RESUMO
Long non-coding RNAs (lncRNAs) are crucial modulators of post-transcriptional gene expression regulation, cell fate determination, and disease development. However, lncRNA functions during short-term heat stress in adult worker bees are poorly understood. Here, we performed deep sequencing and bioinformatic analyses of honeybee lncRNAs. RNA interference was performed by using siRNA targeting the most highly expressed lncRNA. The silencing effect on lncRNA and the relative expression levels of seven heat shock protein (HSP) genes, were subsequently examined. Overall, 7,842 lncRNAs and 115 differentially expressed lncRNAs (DELs) were identified in adult worker bees following heat stress exposure. Structural analysis revealed that the overall expression abundance, length of transcripts, exon number, and open reading frames of lncRNAs were lower than those of mRNAs. GO analysis revealed that the target genes were mainly involved in "metabolism," "protein folding," "response to stress," and "signal transduction" pathways. KEGG analysis indicated that the "protein processing in endoplasmic reticulum" and "longevity regulating pathway-multiple species" pathways were most enriched. Quantitative real-time polymerase chain reaction (qRT-PCR) detection of the selected DELs confirmed the reliability of the sequencing data. Moreover, the siRNA experiment indicated that feeding siRNA yielded a silencing efficiency of 77.51% for lncRNA MSTRG.9645.5. Upon silencing this lncRNA, the expression levels of three HSP genes were significantly downregulated (p < 0.05), whereas those of three other HSP genes were significantly upregulated (p < 0.05). Our results provide a new perspective for understanding the regulatory mechanisms of lncRNAs in adult worker bees under short-term heat stress.
Assuntos
Resposta ao Choque Térmico , RNA Longo não Codificante , Animais , Abelhas/genética , Abelhas/fisiologia , RNA Longo não Codificante/genética , Resposta ao Choque Térmico/genética , Proteínas de Choque Térmico/genética , Proteínas de Choque Térmico/metabolismo , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Interferência de RNA , Sequenciamento de Nucleotídeos em Larga Escala , Biologia Computacional/métodosRESUMO
OBJECTIVE: To estimate the exposure characteristic of six emerging brominated flame retardant for Beijing residents by dietary intake. METHODS: 2,3,5,6-tetrabromo-p-xylene (pTBX), pentabromotoluene (PBT), 1, 2, 3, 4, 5-pentabromo-6- ethylbenzene (PBEB), hexahromobenzene (HBB), hexachlorocyclopentadienyl- dibromocyclooctane (DBHCTD) and 1,2-bis(2 ,4 ,6-tribromo phenoxy) ethane (BTBPE) were detected by atmospheric pressure gas chromatography tandem mass spectrometry (APGC-MS/MS) in total dietary samples from Beijing. Dietary intake assessments of human exposure were carried out according to results of determination. RESULTS: PBT, PBEB, HBB and BTBPE were occurred with concentration between 1.2-29.4 pg/g wet weight. The detection rates of HBB and BTBPE in animal--origin samples were higher than those in plant-origin samples, and there were significant differences (P < 0.05). The exposure level of Beijing residents to six emerging brominated flame retardants were 296.8 pg/(kg-d). CONCLUSION: The exposure level of emerging brominated flame retardants by dietary intake in Beijing is relatively low, and meat is the main source of BTBPE dietary intake.
