Candidate Circulating Biomarkers of Spontaneous Miscarriage After IVF-ET Identified via Coupling Machine Learning and Serum Lipidomics Profiling.

Spontaneous miscarriage is a common pregnancy complication. Multiple etiologies have been proposed such as genetic aberrations, endocrinology disorder, and immunologic derangement; however, the relevance of circulating lipidomes to the specific condition remains unclear. In the present study, lipidomics profiling was examined on serum of women with spontaneous miscarriage after in vitro fertilization and embryo transfer (IVF-ET). Screening and analysis of differential lipid levels were conducted using a machine learning approach to verify the stability and validity of potential serum biomarkers. Seven lipid species presented significant differences between the abortion and term birth patients, including three types of sphingomyelins (SMs), two types of diglycerides (DGs), one phosphatidylcholine (PC), and one lysophosphatidylethanolamine (LPE). All the SMs presented with a fold change of > 1, while both the PC and LPE had a fold change of < 1. The DG containing two saturated fatty acyl chains was decreased, but that containing two unsaturated fatty acyl chains was increased in the miscarriage group compared to the control group. This study reveals the relevance of lipid profiles to spontaneous abortion after IVF-ET, providing potential biomarkers and therapeutic targets for the specific clinical scenario.

[Pregnancy outcomes of in vitro fertilization and embryo transfer in infertile women with polycystic ovarian syndrome].

OBJECTIVE: To investigate the pregnancy outcomes of in vitro fertilization (IVF) and embryo transfer (ET) in infertile women with polycystic ovarian syndrome (PCOS). METHODS: From August 2005 to June 2011, 200 IVF-ET cycles performed in women with polycystic ovarian syndrome in Shengjing Hospital and Shenyang 204 Hospital were enrolled in this retrospective study, matched with 400 IVF-ET cycles in infertile women with fallopian tube factors as control group. The incidence of abortion, gestational diabetes mellitus (GDM), hypertensive disorders in pregnancy, preterm birth, small for gestational age infant (SGA), large for gestational age infant (LGA), neonatal asphyxia, neonatal death and deformity was compared between two groups. RESULTS: The incidence of spontaneous abortion was 26.0% (52/200) in PCOS group, which was significantly higher than 10.2% (41/400) in control group (P < 0.05). The incidence of GDM, hypertensive disorders in pregnancy, preterm birth, cesarean section in PCOS group was 23.6% (35/148), 16.2% (24/148), 17.6% (26/148), 83.1% (123/148), which were significantly higher than 4.2% (15/359), 6.1% (22/359), 7.8% (28/359), 73.8% (265/359) in control group (P < 0.05). The incidence of SGA, LGA, neonatal asphyxia, neonatal death and deformity did not show remarkable difference between two groups, which were 2.7% (4/148), 4.7% (7/148), 5.4% (8/148), 0 in PCOS group and 1.4% (5/359), 2.2% (8/359), 2.8% (10/359), 0 in control group (P > 0.05). CONCLUSION: IVF-ET is an effective treatment for infertile women with PCOS, however, the incidence of spontaneous abortion, GDM, hypertensive disorders in pregnancy, preterm birth, cesarean section in PCOS patients was increased.