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Vaccine ; 32(3): 401-8, 2014 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-24252697

RESUMO

The fungus of Ganoderma is a basidiomycete that possesses a variety of pharmacological effects and has been used in traditional Asian medicine for centuries. Ganoderma formosanum is a native Ganoderma species isolated in Taiwan, and we have previously demonstrated that PS-F2, a polysaccharide fraction purified from the submerged culture broth of G. formosanum, exhibits immunostimulatory properties in macrophages. In this study, we further characterized the adjuvant functions of PS-F2. In vitro, PS-F2 stimulated dendritic cells (DCs) to produce proinflammatory cytokines, including TNF-α, interleukin (IL)-6, and IL-12/IL-23 p40. PS-F2 also stimulated DCs to express the maturation markers CD40, CD80, CD86, and MHC class II. In a murine splenocyte culture, PS-F2 treatment resulted in elevated expression of T-bet and interferon (IFN)-γ in T lymphocytes. When used as an adjuvant in vivo with the ovalbumin (OVA) antigen, PS-F2 stimulated OVA-specific antibody production and primed IFN-γ production in OVA-specific T lymphocytes. PS-F2-adjuvated immunization also induced OVA-specific CTLs, which protected mice from a challenge with tumor cells expressing OVA. Collectively, our data show that PS-F2 functions as an adjuvant capable of inducing a Th1-polarized adaptive immune response, which would be useful in vaccines against viruses and tumors.


Assuntos
Adjuvantes Imunológicos/farmacologia , Ganoderma/química , Polissacarídeos/farmacologia , Linfócitos T Citotóxicos/imunologia , Células Th1/imunologia , Adjuvantes Imunológicos/isolamento & purificação , Animais , Células Dendríticas/efeitos dos fármacos , Células Dendríticas/imunologia , Interferon gama/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Ovalbumina/administração & dosagem , Ovalbumina/imunologia , Polissacarídeos/isolamento & purificação , Linfócitos T Citotóxicos/efeitos dos fármacos , Taiwan , Células Th1/efeitos dos fármacos , Vacinas/administração & dosagem , Vacinas/imunologia
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