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1.
Cancer Cell Int ; 22(1): 101, 2022 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-35241075

RESUMO

BACKGROUND: Emerging evidence suggests that LMNB1 is involved in the development of multiple cancer types. However, there is no study reporting the potential role of LMNB1 in a systematic pan-cancer manner. METHODS: The gene expression level and potential oncogenic roles of LMNB1 in The Cancer Genome Atlas (TCGA) database were analyzed with Tumor Immune Estimation Resource version 2 (TIMER2.0), Gene Expression Profiling Interactive Analysis version 2 (GEPIA2), UALCAN and Sangerbox tools. Pathway enrichment analysis was carried out to explore the possible mechanism of LMNB1 on tumorigenesis and tumor progression. The therapeutic effects of LMNB1 knockdown combined with PARP inhibition on human cancers were further investigated in vitro. RESULTS: LMNB1 upregulation is generally observed in the tumor tissues of most TCGA cancer types, and is verified in kidney renal clear cell carcinoma using clinical specimens of our institute. High level of LMNB1 expression usually predicts poor overall survival and disease free survival for patients with tumors. Mechanically, LMNB1 level is positively correlated with CD4+ Th2 cell infiltration and DNA homologous recombination repair gene expression. In vitro experiments reveal that targeting LMNB1 has a synergistic effect on prostate cancer with PARP inhibitor treatment. CONCLUSIONS: LMNB1 is a biomarker of CD4+ Th2 cell infiltration and DNA homologous recombination repair in human cancers. Blockage of LMNB1 combined with PARP inhibitor treatment could be a promising therapeutic strategy for patients with cancers.

2.
BMC Med Imaging ; 22(1): 3, 2022 01 04.
Artigo em Inglês | MEDLINE | ID: mdl-34983423

RESUMO

BACKGROUND: To investigate the application value of the treatment of breast cancer bone metastases with radioactive seed 125I implantation under CT-guidance. METHODS: A total of 90 patients with breast cancer admitted to our hospital from January 2017 to January 2018 were selected as the research objects and were divided into control group and experimental group according to random grouping, with 45 cases in each group. Conventional treatment was used in the control group, while the treatment of radioactive seed 125I implantation under CT-guidance was used in the experimental group. The clinical efficacy, pain intensity and levels of carcinoembryonic antigen (CEA), carcinoembryonic antigen 153 (CA153), carbohydrate antigen (CA125) in the two groups were compared. RESULTS: As for the pain intensity, it was evidently lower in the experimental group after treatment than that in the control group (P < 0.05); as for the total effective rate, it was obviously higher in the experimental group after treatment than that in the control group (P < 0.05); as for the levels of CEA, CA153 and CA125, the data in the experimental group after treatment were much lower than the control group (P < 0.05). CONCLUSION: Radioactive seed 125I implantation under CT-guidance can effectively improve the effect of the treatment of breast cancer bone metastases. It has curative efficacy and it is worth promoting and using.


Assuntos
Neoplasias Ósseas/radioterapia , Neoplasias Ósseas/secundário , Braquiterapia/métodos , Neoplasias da Mama/patologia , Radioisótopos do Iodo , Tomografia Computadorizada por Raios X , Adulto , Idoso , Antígenos de Neoplasias/sangue , Neoplasias Ósseas/sangue , Neoplasias Ósseas/diagnóstico por imagem , Neoplasias da Mama/sangue , Antígeno Ca-125/sangue , Antígeno Carcinoembrionário/sangue , Humanos , Proteínas de Membrana/sangue , Pessoa de Meia-Idade , Medição da Dor
3.
Cancer Lett ; 522: 1-13, 2021 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-34520818

RESUMO

The metastatic dissemination and underlying mechanisms of clear cell renal cell carcinoma (ccRCC) remain insufficiently understood. In this study, we identified the essential role of KLF2 in suppressing the metastasis of ccRCC. Downregulation of KLF2 detected by immunohistochemistry in primary metastatic ccRCC was remarkably related to poor clinical outcomes. Overexpression of KLF2 in vitro inhibited growth, migration and invasion of RCC cells. Analysis of clinical specimens revealed that there is a close correlation between KLF2 and GPX4 in ccRCC. Mechanistically, KLF2 deficiency is sufficient to inhibit ferroptosis on account of the impairment of transcriptional repression of GPX4 and thus promotes the migration and invasion of RCC cells. Reverting KLF2 expression in vivo decreased pulmonary metastatic lesions and prolonged life span of mice, whereas GPX4 overexpression reversed these properties. Overall, our results established a novel critical pathway that drives human ccRCC invasion and metastasis, which could be a promising target regarding to the therapies of advanced ccRCC in the clinic.


Assuntos
Carcinoma de Células Renais/genética , Ferroptose/genética , Fatores de Transcrição Kruppel-Like/genética , Fosfolipídeo Hidroperóxido Glutationa Peroxidase/genética , Idoso , Animais , Carcinoma de Células Renais/patologia , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Transição Epitelial-Mesenquimal/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Masculino , Camundongos , Pessoa de Meia-Idade , Invasividade Neoplásica/genética , Invasividade Neoplásica/patologia , Metástase Neoplásica , Prognóstico
5.
Int J Mol Med ; 40(6): 1907-1913, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29039458

RESUMO

Eco1/Eso1 protein plays an important role in chromosome segregation, DNA repair and gene regulation. Eco1 mutation induces Roberts syndrome clinically and rDNA transcription disorders in vivo. In this study, we examined the role of Eso1 protein binding to polymerase 5 (Pol5) and the acetylation of Pol5 protein in the regulation of Schizosaccharomyces pombe (S. pombe) viability. Immunoprecipitation and mass spectrometry assays identified Eso1 protein binding to Cdc2, Pol5 and Cdc21, as well as other proteins. Pol5 protein specifically bound to Eso1 protein, but not to the Rad30 part or Rad30 part plus the additional zinc finger domain of Eco1 protein. Mass spectrometry data further identified several acetylation or trimethylation modification sites in the lysine residues of the Pol5 protein. However, the mutation of the Pol5 K47 site to arginine was lethal to S. pombe. Eso1 protein was able to acetylate Pol5 protein and mediate S. pombe viability. On the whole, our data indicate that the Eso1 interaction with Pol5 which acetylates Pol5 protein is essential for S. pombe viability.


Assuntos
Proteínas de Ciclo Celular/genética , Sobrevivência Celular/genética , DNA Polimerase Dirigida por DNA/genética , Proteínas de Schizosaccharomyces pombe/genética , Schizosaccharomyces/genética , Acetilação , Sequência de Aminoácidos/genética , Proteína Quinase CDC2/genética , Cromátides/genética , Componente 4 do Complexo de Manutenção de Minicromossomo/genética , Ligação Proteica
6.
Forensic Sci Int ; 259: 247.e1-6, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26778588

RESUMO

In forensic anthropology, facial soft tissue depth measurement is crucial for craniofacial reconstruction technology, which is based on the morphological features of human faces to rebuild appearances of decedents, helps forensic scientists to identify the nameless bone. We measured the facial tissue depth of 135 young subjects from northern China whereby revealing the relationship among tissue depth, sex and BMI as well as providing data for craniofacial reconstruction in forensic science. All the volunteers are healthy medical students including 64 males and 71 females. Ultrasound was used to measure 19 points across the face evenly distributed in 6 regions including the eye, nose, mouth, cheek, jaw and chin. Our results indicate that tissue thickness at 11 points of females and 11 points of males are related to BMI. A majority of points are thicker in females than those of males. Further comparisons with data of American and European population show an apparent diversity in both genders.


Assuntos
Pesos e Medidas Corporais/métodos , Face/anatomia & histologia , Face/diagnóstico por imagem , Adulto , Índice de Massa Corporal , China , Etnicidade , Feminino , Humanos , Masculino , Fatores Sexuais , Adulto Jovem
7.
Cell Biol Int ; 38(5): 682-8, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24375893

RESUMO

In Schizosaccharomyces pombe, Eso1p is a protein fusion. Two-thirds of its N-terminus is conserved to budding yeast Rad30, which functions in error-free replication of UV-damaged DNA. A third of the C-terminus is highly conserved to budding yeast Eco1, a lysine acetyltransferase, which is essential for the establishment of cohesion. Both Rad30p and Eco1p need to be finely tuned in budding yeast. Given the distinct function existed in Rad30p and Eco1p, it is enigmatic how the Eso1p, the protein fusion regulated in S. pombe, works. We have identified two forms of the Eso1 protein by Western blot, and detected the Eco1-homology fragment by M/S analysis following TAP purification of Eso1 protein. The result raises the possibility that Eso1 might be processed in vivo to release the Eco1-homology fragment, which allows the independent regulation of Rad30-homology and Eco1-homology fragments.


Assuntos
Proteínas de Ciclo Celular/genética , Proteínas de Schizosaccharomyces pombe/genética , Schizosaccharomyces/genética , Sequência de Aminoácidos , Dados de Sequência Molecular , Isoformas de Proteínas/genética
8.
J Forensic Leg Med ; 20(4): 321-5, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23622483

RESUMO

Bloodstain age is a parameter that can be used in crime scene investigations. Bloodstain age can be determined by measuring the 18S rRNA:ß-actin mRNA ratio by Reverse Transcription-quantitative PCR (RT-qPCR). Since this ratio is a function of time, it can be used as an estimator of bloodstain age. However, it is important to validate the technique in a variety of scenarios before it can be applied. We investigated 18S rRNA:ß-actin mRNA ratio in bloodstains from sixteen Chinese subjects in 28 days under uncontrolled room conditions. The ratio changed in a linear fashion. It was also found that the subjects' gender affected the relationship between time and the RNA ratio.


Assuntos
Actinas/genética , Manchas de Sangue , RNA Mensageiro/sangue , RNA Ribossômico 18S/genética , Adulto , Feminino , Medicina Legal/métodos , Humanos , Modelos Lineares , Masculino , Reação em Cadeia da Polimerase em Tempo Real , Fatores Sexuais , Manejo de Espécimes , Fatores de Tempo , Adulto Jovem
9.
Acta Pharmacol Sin ; 25(1): 68-75, 2004 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-14704125

RESUMO

AIM: To observe the effects of three cytokines on the apoptosis of Tf-1 cells induced by gamma irradiation and investigate the relationship between apoptosis and caspase-3 activity. METHODS: Different cytokines GM-CSF, IL-3 and GM-CS/IL-3 fusion protein were added into the irradiated Tf-1 cells. MTT assay, morphology, flow cytometry, and DNA fragmentation assay were used to observe the effects of cytokines on apoptosis. The caspase-3 activity was determined with a fluorocytometer. RESULTS: Irradiated Tf-1 cells showed typical morphological characteristic of apoptosis demonstrated by transmission electron microscopy and were accumulated in G0/G1 phase. In the groups treated with growth factors after irradiation, three cytokines significantly increased the viability rate, distinctly decreased the apoptosis rate and the proportion of DNA fragmentation. When Tf-1 cells were irradiated by gamma irradiation, caspase-3 activity was increased at different time points. In comparison with the control group in which no growth factor was added after the cells were irradiated, the caspase-3 activity of irradiated Tf-1 cells was significantly inhibited by addition of the above cytokines. Thirty-six hours after irradiation, in the control group, GM-CSF, IL-3, GM-CSF and IL-3 in combination, and two GM-CSF/IL-3 fusion protein groups, the apoptosis rate was 73 %, 11 %, 15 %, 13 %, 12 %, and 13 %. The percent of fragmented DNA was 36 %, 19 %, 18 %, 14 %, 13 %, and 14 %. The fluorescence intensity was 16923, 5529, 6581, 5322, 5426, and 5485. CONCLUSION: GM-CSF, IL-3, and GM-CSF/IL-3 fusion protein could protect Tf-1 cells from apoptosis induced by gamma irradiation. After Tf-1 cells were irradiated, the caspase-3 activity was significantly increased but was dramatically decreased by the above cytokines. The remarkable inhibition of caspase-3 activity may be one of the mechanisms of these hematopoietic growth factors exerting their anti-apoptotic effects.


Assuntos
Apoptose/efeitos dos fármacos , Caspases/metabolismo , Fator Estimulador de Colônias de Granulócitos e Macrófagos/farmacologia , Interleucina-3/farmacologia , Apoptose/efeitos da radiação , Caspase 3 , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Radioisótopos de Césio , Fragmentação do DNA , Humanos , Leucemia Mieloide/patologia , Proteínas Recombinantes de Fusão/farmacologia
10.
Zhonghua Wai Ke Za Zhi ; 41(2): 96-8, 2003 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-12783667

RESUMO

OBJECTIVE: To evaluate the in vitro and in vivo function of anti-human bladder tumor human-mouse chimeric antibody ch-BDI and its future clinical application. METHODS: With ch-BDI in high-expression cell-line medium, affinity chromatography was used for the purification. Labeled with (99m)Tc through reduction method, its immunoreactive fraction and association constant were measured. The constant was injected into nude mice with xenografted human bladder tumor. The biodistribution of the labeled ch-BDI was studied with radioimmunoimaging. RESULTS: ch-BDI showed desirable immunoreactive fraction (76%) and association constant (3.56 x 10(9) M(-1)) in vitro and a terrific specific targeting effect in vivo. CONCLUSION: ch-BDI has fairly good function against human bladder tumor both in vitro and in vivo, and is promising in clinical use.


Assuntos
Anticorpos Monoclonais/imunologia , Anticorpos Antineoplásicos/imunologia , Proteínas Recombinantes de Fusão/imunologia , Neoplasias da Bexiga Urinária/imunologia , Animais , Afinidade de Anticorpos , Humanos , Técnicas In Vitro , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus
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