RESUMO
Evasion of the immune system by viruses is a well-studied field. It remains a challenge to understand how these viral tactics affect pathogenesis and the viral lifecycle. At the same time, the study of viral proteins involved in immune evasion has helped us to better understand a number of cellular processes at the molecular level. Here we review recent data on different viral tactics for immune evasion and highlight what these viral interventions might teach us about cell biology.
Assuntos
Viroses/imunologia , Viroses/virologia , Animais , Regulação para Baixo/genética , Regulação para Baixo/imunologia , Genes MHC Classe I/imunologia , Genes Virais , Humanos , Imunidade Ativa/genética , Imunidade Celular/genética , Regulação para Cima/genética , Regulação para Cima/imunologia , Proteínas Virais/genética , Proteínas Estruturais Virais/genética , Viroses/genéticaRESUMO
A colorimetric procedure was developed for the quantification of gentamicin. The method was based on the ninhydrin reaction with primary and secondary amines present in the gentamicin. This reaction produces a purple colour. The effects of several factors including pH, ninhydrin concentration and reaction time were investigated to optimize the assay method. Using the assay protocol, the absorption of the gentamicin-ninhydrin mixtures at 400 nm had a linear relationship with the gentamicin concentration ranging from 30 to 120 microg/ml. The colorimetric gentamicin assay reported herein is of great practical value because it is reproducible, sensitive, simple and extremely inexpensive.
