RESUMO
Serotonin-containing cytostructures of mouse bone marrow after autotransplantation were studied using Falk-Hillarp luminescent histochemical method, and expression of antiapoptotic marker was determined by the immunohistochemical reaction. Autotransplantation of the bone marrow in mice led to an increase in the number of mast cells; in 40 and 120 min after autotransplantation, serotonin content in mast cells increased by 12 and 23%, respectively, and in hemopoietic cells of the bone marrow - by 24 and 78%, respectively, which affected the early stages of proliferation and differentiation of hemopoietic cells. The mitotic index in bone marrow smears at these terms increased by 3.8 and 4.5 times. According to myelogram data, the fraction of early undifferentiated forms (lymphoblasts, erythroblasts) in the primary organ of hemopoiesis (bone marrow) increased.
Assuntos
Transplante de Medula Óssea/métodos , Hematopoese/fisiologia , Transplante Autólogo/métodos , Animais , Células da Medula Óssea/citologia , Células da Medula Óssea/metabolismo , Diferenciação Celular/genética , Diferenciação Celular/fisiologia , Hematopoese/genética , Masculino , Mastócitos/citologia , Mastócitos/metabolismo , CamundongosRESUMO
The effectiveness of acupuncture contributing to stimulation of morphological organization and altering the dynamics of histamine in the spleen of mice already in the first days after treatment was validated using the methods of luminescent and light microscopy.
Assuntos
Terapia por Acupuntura/métodos , Histamina/metabolismo , Baço/anatomia & histologia , Animais , Medições Luminescentes , Camundongos , Microscopia de Fluorescência , Espectrometria de Fluorescência , Baço/crescimento & desenvolvimento , Baço/metabolismoRESUMO
We studied the effects of ethanol on bioamine-containing structures in the jejunum at different stages of alcohol intoxication. The content of catecholamines, serotonin, and histamine in enterocytes of the villus epithelium, submucosa mast cells, crypt enterocytes, and muscular layer was measured by luminescent microscopy and cytospectrofluorometry. Uneven increase of biogenic amine content was found in rats in the initial period of chronic alcohol intoxication (60 days). Further alcohol intake (up to 180 days) impaired the balance of biogenic amines; catecholamines started to prevail.
Assuntos
Alcoolismo/metabolismo , Etanol/farmacologia , Mucosa Intestinal/metabolismo , Jejuno/inervação , Neurotransmissores/metabolismo , Animais , Catecolaminas/análise , Enterócitos , Histamina/análise , Mucosa Intestinal/efeitos dos fármacos , Jejuno/metabolismo , Jejuno/ultraestrutura , Mastócitos , Ratos , Serotonina/análiseRESUMO
Study of the effects of 2- and 10-min acupuncture on skin structures in acupuncture points on the upper limb and trunk of outbred albino rats showed that changes in the monoamine status of the skin appeared as early as 15 min after acupuncture. However, the effects in points GV14 and LI11 were different: skin structures in acupuncture point LI11 reacted sooner and more intensively. Acupuncture caused opposite changes in the serotonin index in different structures of the skin in acupuncture points.
