[Benz(a)anthracene and 2,3,7,8-tetrachloro dibenzo(p)dioxin modulate mitogen-stimulated proliferation of lymphocytes]. / Benz(a)antratsen i 2,3,7,8-tetrakhlordibenzo(p)dioksin modiliruiut mitogenstimulirovannuiu proliferatsiiu limfotsitov.

The influence of benzo(a)anthracene (BA) and 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) on functional properties of peripheral blood mononuclear cells (PBC) has been investigated. Incubation of mitogen-stimulated cells in the presence of xenobiotics induced high activity of benzo(a) pyrene-hydroxylase (BH) and suppressed lymphocyte blast transformation. Preincubation of unstimulated PBC with BA and TCDD caused insignificant increase of BH activity. The results show modulated effect of xenobiotics on functional properties of PBC.

[Benzopyrene hydroxylase induction and the functioning of the immunocompetent cells in human peripheral blood]. / Induktsiia benzpirengidroksilazy i funktsional'noe sostoianie immunokompetentnykh kletok perifericheskoi krovi cheloveka.

Xenobiotics--inducers of benzo(a)pyrene hydroxylase (BPH)--exert different effects on mitogen-stimulated and mitogen-unstimulated human peripheral blood mononuclear cells (PBC). In mitogen-stimulated culture xenobiotics highly increase BPH activity and suppress cell blast transformation. The incubation of the unstimulated PBC in the presence of xenobiotics increases insignificantly BPH activity, intensifies T-cell differentiation and concanavalin A-induced proliferation. The BPH activity is mainly associated with the PBC adhered to plastic Petri dishes. However, the control and induced levels of BPH activity depend on the interaction between adhered and nonadhered cells.