RESUMO
This review deals with the development of new methods for studies of blood cells and plasma, based on the use of special dye molecules, the so-called fluorescent probes. These probes can also be used for clinical diagnosis. Probes and new methods on the basis of these probes were created for measurements of plasma and serum lipoproteins, serum albumin binding centers, blood leukocyte intracellular lipoproteins, allergens.
Assuntos
Células Sanguíneas/química , Análise Química do Sangue , Corantes Fluorescentes/química , Plasma/química , Células Sanguíneas/metabolismo , Análise Química do Sangue/instrumentação , Análise Química do Sangue/métodos , Corantes Fluorescentes/metabolismo , Humanos , Estrutura Molecular , Plasma/metabolismo , Albumina Sérica/química , Albumina Sérica/metabolismoRESUMO
The negative surface charge of low-density lipoproteins increased during their oxidative modification induced by autooxidation at 37 degrees C. The degree of changes depended on the time of autooxidation: the surface charge remained practically unchanged after short-term oxidation (6-h incubation), but then progressively increased and after 24-h oxidation it 2-fold surpassed the initial level. Long-term incubation of low-density lipoproteins in the presence of EDTA inhibiting lipid peroxidation did not change their surface charge. These changes probably contribute to atherogenic activity of oxidized low-density lipoproteins. The degree of oxidative modification of low-density lipoproteins was precisely estimated using fluorescence probes.
Assuntos
Lipoproteínas LDL/química , Lipoproteínas LDL/metabolismo , Ânions , Quelantes/farmacologia , Ácido Edético/farmacologia , Eletroforese em Gel de Ágar , Corantes Fluorescentes/química , Humanos , Técnicas In Vitro , Malondialdeído/metabolismo , Concentração Osmolar , Oxirredução , Conformação Proteica , Propriedades de SuperfícieRESUMO
We evaluated antioxidant state of 62 patients with coronary heart disease and 47 patients with obliterating atherosclerosis of lower extremities by peroxide-depend chemiluminescence and inhibition of azo-initiated chemiluminescence and hydrogen peroxide-hemoglobin-luminol chemiluminescence system. The flash amplitude of peroxide-dependent chemiluminescence in the plasma from patients was 32% below the control. Antioxidant activity of the plasma from patients was higher than in healthy individuals by 33 and 27% depending on the type of free radical-generating systems. The increase in antioxidant activity was most pronounced in patients with combined pathology: coronary heart disease complicated by obliterating atherosclerosis. These results explain the decrease in peroxide-dependent chemiluminescence of the plasma and whole blood in patients with atherosclerosis compared to that in healthy individuals.
Assuntos
Antioxidantes/metabolismo , Antioxidantes/farmacologia , Arteriosclerose/sangue , Arteriosclerose/metabolismo , Medições Luminescentes , Estudos de Casos e Controles , Progressão da Doença , Radicais Livres , Humanos , Peróxido de Hidrogênio/farmacologiaRESUMO
In vitro experiments showed that copper-oxidized low-density lipoproteins activate factors of the prothrombin complex in the whole blood and inhibit fibrin generation in both blood and plasma. Moreover, oxidized low-density lipoproteins inhibit fibrinolysis and impair the structure of fibrin clot, which results in hypercoagulation.
Assuntos
Coagulação Sanguínea/efeitos dos fármacos , Fibrinólise/efeitos dos fármacos , Lipoproteínas LDL/farmacologia , Cobre/metabolismo , Humanos , Lipoproteínas LDL/sangue , Oxirredução , Tempo de Protrombina , Tromboelastografia , Tempo de TrombinaRESUMO
A new fluorescent test developed at the Institute of Physicochemical Medicine (Ministry of Health of the Russian Federation) allows to determine not only blood concentration of albumin, but also to evaluate the state of its molecule. The test is feasible and enables express-analysis of the plasma and serum without fractionation and other preliminary procedures. This test reveals abnormalities in albumin molecule caused by toxic metabolites or conformational changes. Many diseases are accompanied by conformational changes in albumin, while its concentration often remains unchanged. Changes in albumin conformation can serve a diagnostic and prognostic criterion in some pathologies.