RESUMO
OBJECTIVE: To establish the HPLC fingerprint analysis method for the quality control of acetoacetate extraction of Polygonum orientale. METHODS: Agilent C18 (4.6 mm x 250 mm, 5 microm); DAD detector; Mobile phase: methyl alcohol-water gradient elution at a flow rate of 1.0 mL/min, and at a column temperature of 30 degrees C. RESULTS: 26 peaks were selected as the common fingerprint peaks. The relative standard deviations for relative retention values were less than 3% in the precision and repeated test. CONCLUSION: The repetition, stability and precision of the method is good and it can be used to control the quality of Polygonum orientale.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/química , Plantas Medicinais/química , Polygonum/química , Acetoacetatos/química , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Medicamentos de Ervas Chinesas/isolamento & purificação , Plantas Medicinais/crescimento & desenvolvimento , Polygonum/crescimento & desenvolvimento , Controle de Qualidade , Reprodutibilidade dos TestesRESUMO
OBJECTIVE: To compare HPLC fingerprint of flavonoids in fruits of Polygonnm orientale from different regions. METHODS: Diamonsil C18 (250 mm x 4.6 mm, 5 microm) had been used, the mobile phase consisted of methanol and 0.2% phosphoric acid as fradient eluent. The flow rate was 1 ml/min, and the detection wavelength was 310 nm. RESULTS: The finerprint of flaxonoids in fruits of Poligonum orientale with 11 common peaks was eatablished. The realative retention time and the range of relative area of the common peaks were determined. CONCLUSION: The established fingerprint can be used for the identification and quality control of flavonoids of Poligonum orientale from different regions.
