RESUMO
A serological survey in Mozambique to detect antibodies to Cowdria ruminantium, the etiologic agent of heartwater, revealed a seroprevalence of 8.1% (n = 332) for goats in the northern province of Tete and of 65.6% (n = 326) for goats in the southern provinces. Translocation of 10 serologically negative goats from Tete to farms in the south resulted in two clinical cases of heartwater that were fatal. In addition, four goats seroconverted within the study period of 5 weeks. One goat showed no symptoms. Two goats died of other causes, whereas the remaining goat went missing after 1 week. Experimental needle infections of goats and sheep were conducted to confirm results and to isolate different strains of C. ruminantium. These data indicate that translocation of goats from the north to the south of Mozambique bears a high risk of C. ruminantium infection, which can cause fatal disease.
Assuntos
Doenças das Cabras/mortalidade , Hidropericárdio/mortalidade , Animais , Anticorpos Antibacterianos/sangue , Anticorpos Antibacterianos/imunologia , Antígenos de Bactérias/imunologia , Proteínas de Bactérias/imunologia , Sequência de Bases , DNA Bacteriano/sangue , Ehrlichia ruminantium/genética , Ehrlichia ruminantium/isolamento & purificação , Ehrlichia ruminantium/fisiologia , Doenças das Cabras/epidemiologia , Doenças das Cabras/microbiologia , Doenças das Cabras/transmissão , Cabras , Hidropericárdio/epidemiologia , Hidropericárdio/microbiologia , Hidropericárdio/transmissão , Proteínas de Membrana/imunologia , Dados de Sequência Molecular , Moçambique/epidemiologia , RNA Bacteriano/análise , RNA Ribossômico 16S/análise , Estudos SoroepidemiológicosRESUMO
Adapting the concept of "bed-side" patient analysis, the Boehringer-Mannheim Reflotron was evaluated for its possible use in veterinary medicine under outdoor-field conditions. Horse blood was analysed with the Cobas Bio analyzer, and indoor and outdoor analyses were also performed with the Reflotron. All values showed close agreement with no significant differences. Good correlation coefficients (r values around 0.9000) were also seen between all methods used. The Reflotron was operated under outdoor-field conditions by using, whenever available, an on-farm electricity source, or a gas operated generator. The data supports the proposal to adapt the Reflotron (or any other proved system) for outdoor-field, patient-side blood analysis, thus providing the veterinary clinician with values for blood quantities, and enabling an improved and more complete diagnosis.
