Fas-FasL expression and myocardial cell apoptosis in patients with viral myocarditis.

The aim of the current study was to investigate Fas and FasL expression and myocardial cell apoptosis in viral myocarditis patients. Human heart specimens were selected from patients who were autopsied between February 2012 and February 2015; of these, 25 patients were diagnosed with viral myocarditis. Another 15 cases with no diagnosis of myocarditis were selected for the control group. All tissue specimens were divided into two parts, one for reverse transcription-polymerase chain reaction analysis and the other for immunohistochemical and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analyses. In situ detection of apoptosis was performed by the TUNEL method, which revealed that myocardial cells from the viral myocarditis group exhibited significant apoptosis, whereas no apoptotic cells were observed in the control group. The number of cells staining positive for Fas and FasL protein in the viral myocarditis group was significantly higher than that in the control group (P < 0.05). There was also a correlation between Fas and FasL protein expression levels and scores (r = 0.92, P < 0.05). The mRNA expression of Fas and FasL was significantly higher in the viral myocarditis group than in the control group (P < 0.05). In conclusion, the Fas-FasL system may be involved in the pathogenesis of viral myocarditis. Furthermore, cytotoxic T lymphocytes may mediate cardiac muscle cells apoptosis via Fas-FasL signaling, and thus participate in the pathogenesis of viral myocarditis.

Urban transportation energy and carbon dioxide emission reduction strategies.

Sustainability is an urban development priority. Thus, energy and carbon dioxide emission reduction is becoming more significant in the sustainability of urban transportation systems. However, urban transportation systems are complex and involve social, economic, and environmental aspects. We present solutions for a sustainable urban transportation system by establishing a simplified system dynamics model with a timeframe of 30 years (from 1995 to 2025) to simulate the effects of urban transportation management policies and to explore their potential in reducing vehicular fuel consumption and mitigating CO2 emissions. Kaohsiung City was selected as a case study because it is the second largest metropolis in Taiwan and is an important industrial center. Three policies are examined in the study including fuel tax, motorcycle parking management, and free bus service. Simulation results indicate that both the fuel tax and motorcycle parking management policies are suggested as potentially the most effective methods for restraining the growth of the number of private vehicles, the amount of fuel consumption, and CO2 emissions. We also conducted a synthetic policy consisting of all policies which outperforms the three individual policies. The conclusions of this study can assist urban transport planners in designing appropriate urban transport management strategies and can assist transport operation agencies in creating operational strategies to reduce their energy consumption and CO2 emissions. The proposed approach should be generalized in other cities to develop an appropriate model to understand the various effects of policies on energy and CO2 emissions.