide fractions and is convenient and quite reproducible.
Assuntos
Eletroforese em Gel de Poliacrilamida/métodos , Histidina/isolamento & purificação , Peptídeos/isolamento & purificação , Proteínas e Peptídeos Salivares/isolamento & purificação , Resinas de Troca de Cátion , Feminino , Humanos , MasculinoAssuntos
Glândula Parótida/análise , Peptídeos , Proteínas e Peptídeos Salivares , Aminoácidos/análise , Sítios de Ligação , Eletroforese em Gel de Poliacrilamida , Histidina/análise , Humanos , Peso Molecular , Ligação Proteica , Proteínas e Peptídeos Salivares/isolamento & purificação , Reagentes de SulfidrilaRESUMO
Hartley strain guinea pigs were sensitized with 0.5 ml of concentrated cell-free Actinomyces viscosus culture supernatant fluids mixed with Freund complete adjuvant. Fourteen to 16 days later the animals were challenged by intradermal injection with 0.1 ml of the culture supernatant, and the reactions were observed at 4, 8, 16, 24, and 48 h. Peritoneal exudate cells from sensitized animals were used for determination of migration inhibition factor, and guinea pig peripheral blood served as a source of cells for determining the induction of mitogenesis by antigenic material. Skin responses were consistently positive to challenge with the test material, whereas reactions to noninoculated culture medium were negative. Sensitized cells, challenged with antigen, resulted in 60% or greater inhibition of migration of indicator cells in migration inhibition factor experiments. Tests for mitogenesis showed a greater than fourfold increase in isotope uptake when sensitized cells were challenged with test material. The data are consistent with the suggestion that A. viscosus culture supernatants contain substances that induce cell-mediated immune responses in guinea pigs.
Assuntos
Actinomyces/imunologia , Imunidade Celular , Animais , Inibição de Migração Celular , Sistema Livre de Células , Feminino , Cobaias , Ativação Linfocitária , Masculino , Mitógenos/farmacologia , Testes CutâneosRESUMO
Parotid saliva samples from caries-resistant and caries-rampant individuals were electrophoresed on polyacrylamide gel slabs. The parotid saliva of the caries-rampant group showed a significantly higher level of anodemigrating proteins, predominantly isoamylases, and a significantly lower level of cathode-migrating proteins than that of the caries-resistant group in both paraffin-stimulated and sour lemon-stimulated salivary flows.
