RESUMO
The porcine PER1 gene was mapped to chromosome 12q1.4-->q1.5 using fluorescence in situ hybridisation. A polymorphic microsatellite marker (S0601) was isolated from a BAC clone shown to contain the PER1 gene. Linkage analysis assigned S0601 distal to ALOX12 on SSC12, providing further evidence for the conservation of synteny between HSA17 and SSC12. RT-PCR analysis demonstrated the expression of PER1 in all 11 tissues tested, consistent with the data from other mammalian species. Part of the PER1 gene was sequenced, homologous to exons 2-14 of the human gene and encoding the N-terminus of porcine PER1. The predicted amino acid sequence of the partial pig PER1 protein shares over 96% identity with its human orthologue.
Assuntos
Mapeamento Cromossômico , Perfilação da Expressão Gênica , Proteínas Nucleares/genética , Mapeamento Físico do Cromossomo , Suínos/genética , Animais , Proteínas de Ciclo Celular , Sequência Conservada/genética , Éxons/genética , Humanos , Hibridização in Situ Fluorescente , Escore Lod , Repetições de Microssatélites/genética , Proteínas Circadianas Period , Polimorfismo Genético/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , SinteniaAssuntos
Antígenos de Neoplasias/genética , Hibridização in Situ Fluorescente , Proteínas Quinases/genética , Mapeamento de Híbridos Radioativos , Suínos/genética , Animais , Antígenos de Neoplasias/química , Cromossomos Humanos Par 12/genética , Clonagem Molecular , Humanos , Camundongos , Dados de Sequência Molecular , Proteínas Quinases/química , Homologia de SequênciaAssuntos
Ligação Genética/genética , Mapeamento Físico do Cromossomo , Receptores de Superfície Celular/genética , Receptores Citoplasmáticos e Nucleares/genética , Suínos/genética , Animais , Feminino , Hibridização in Situ Fluorescente , Escore Lod , Masculino , Repetições de Microssatélites/genética , Polimorfismo Genético/genética , Receptores de MelatoninaRESUMO
The PAX8 gene, a member of the human paired box gene family, was mapped by FISH to chromosome 11 in cattle and goat and to the short arm of chromosome 3 in sheep. The cytogenetic position of PAX8 on BTA 11 and on its homologue OAR 3p lies in the region where the interleukin beta (IL1B) gene has been previously located, (BTA 11q22. 1-->q22.3 and OAR 3p25-->q26 respectively; Lòpez-Corrales et al., 1998). The results indicated that PAX8 as well as interleukin beta and interleukin alpha (IL1B and IL1A) genes detected on the human chromosome segment HSA 2q13-->q21 maintain a similar order and location in these three related species. In addition, the breakpoint in conserved synteny can now be narrowed to a position between the protein C (PROC) and PAX8 genes, which lie in close proximity on HSA 2.
Assuntos
Bovinos/genética , Cromossomos/genética , Proteínas de Ligação a DNA/genética , Cabras/genética , Proteínas Nucleares , Mapeamento Físico do Cromossomo , Ovinos/genética , Transativadores/genética , Animais , Cromossomos Humanos Par 2/genética , Cosmídeos/genética , Sondas de DNA , Evolução Molecular , Humanos , Hibridização in Situ Fluorescente , Interleucina-1/genética , Fator de Transcrição PAX8 , Fatores de Transcrição Box Pareados , Proteína C/genética , Homologia de Sequência do Ácido NucleicoRESUMO
This study provides 53 new fluorescent in situ hybridization cytogenetic assignments for microsatellite markers linked on the swine genetic map. Forty microsatellites are physically assigned for the first time. The chromosomal locations of eight markers were either confirmed or refined, while five loci were assigned to locations different from those given in previous reports. Markers were selected to provide physical anchors based on their presumed proximity to centromeres or telomeres and at approximately 30 cM intervals across the genetic map. The number of physical anchors for pig (SSC) chromosomes 8, 15, and 18 linkage groups was significantly improved. Centromeric regions were localized to areas less than 10 cM for SSC 1, 2, 3, 6, 7, 8, and 9. Although the recombination rate was generally higher across small biarmed chromosomes and lowest for large acrocentric chromosomes, two regions with particularly low (1q2.1-->q2.9 and 13q2.3-->q4.1) and three regions with extremely high (5p1.5-->p1.2, 6p1.4-->p1.3, and 12p1.5-->p1.4) rates of recombination were detected. These assignments represent an overall 10% increase in the number of physically assigned markers in Sus scrofa and more than a 20% increase in the number of Type II loci assigned to the pig cytogenetic map.
Assuntos
Repetições de Microssatélites , Suínos/genética , Animais , Bandeamento Cromossômico , Mapeamento Cromossômico , Cromossomos Artificiais de Levedura/genética , Citogenética , Ligação Genética , Hibridização in Situ Fluorescente , Mapeamento Físico do CromossomoRESUMO
Seasonal infertility in sows is a problem in the pig industry characterized by delayed onset of puberty in summer and decreased farrowing rate resulting from silent oestrus and aborted pregnancy. Summer infertility is thought to be influenced by heat, sunburn and stress. However, the strongest contributory factor is photoperiod. The difference in seasonality between wild boar and commercial pig breeds suggests that there may be a genetic component to this trait. The maps and associated molecular tools emerging from the pig genome project have created opportunities to examine the genetic component of seasonal infertility. We are identifying and mapping genes that are likely to be involved in biological clock mechanisms and the melatonin pathways as candidate seasonality genes.
Assuntos
Mapeamento Cromossômico , Genoma , Infertilidade/veterinária , Suínos/genética , Aborto Animal/genética , Animais , Feminino , Infertilidade/genética , Melatonina/genética , Fotoperíodo , Gravidez , Estações do AnoRESUMO
The cytogenetic locations of the genes for protein C (PROC), transition protein 1 (TNP1), intestinal alkaline phosphatase (ALPI), engrailed (EN1), and human protointerleukin beta (IL1B) have been compared between cattle (Bos taurus, BTA) and sheep (Ovis aries, OAR). Bovine YAC and cosmid clones were used as FISH probes to determine the order (centromere to telomere) of four of these genes on OAR 2q, as well as the location of IL1B on OAR 3p. In cattle, IL1B and EN1 were assigned to BTA 11 and BTA 2, respectively. Alignment of the ovine, bovine, and human physical maps based on these data shows that segments of conserved synteny and chromosomal rearrangements detected between cattle and human are also found in sheep, where the order in cattle is conserved.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Proteínas de Transporte/genética , Proteínas Cromossômicas não Histona/genética , Proteínas de Homeodomínio/genética , Interleucina-1/genética , Proteínas do Tecido Nervoso , Proteína C/genética , Fatores de Transcrição , Proteínas Supressoras de Tumor , Animais , Bovinos , Bandeamento Cromossômico , Mapeamento Cromossômico , Rearranjo Gênico , Genoma Humano , Humanos , Hibridização in Situ Fluorescente , Ovinos , Domínios de Homologia de src/genéticaRESUMO
Myostatin (GDF-8) is a member of the transforming growth factor-beta superfamily and plays a role in muscle growth and development. Mice having targeted disruption of this gene display marked increases in muscle mass, a phenotype similar to the muscular hypertrophy (mh) in several cattle breeds. Physical mapping data developed from YAC clones indicate the bovine myostatin gene lies close to the centromere of bovine Chromosome (Chr) 2 (BTA2) at 2q11, indistinguishable from the cytogenetic location of the mh locus. In addition, a polymorphism in the second intron of the gene was used to show that myostatin maps within the interval previously shown to contain mh. These data suggest myostatin may be the gene causing muscular hypertrophy in cattle.
Assuntos
Genes , Fator de Crescimento Transformador beta/genética , Animais , Bovinos , Mapeamento Cromossômico , Cromossomos Artificiais de Levedura , Hipertrofia , Hibridização in Situ Fluorescente , Músculos/patologia , Miostatina , Fenótipo , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de RestriçãoRESUMO
The "double-muscling" (mh) locus has been localized to an interval between the centromere and the microsatellite marker TGLA44 on bovine Chromosome (Chr) 2 (BTA2). We identified segments of conserved synteny that correspond to this region of BTA2 by assigning large genomic clones containing bovine homologs of seven genes from the long arm of human Chr 2 (HSA2q). Polymorphic markers developed from these clones integrated the physical and linkage maps of BTA2 from 2q12 to 2q44 and extended genetic coverage towards the centromere. This comparative analysis suggests the mh locus resides on HSA2q near both the protein C and collagen type III alpha-1 genes. Overall, our data reveal a complex rearrangement of gene order between BTA2q12-44 and HSA2q14-37 that underscores the need to establish boundaries of conserved synteny when applying comparative mapping information to identify genes or traits of interest.
Assuntos
Mapeamento Cromossômico , Cromossomos Humanos Par 2/genética , Genes , Repetições de Microssatélites , Proteínas Musculares/genética , Animais , Bovinos , Centrômero , Colágeno/genética , Cosmídeos , DNA/isolamento & purificação , Rearranjo Gênico , Humanos , Hibridização in Situ Fluorescente , Proteína C/genética , Homologia de SequênciaRESUMO
We report a bovine linkage map constructed with 1236 polymorphic DNA markers and 14 erythrocyte antigens and serum proteins. The 2990-cM map consists of a sex-specific, X chromosome linkage group and 29 sex-averaged, autosomal linkage groups with an average interval size of 2.5 cM. The map contains 627 new markers and 623 previously linked markers, providing a basis for integrating the four published bovine maps. Orientation and chromosomal assignment of all the linkage groups, except BTA20 and BTA22, was provided by 88 markers that were assigned previously to chromosomes. This map provides sufficient marker density for genomic scans of populations segregating quantitative trait loci (QTL) and subsequent implementation of marker-assisted selection (MAS) mating schemes.
Assuntos
Mapeamento Cromossômico , Ligação Genética , Animais , Proteínas Sanguíneas , Bovinos , Cromossomos/genética , DNA/análise , Eritrócitos , Marcadores Genéticos , Biblioteca Genômica , Repetições de Microssatélites , Dados de Sequência Molecular , Polimorfismo Genético , Cromossomo X/genética , Cromossomo Y/genéticaRESUMO
Genotypic data for 56 microsatellites (ms) generated from maternal full sib families nested within paternal half sib pedigrees were used to construct a linkage map of the bovine X Chromosome (Chr) (BTX) that spans 150 cM (ave. interval 2.7 cM). The linkage map contains 36 previously unlinked ms; seven generated from a BTXp library. Genotypic data from these 36 ms was merged into an existing linkage map to more than double the number of informative BTX markers. A male specific linkage map of the pseudoautosomal region was also constructed from five ms at the distal end of BTXq. Four informative probes physically assigned by fluorescence in situ hybridization defined the extent of coverage, confirmed the position of the pseudoautosomal region on the q-arm, and identified a 4.1-cM marker interval containing the centromere of BTX.
