Effects of glucose and starch on the toxicity of nitrobenzene to plants and microbes in constructed wetlands.

Photosynthetic pigment content, antioxidant enzyme activities of plants, microbial enzyme activities and community structure were analyzed to investigate the effects of glucose and starch on the toxicity of nitrobenzene (NB) to plants and microbes in constructed wetlands (CWs). As the influent NB concentration increased from 10 mg/L to 100 mg/L, the NB removal efficiency of the blank group decreased from 97.1% to 75.02%. However, the NB removal efficiencies of the external carbon source groups were maintained at nearly 100%. External carbon sources accelerated the transformation process of NB to aniline (AN), thus decreasing NB toxicity to the microbes and plants. When the influent NB concentration reached 100 mg/L, the NB removal rates and NB reductase activities of the external carbon source groups were 2.4 times and 4 times higher, respectively, than those of the blank group. Most of the dominant genera found in the three CWs could reduce nitroaromatics to the corresponding aromatic amines according to the results of high-throughput sequencing. The performance of NB removal in the CWs indicated the potential of CWs for NB treatment and the necessity of external carbon sources under high NB concentrations.