Assuntos
Hiperlipidemias/sangue , Lipoproteínas/sangue , Adulto , Colesterol/análise , Colesterol/sangue , Gorduras na Dieta/farmacologia , Jejum , Humanos , Lipoproteínas/análise , Masculino , Fosfolipídeos/análise , Fosfolipídeos/sangue , Triglicerídeos/análise , Triglicerídeos/sangue , UltracentrifugaçãoAssuntos
Gorduras na Dieta , Mucosa Intestinal/metabolismo , Lipoproteínas/metabolismo , Linfa , Animais , Antígenos , Autoanálise , Proteínas Sanguíneas/metabolismo , Peso Corporal , Colesterol/metabolismo , Computadores , Deficiências Nutricionais/metabolismo , Soros Imunes , Imunoeletroforese , Intestinos/efeitos dos fármacos , Lipoproteínas/análise , Lipoproteínas/sangue , Masculino , Ácido Orótico/farmacologia , Fosfolipídeos/sangue , Fosfolipídeos/metabolismo , Proteínas/metabolismo , Ratos , Triglicerídeos/metabolismo , UltracentrifugaçãoRESUMO
A procedure is described for the separation of plasma S(f) > 400 and S(f) 20-400 lipoproteins each into three fractions. Serum samples are overlayered with a sodium chloride density gradient in a preparative ultracentrifuge tube and thin layers are removed at the top of the tube after successive centrifugations at different speeds in a swinging bucket rotor. The procedure was evaluated by electron microscopy of the S(f) > 400 lipoprotein fractions and schlieren analysis of the S(f) 20-400 lipoprotein fractions. Protein content of each fraction was measured by elemental N, C, H, and lipid-P analysis. Protein coverage was calculated for all fractions on the assumption that there is a surface layer 20 A thick. For the entire S(f) > 400 lipoprotein spectrum and for a part of the S(f) 20-400 lipoprotein distribution the proportion of surface covered by protein was constant (approximately 20% coverage). Therefore, for these portions of the lipoprotein spectrum, the increase in surface: volume ratio as particle size decreases is approximately compensated for by an increase in the weight percentage of protein.
