RESUMO
OBJECTIVES: To investigate the prevalence and influencing factors of stress urinary incontinence (SUI) within 6-8 weeks postpartum in Jiangsu Province. MATERIAL AND METHODS: We designed a multi-center cross-sectional study involving seven hospitals in Jiangsu province, and enrolled women who underwent postpartum examination at 6-8 weeks in these hospitals between July 2019 and June 2021. According to the presence or absence of SUI, the enrolled patients were divided into two groups: the SUI group and the non-SUI group, respectively. We assessed the general health status, noted the details of delivery, and checked the pelvic floor electromyographic parameters of the postpartum women in both groups. RESULTS: Among 6,302 cases of postpartum women in Jiangsu province, there were 1,579 cases of SUI, with a prevalence of 25.06%. The prevalence of SUI increased significantly with age, BMI, increasing parity, coexisting constipation, organ prolapse, and diastasis recti abdominis. Compared to the non-SUI group, the SUI group had a lower mean value of the pre-baseline rest phase, shorter rise and fall times of fast muscle contractions, and a lower mean value of the endurance contraction phase. Multiple regression analysis revealed associations with weight (especially overweight and obesity), coexisting organ prolapse, constipation, parity, gestational week of delivery, mode of delivery, and mean value of endurance contraction phase. CONCLUSIONS: The prevalence of postpartum stress urinary incontinence in Jiangsu Province was 25.06%, and was linked to being overweight, parity > 2, coexisting organ prolapse, constipation, and a decrease in the mean value of the endurance contraction phase of the electromyograph. In this report, we offer a theoretical basis for the effective prevention of postpartum SUI clinically.
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OBJECTIVE: To predict structure and function of translationally controlled tumor protein (TCTP) from Spirometra mansoni by bioinformatics technology, and to provide a theoretical basis for further study. METHODS: Open reading frame (ORF) of EST sequence from Spirometra mansoni was obtained by ORF finder and was translated into amino acid residue by DNAclub. The structure domain was analyzed by Blast. By the method of online analysis tools: Protparam, InterProScan, protscale, SignalP-3.0, PSORT II, BepiPred, TMHMM, VectorNTI Suite 9 packages and Phyre2, the structure and function of the protein were predicted and analyzed. RESULTS: The results showed that the EST sequence was Sm TCTP with 173 amino acid residues, theoretical molecular weight was 19 872.0 Da. The protein has the closest evolutionary status with Clonorchis sinensis, Schistosoma mansoni, and Schistosoma japonicum. Then it had no signal peptide site and transmembrane domain. Secondary structure of TCTP contained two α -helices and eight ß -strands. CONCLUSIONS: Sm TCTP was a variety of biological functions of protein that may be used as a vaccine candidate molecule and drug target.
Assuntos
Biomarcadores Tumorais/química , Infecções por Cestoides/veterinária , Proteínas de Helminto/química , Spirometra/genética , Spirometra/parasitologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Infecções por Cestoides/parasitologia , Biologia Computacional , Doenças do Cão , Cães , Etiquetas de Sequências Expressas , Proteínas de Helminto/genética , Proteínas de Helminto/metabolismo , Helmintos/química , Helmintos/classificação , Helmintos/genética , Fases de Leitura Aberta , Filogenia , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Spirometra/química , Spirometra/metabolismo , Proteína Tumoral 1 Controlada por TraduçãoRESUMO
Full length sequence of lactate dehydrogenase gene was amplified by PCR from the genomic DNA of Plasmodium vivax Hainan isolate, and named as PvLDH/HN (GenBank No. FJ527750). Sequence analysis showed that the gene had 951 bp, coding 316 aa. Compared with PvLDH/Salvador and PvLDH/Belem, the nucleotide sequence homology of PvLDH/HN was both 99.89%, while the homology of amino acid sequence was 100%. Topology analysis showed that the protein had two transmembrane alpha-helices, which suggested that the protein might be a membrane protein. The major antigen epitope regions (82-95aa) was presented on the protein surface, and formed the specific substrate binding loop, which revealed that it might be an ideal target site for drug action and immunodiagnosis.
Assuntos
L-Lactato Desidrogenase/genética , Plasmodium vivax/enzimologia , Plasmodium vivax/genética , Proteínas de Protozoários/genética , Sequência de Aminoácidos , Clonagem Molecular , DNA de Protozoário/genética , Dados de Sequência Molecular , Análise de Sequência de DNA , Homologia de Sequência de AminoácidosRESUMO
The full-length pBluescript II SK cDNA library of adult Spirometra erinaceieuropaei was constructed by using the SMART method. Data showed that 95.5% of the library was recombinant and the titer of the library was 1.06 x 10(6). The average insert size of the library was about 1.4 kb. Forty-eight randomly selected clones were sequenced. A set of 36 effective expressed sequence tags (ESTs) with the average size of 674 bp was obtained after excluding clones shorter than 450 bp. The unigenes occupied 58.3% of the 36 ESTs. The rate of full-length cDNAs were 57.7% (15/26). The high-quality of full-length cDNA library could be used for large scale EST sequencing.
