Plasma serotonin in autism.

Serotonin is necessary for normal fetal brain development. Administration of serotonin inhibitors to pregnant rats results in offspring with abnormal behaviors, brain morphology, and serotonin receptor numbers. Low maternal plasma serotonin may contribute to abnormal brain development in autism. In this study, plasma serotonin levels in autism mothers and control mothers of typically developing children were compared, and plasma serotonin levels in children with autism (n = 17) and their family members were measured. Plasma serotonin levels in autism mothers were significantly lower than in mothers of normal children (P = 0.002). Plasma serotonin levels correlated between autism mothers and their children, but differed between autistic children and their fathers (P = 0.028) and siblings (P = 0.063). Low maternal plasma serotonin may be a risk factor for autism through effects on fetal brain development.

HL-60 cell growth-conditioned medium is an effective inducer of myeloperoxidase expression in K-562 human leukemia cells.

K-562 cells were cultured in HL-60 cell growth-conditioned medium (GCM) for up to 96h. Myeloperoxidase (MPO) mRNA was transiently detected by reverse transcription-polymerase chain reaction (RT-PCR) techniques at 12, 24, and 48h. The de novo expression of MPO protein was subsequently detectable by intracellular flow cytometry at 24, 48, 72 and 96h. Immunogold staining and cytochemical analysis demonstrated granularly-sequestered MPO in approximately 40% of HL-60 GCM-cultured cells after 48h of culture. The sequential detection of MPO mRNA and MPO biosynthesis is considered an indicator of serial maturation evocative of myeloblastic cells, and suggest that K-562 cells maintain the ability to differentiate along this lineage.