[Isolation of genetically modified potato plant containing the gene of defensive peptide from Amaranthus]. / Poluchenie geneticheski modifitsirovannykh rastenii kartofelia, nesushchikh gen zashchitnogo peptida amaranta.

The plants of potato (Solanum tuberosum L., var. Desire) have been transformed with a pH22Kneo vector carrying the gene ac2, encoding the fungicidal peptide (defensin) from the seed of amaranth (Amaranthus caudatus L.). The transformation involved co-cultivation of potato stem explants (excised from aseptically grown plants) and Agrobacterium tumefaciens on solid MS medium. Factors affecting in vitro regeneration of the explants and the transformation efficiency were optimized. Regenerated potato plants harboring the amaranth defensin gene were selected by two traits, growth and ability to form roots on kanamycin-supplemented MS medium. The transgenic state was confirmed PCR analysis of ac2 in tissues of the kanamycin-resistant plants. The transgenic organisms thus obtained differed from the original ambiol-treated plants in growth patterns and proton translocation across the plasma membrane of the tuber cells.